Nrg1 (NM_031588) Rat Untagged Clone

CAT#: RN215433

Nrg1 (untagged ORF) - Rat neuregulin 1 (Nrg1), (10 ug)



  "NM_031588" in other vectors (3)

CNY 8,270.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN215433 representing NM_031588
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGAGCGCAAAGAAGGCAGAGGCAAGGGGAAGGGCAAGAAGAAGGACCGGGGATCCCGCGGGAAGC
CCGGGCCCGCCGAGGGCGACCCGAGCCCAGCACTGCCTCCCAGATTGAAAGAAATGAAGAGCCAGGAGTC
AGCTGCAGGCTCCAAGCTAGTGCTCCGGTGCGAAACCAGCTCCGAGTACTCCTCACTCAGATTCAAATGG
TTCAAGAATGGGAACGAGCTGAACCGCAAAAATAAACCAGAAAACATCAAGATACAGAAGAAGCCAGGGA
AGTCAGAGCTTCGAATTAACAAAGCATCCCTGGCTGACTCTGGAGAGTATATGTGCAAAGTGATCAGCAA
GTTAGGAAATGACAGTGCCTCTGCCAACATCACCATTGTTGAGTCAAACGAGTTCATCACTGGCATGCCA
GCCTCGACTGAGACAGCCTATGTGTCCTCAGAGTCTCCCATTAGAATCTCAGTTTCAACAGAAGGCGCAA
ACACTTCTTCATCCACATCGACATCCACGACTGGGACCAGCCATCTCATAAAGTGCGCGGAGAAGGAGAA
AACTTTCTGTGTGAATGGGGGCGAGTGCTTCACGGTGAAGGACCTGTCAAACCCGTCAAGATACTTGTGC
AAGTGCCCAAATGAGTTTACTGGTGATCGTTGCCAAAACTACGTAATGGCCAGCTTCTACAAAGCGGAGG
AACTCTACCAGAAGAGGGTGCTGACAATTACTGGCATCTGTATCGCCCTGCTGGTGGTCGGCATCATGTG
TGTGGTGGCCTACTGCAAAACCAAGAAGCAGCGGCAGAAGCTTCATGATCGGCTTCGGCAGAGTCTTCGG
TCAGAACGGAGCAACCTGGTGAACATAGCGAATGGGCCTCACCACCCAAACCCACCGCCAGAGAACGTGC
AGCTGGTGAATCAATACGTATCTAAAAACGTCATCTCCAGTGAGCATATTGTTGAGAGAGAAGTGGAGAC
TTCCTTTTCCACCAGTCATTACACTTCCACAGCCCATCACTCCACGACTGTCACCCAGACTCCTAGTCAC
AGCTGGAGTAATGGGCACACGGAGAGCATCATTTCAGAAAGCAACTCCGTAATCATGATGTCTTCGGTAG
AGAACAGCAGGCACAGCAGTCCCGCCGGGGGCCCACGAGGACGTCTTCATGGCCTGGGAGGCCCTCGTGA
TAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGACTCCTACAGAGACTCTCCTCATAGCGAAAGGTAT
GTATCAGCCATGACCACCCCGGCTCGTATGTCACCTGTAGATTTCCACACGCCAAGCTCCCCTAAATCGC
CCCCTTCGGAAATGTCTCCACCCGTGTCCAGCATGACGGTGTCAATGCCCTCTGTGGCAGTCAGCCCCTT
TGTGGAAGAAGAGAGGCCTCTGCTGCTTGTGACGCCACCAAGGCTACGGGAGAAGAAATATGATCATCAC
CCCCAGCAACTCAACTCCTTTCATCACAACCCTGCACATCAGAGTACCAGCCTCCCCCCTAGCCCACTGA
GGATAGTGGAGGATGAGGAGTACGAGACGACCCAGGAGTATGAGTCAGTTCAAGAGCCCGTTAAGAAAGT
CACCAATAGCCGGCGGGCCAAAAGAACCAAGCCCAATGGCCACATTGCCAATAGGTTGGAAATGGACAGC
AACACAAGTTCTGTGAGCAGTAACTCAGAAAGTGAGACAGAAGACGAAAGAGTAGGTGAAGACACACCAT
TCCTGGGCATACAGAACCCCCTGGCAGCCAGCCTTGAGGTGGCCCCTGCCTTCCGTCTGGCTGAGAGCAG
GACTAACCCAGCAGGCCGCTTCTCCACACAGGAGGAATTACAGGCCAGGCTGTCTAGTGTAATCGCTAAC
CAAGACCCTATTGCTGTATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_031588
Insert Size 1911 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_031588.2, NP_113776.2
RefSeq Size 3246 bp
RefSeq ORF 1911 bp
Locus ID 112400
Gene Summary ligand for Erbb3 and Erbb4 receptors; gene produces many different alternative splicing isoforms; involved in neural and organ development [RGD, Feb 2006]
Transcript Variant: This variant (13) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (13) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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