Nrg1 (NM_031588) Rat Untagged Clone
CAT#: RN215433
Nrg1 (untagged ORF) - Rat neuregulin 1 (Nrg1), (10 ug)
CNY 8,270.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN215433 representing NM_031588
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAGCGCAAAGAAGGCAGAGGCAAGGGGAAGGGCAAGAAGAAGGACCGGGGATCCCGCGGGAAGC CCGGGCCCGCCGAGGGCGACCCGAGCCCAGCACTGCCTCCCAGATTGAAAGAAATGAAGAGCCAGGAGTC AGCTGCAGGCTCCAAGCTAGTGCTCCGGTGCGAAACCAGCTCCGAGTACTCCTCACTCAGATTCAAATGG TTCAAGAATGGGAACGAGCTGAACCGCAAAAATAAACCAGAAAACATCAAGATACAGAAGAAGCCAGGGA AGTCAGAGCTTCGAATTAACAAAGCATCCCTGGCTGACTCTGGAGAGTATATGTGCAAAGTGATCAGCAA GTTAGGAAATGACAGTGCCTCTGCCAACATCACCATTGTTGAGTCAAACGAGTTCATCACTGGCATGCCA GCCTCGACTGAGACAGCCTATGTGTCCTCAGAGTCTCCCATTAGAATCTCAGTTTCAACAGAAGGCGCAA ACACTTCTTCATCCACATCGACATCCACGACTGGGACCAGCCATCTCATAAAGTGCGCGGAGAAGGAGAA AACTTTCTGTGTGAATGGGGGCGAGTGCTTCACGGTGAAGGACCTGTCAAACCCGTCAAGATACTTGTGC AAGTGCCCAAATGAGTTTACTGGTGATCGTTGCCAAAACTACGTAATGGCCAGCTTCTACAAAGCGGAGG AACTCTACCAGAAGAGGGTGCTGACAATTACTGGCATCTGTATCGCCCTGCTGGTGGTCGGCATCATGTG TGTGGTGGCCTACTGCAAAACCAAGAAGCAGCGGCAGAAGCTTCATGATCGGCTTCGGCAGAGTCTTCGG TCAGAACGGAGCAACCTGGTGAACATAGCGAATGGGCCTCACCACCCAAACCCACCGCCAGAGAACGTGC AGCTGGTGAATCAATACGTATCTAAAAACGTCATCTCCAGTGAGCATATTGTTGAGAGAGAAGTGGAGAC TTCCTTTTCCACCAGTCATTACACTTCCACAGCCCATCACTCCACGACTGTCACCCAGACTCCTAGTCAC AGCTGGAGTAATGGGCACACGGAGAGCATCATTTCAGAAAGCAACTCCGTAATCATGATGTCTTCGGTAG AGAACAGCAGGCACAGCAGTCCCGCCGGGGGCCCACGAGGACGTCTTCATGGCCTGGGAGGCCCTCGTGA TAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGACTCCTACAGAGACTCTCCTCATAGCGAAAGGTAT GTATCAGCCATGACCACCCCGGCTCGTATGTCACCTGTAGATTTCCACACGCCAAGCTCCCCTAAATCGC CCCCTTCGGAAATGTCTCCACCCGTGTCCAGCATGACGGTGTCAATGCCCTCTGTGGCAGTCAGCCCCTT TGTGGAAGAAGAGAGGCCTCTGCTGCTTGTGACGCCACCAAGGCTACGGGAGAAGAAATATGATCATCAC CCCCAGCAACTCAACTCCTTTCATCACAACCCTGCACATCAGAGTACCAGCCTCCCCCCTAGCCCACTGA GGATAGTGGAGGATGAGGAGTACGAGACGACCCAGGAGTATGAGTCAGTTCAAGAGCCCGTTAAGAAAGT CACCAATAGCCGGCGGGCCAAAAGAACCAAGCCCAATGGCCACATTGCCAATAGGTTGGAAATGGACAGC AACACAAGTTCTGTGAGCAGTAACTCAGAAAGTGAGACAGAAGACGAAAGAGTAGGTGAAGACACACCAT TCCTGGGCATACAGAACCCCCTGGCAGCCAGCCTTGAGGTGGCCCCTGCCTTCCGTCTGGCTGAGAGCAG GACTAACCCAGCAGGCCGCTTCTCCACACAGGAGGAATTACAGGCCAGGCTGTCTAGTGTAATCGCTAAC CAAGACCCTATTGCTGTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_031588 |
Insert Size | 1911 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_031588.2, NP_113776.2 |
RefSeq Size | 3246 bp |
RefSeq ORF | 1911 bp |
Locus ID | 112400 |
Gene Summary | ligand for Erbb3 and Erbb4 receptors; gene produces many different alternative splicing isoforms; involved in neural and organ development [RGD, Feb 2006] Transcript Variant: This variant (13) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (13) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR215433 | Nrg1 (Myc-DDK-tagged ORF) - Rat neuregulin 1 (Nrg1), (10 ug) |
CNY 7,104.00 |
|
RR215433L3 | Lenti ORF clone of Nrg1 (Myc-DDK-tagged ORF) - Rat neuregulin 1 (Nrg1), (10 ug) |
CNY 9,980.00 |
|
RR215433L4 | Lenti ORF clone of Nrg1 (mGFP-tagged ORF) - Rat neuregulin 1 (Nrg1), (10 ug) |
CNY 10,930.00 |