Sh3gl2 (NM_053935) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sh3d2a; Sh3p4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216451 representing NM_053935 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGGCAGGGCTGAAGAAGCAGTTCCACAAAGCCACTCAGAAAGTGAGTGAGAAGGTGGGAGGAG CCGAAGGCACCAAGCTCGATGATGACTTCAAAGAGATGGAGAGGAAAGTGGATGTTACCAGCAGGGCCGT GATGGAGATAATGACGAAAACAATTGAATACCTCCAACCCAATCCAGCTTCCAGGGCTAAACTCAGTATG ATCAACACCATGTCGAAAATCCGTGGCCAAGAGAAGGGGCCAGGCTATCCTCAGGCAGAAGCACTGCTGG CAGAGGCTATGCTCAAGTTCGGCAGGGAGCTCGGCGATGATTGCAACTTTGGTCCGGCTCTTGGTGAGGT AGGAGAAGCCATGAGGGAGCTCTCGGAGGTCAAGGACTCTTTGGACATGGAAGTGAAGCAGAACTTCATC GACCCCCTTCAGAATCTTCATGACAAAGATCTGAGGGAGATTCAGCATCACCTGAAAAAGCTGGAAGGCC GACGCTTAGACTTTGATTATAAGAAGAAGCGACAAGGAAAGATTCCAGACGAAGAACTCCGCCAAGCTCT GGAGAAATTTGATGAGTCTAAAGAAATCGCTGAGTCGAGCATGTTCAACCTCTTGGAGATGGATATTGAA CAGGTGAGCCAGCTCTCTGCACTTGTTCAGGCTCAGCTGGAGTACCACAAACAGGCTGTGCAGATCCTGC AACAGGTCACTGTCAGGCTGGAAGAGAGAATAAGACAAGCTTCATCTCAACCAAGAAGGGAATATCAGCC CAAACCACGAATGAGCCTAGAGTTTGCCACTGGAGACGGTACGCAGCCCAACGGGGGTCTCTCCCACACA GGCACACCCAAACCTGCAGGTGTCCAAATGGATCAGCCTTGCTGCCGAGCCCTGTATGACTTTGAACCTG AAAATGAAGGGGAGTTGGGTTTTAAAGAGGGTGATATCATCACGCTCACTAATCAGATTGATGAGAACTG GTATGAGGGGATGCTTCATGGCCAGTCCGGCTTTTTCCCCATCAACTATGTAGAAATTCTGGTTGCTCTG CCCCATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053935
Insert Size	1059 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053935.1, NP_446387.1
RefSeq Size	1491 bp
RefSeq ORF	1059 bp
Locus ID	116743
UniProt ID	O35179
Gene Summary	interacts with synaptojanin and dynamin I; may be involved in synaptic vesicle recycling [RGD, Feb 2006]

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