Prkacb (NM_001077645) Rat Untagged Clone

CAT#: RN215333

Prkacb (untagged ORF) - Rat protein kinase, cAMP dependent, catalytic, beta (Prkacb), (10 ug)



  "NM_001077645" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN215333 representing NM_001077645
Red=Cloning site Blue=ORF Orange=Stop codon

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGGGGAACACGGCGATCGCCAAGAAAGGCAGCGAAGTGGAGAGCGTGAAAGAATTCCTAGCCAAAGCCA
AAGAAGACTTTCTGAGGAAATGGGAGAACCCTCCCCCGAGTAATGCTGGGCTTGAGGATTTTGAAAGGAA
AAAAACCCTTGGAACGGGTTCCTTCGGAAGAGTCATGCTGGTAAAGCATAAAGCCACTGAGCAGTACTAC
GCCATGAAGATCTTAGACAAGCAGAAGGTCGTTAAGCTAAAGCAAATAGAGCACACTCTGAATGAGAAGA
GAATCCTGCAGGCAGTGGAGTTCCCGTTCCTTGTTCGGCTGGAGTATTCTTTTAAGGATAATTCTAATTT
ATACATGGTTATGGAATACGTTCCTGGGGGGGAAATGTTTTCACATCTAAGAAGAATTGGAAGGTTCAGT
GAACCCCATGCTCGTTTCTATGCAGCTCAGATCGTGCTAACATTTGAGTACCTCCATTCCCTCGACCTCA
TCTACAGAGATCTCAAGCCGGAAAACCTCTTAATTGACCACCAGGGTTACATCCAGGTCACAGATTTTGG
GTTCGCCAAGAGAGTCAAGGGCAGGACTTGGACATTGTGTGGCACCCCAGAGTACCTGGCCCCAGAGATC
ATCCTCAGCAAGGGTTACAATAAGGCAGTGGACTGGTGGGCATTGGGTGTACTGATCTACGAGATGGCTG
CTGGCTACCCCCCGTTCTTTGCTGACCAGCCAATTCAGATTTATGAGAAGATTGTTTCCGGAAAGGTCCG
CTTCCCGTCGCACTTCAGTTCCGATCTCAAGGACCTTCTGCGGAATCTGCTGCAGGTGGATCTGACAAAG
CGGTTTGGGAACCTGAAGAATGGCGTGAGTGACATCAAGACCCACAAGTGGTTTGCTACGACCGACTGGA
TCGCGATTTACCAGAGAAAGGTTGAGGCTCCATTCATACCAAAATTCAGAGGCTCTGGCGATACCAGCAA
CTTCGATGACTATGAAGAAGAAGAAATCCGAGTGTCTATAACAGAAAAATGTGGAAAGGAGTTTTGTGAA
TTTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites AscI-MluI     
ACCN NM_001077645
Insert Size 1056 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001077645.1, NP_001071113.1
RefSeq Size 1122 bp
RefSeq ORF 1056 bp
Locus ID 293508
UniProt ID P68182
Gene Summary Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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