Adgrg2 (NM_001270872) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Gpr64; He6; Re6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217387 representing NM_001270872 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTTCTCTGGGGGTCAGTACAGCCCTGTTGGCAGGCCTGAAGAGGTTTTACTGATATACAAGATAT TCCTTGTCATCATTTGTTTTCATGCCATTCTTGTTACATCCCTGAAAGATGCTGCTTCCGAAGTATTGTC GACTTTAAACAGAACAGAAAAATCTAAAATCACTATATTAAAAACCTTCAATGCATCAGGAGTCAAATCC CAGAGAAATATCTGCAATTTGTCATCTATTTGCAGTGACTCAGTGTTTTTTAGAGGTGAGATAGTGTTTC AACATGATGACCACTACAACGTTACTCAGAATCAAGATATAGTTAACAGCACCTTCGCTGGAGTCCTGTC TCTAAGCGAACTGAAGCGAACAGAACTCAACAAAACTCTACAGACCTTAAGTGAGACTTACTTTATAGTG TGTGCTACCGCAGAGGCCCAAAACACATTAAACTGTACATTCACAGTAAAACTGAATGAGACCATGAATG TGTGTGCCATGATGGTTACTTTCAAAAGTGTACAGATTCGGCCAATGGAACAGTGCTGCTGTTCCCCCAG GACTCCCTGCCCTTCCTCGCCGGAAGAGTTAGAAAAACTACAGTGTGATCTGCAGGATCCCATTGTCTGC CTTGCCGATCAACCACATGGCCCACCAGTATCTTCTTCCAGCAAGCCTGTGCCAGTTGTACCTCAGGCCA CCATTTTTTCCCACGTTGCTAGTGACTTCTCTTTGGCTGAACCCCTTGATCATGCCCTTATGACCTCAAG CACACCCTCTCTGGCACAAGAGACACGCCTTCCATCTCCTCAGCCTACAATCTCCCTGACTTCCAGTCCT GCCATTGACTTGCCTGTTCAACATGTAGTGGCTTCTTCCTCCTTGCCCCAAACTGATCTTTCCCACACCC TATCACCTGTGCAGTCCTCCATTCCCTCTCCTACCACAGCAGCTCCATCTGTCCCTGAAAAAGTGGTCGC CATCAGCACACCTCCTGGCGAGACAGTTGTCAACACGAGTAGTGTTCCTGATCTGGAAGCCCAAGTATCT CAAATGGAGAAAGCTTTGTCTTTGGGCAGCTTGGAACCTAATCTTGCGGGTGAAATGGTGAACCGAGTCA GCAAACTCCTTCACTCTCCACTTGCCTTGCTAGCCCCTCTAGCTCAAAGGTTGCTAAAAGTGGTAGATGC CATTGGCTTACAGCTGAATTTTTCATCTACAACTATCAGTCTAACTTCTCCTTCTTTGGCTCTTGCTGTG ATCAGAGTGAATGCCAGTAATTTCAATACAACAACCTTTGCAGCCCAAGACCCAGCAAATCTCCAGGTGT CTCTGGAAGCCCAGGCTCCTAAGAATAGTATTGGCGCCATTACTCTACCCTCATCGCTGATGAGTAATTT GCCAGCTAGTGAGGTAGAATTGGCTTCGAGGGTTCAGTTCAATTTCTTTGAAACACCTGCCCTATTTCAG GACCCCTCCCTGGAGAACCTCTCTCTGATAAGCTATGTCATATCATCAAGTGTCACAAACATGACCATCA AGAACTTGACAAGAAACGTGACAGTTGCACTGAAGCACATCAACCCAAGTCAGGATGACTTAACTGTCAA ATGTGTTTTCTGGGACTTGAACAGAAATGGTGGCAGAGGAGGTTGGTCATCTGATGGCTGTTCCGTCAAA GAGAAGAGGATGAACGAAACTATCTGTACCTGTAGCCACCTTACAAGTTTTGGCATCCTATTGGATCTAT CTCGGACATCCTTACCACCAAGTCAAATGATGGCTCTGACATTTATCACGTATATTGGCTGTGGGCTTTC ATCAATTTTTCTGTCAGTTACTCTTGTAACCTATATAGCCTTTGAAAAGATCCGGAGGGATTACCCTTCC AAAATCCTCATTCAACTGTGTGCTGCCCTGCTTCTGCTCAACCTGGTCTTCCTCTTAGACTCCTGGATTG CACTGTATAATGCCCGAGGTTTCTGCATCTCCGTGGCTGTATTTCTTCACTATTTTCTCTTGGTATCATT CACATGGATGGGCTTAGAAGCATTTCATATGTACCTAGCACTGGTTAAGGTTTTTAATACTTACATCCGA AAGTACATCCTTAAATTCTGCATTGTTGGCTGGGGGATACCAGCTGTGGTTGTATCCATCGTCCTGACTA TATCCCCAGATAATTATGGGATTGGATCTTATGGGAAATTCCCCAATGGCACACCAGATGACTTTTGCTG GATCAACAGCAGTGTGGTGTTCTATATCACGGTTGTGGGATATTTCTGTGTGATATTTCTACTGAACGTC AGCATGTTCATTGTGGTCTTGGTTCAGCTCTGTCGAATTAAAAAGAAGAAGCAACTGGGAGCGCAGCGCA AAACTAGTATTCAAGACCTCCGGAGTATTGCTGGCCTCACATTTTTACTGGGAATTACTTGGGGCTTTGC CTTCTTTGCCTGGGGACCAGTTAATCTCACCTTCATGTATCTCTTTGCCATCTTTAACACCTTACAAGGC TTTTTCATATTCATCTTTTACTGTGCAGCAAAAGAGAATGTCAGAAAACAGTGGAGGCGGTATCTTTGTT GTGGAAAATTACGACTGGCTGAAAACTCTGACTGGAGTAAAACTGCTACTAATGGTTTAAAGAAGCAGAC TGTAAATCAAGGAGTATCCAGCTCTTCAAATTCCTTACAGTCAAGCTGTAACTCCACTAACTCCACTACA CTCCTAGTGAATAGTGATTGCTCAGTGCACGCAAGCGGGAATGGCAATGCATCTACCGAGAGGAATGGGG TTTCTTTCAGTGTTCAGAATGGAGACGTGTGCCTTCATGATCTCACTGGGAAACAGCACATGTTTAGTGA CAAAGAGGACTCCTGCAATGGCAAAAGCCGGATGGCACTCAGAAGGACTTCAAAGCGGGGAAGCTTACAC TTTATCGAGCAAATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270872
Insert Size	2958 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270872.1, NP_001257801.1
RefSeq Size	4524 bp
RefSeq ORF	2958 bp
Locus ID	266735
UniProt ID	Q8CJ11
Gene Summary	epididymus specific member of the LNB-TM7 subfamily of seven transmembrane receptors [RGD, Feb 2006] Transcript Variant: This variant (3) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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