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Prmt7 (NM_001014153) Rat Untagged Clone
CAT＃: RN214544
Prmt7 (untagged ORF) - Rat protein arginine methyltransferase 7 (Prmt7), (10 ug)
CNY 8,930.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1304869 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN214544 representing NM_001014153
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTCTTCTGTGGCCGTGCCAATCCAACCACGGGATCCCTGGAATGGCTGGAGGAGGATGAACACT ATGATTACCACCAAGAAATTGCCAGGTCATCCTATGCCGATATGCTACACGACAAAGACAGAAATATAAA ATATTACCAGGGTATCCGGGCAGCTGTGAGCAGAGTGAAAGACAAAGGACAGAAGGCCTTGGTTCTTGAC ATTGGCACTGGCACAGGCCTCTTATCAATGATGGCAGTTACCGCAGGGGCTGACTTCTGCTATGCTGTTG AGGTTTTTAAGCCAATGGCTGAGGCTGCTGTGAAGATTGTGGAGAAAAATGGCTTCAGTGATAAGATTAA AGTAATTAACAAGCATTCCACTGAGGTGACGGTTGGACCAGATGGTGACTTGCCATGCCGTGCTAACATT CTGGTTACGGAGCTGTTTGACACAGAGCTGATTGGGGAGGGAGCACTGCCCTCTTATGAGCACGCACACA AGCATCTTGTACAGGAAGACTGTGAAGCAGTGCCTCACAGGGCGACTGTCTATGCCCAGTTGGTGGAGTC CAAAAGGATGTGGTCATGGAACAAGCTGTTTCCTGTCCGGGTCCAGACTGGTCTTGGAGAGCAGCTTATC ATTCCTCCCTCAGAATTGGAGAGGTGTCCTGGTGCACCTTCCGTCTATGACATTCAGCTGAACCAGGTGT CACCTGCTGACTTCACTGTCCTCAGTGATGTGCTGCCAATGTTCAGCGTGGACTTCAGCAAGCAAGTCAG CAGCTCGGCAGCGTGCCATAGCAAGCAGTTTGTCCCTCTGGCATCTGGCCAAGCACAGGTGGTTCTCTCC TGGTGGGACATAGAAATGGACCCTGAGGGCAAGATCAAGTGCACCATGGCACCCTTTTGGGCACAGACAG ATCCACAGGAACTTCAGTGGCGGGACCACTGGATGCAGTGTGTGTACTTCCTGCCCCAGGAGGAGCCCAT TATGCAGGGCTCACCCCGATGCCTGGCTGCTCACCATGATGACTACTGTGTGTGGTACAGCCTTCAGAGA ACCAGCCCTGATGAGAATAACAGTGCCTACCAAGTTCGACCTGTATGTGACTGTCAGGCTCACCTGCTCT GGAACCGGCCACGGTTTGGAGAGATCAATGATCAGGACAGAACTGATCACTATGCCCGAGCCCTGAGGAC TATGCTGATGCCAGGTAGCATCTGCCTTTGTGTGAGTGACGGCAGTCTACTCTCTGTGTTGGCCCATCAC CTTGGAGCAGAGCAGGTGTTTACAGTCGAGAGTTCAGTAGCTTCCTATAGACTGATGAAAAGGATCTTCA AGGTTAACCACTTGGAAGATAAAATTACCGTCATCAATAAACGGCCTGAGTTGTTGACATCTGCAGACCT GGAGGGTAAGAAGGTCTCCCTCCTCCTGGGTGAACCTTTTTTCACCACCAGCCTGCTACCATGGCACAAC CTGTACTTCTGGTATGTGCGCACCTCTGTGGACCAGCACCTAGCGCCTGGGGCTGTGGTGATGCCCCAGG CAGCCTCACTGCATGCCGTGATCGTGGAGTTCAGGGACCTGTGGCGAATCCGGAGTCCTTGTGGTGACTG TGAAGGGTTTGACGTCCACATCATGGATGATATGATCAAGCACTCCCTGGATTTCCGAGAGAGCAGGGAG GCAGAGCCACAGCCACTGTGGGAATACCCCTGCAGAAGCCTCTCAGAGCCTCGACAGATCCTGACTTTTG ATTTCCAGCAGCCCATCCCCCAGCAGCCTATGCAATCCAGGGGCGTGATGGAACTGAGGAGGCCCGGGAA GAGCCATGGAGCTGTCCTGTGGATGGAATACCAGCTGACTCCAGATAGCACTGTCAGCACCGGCCTCATG AACCCTGCAGAGGACAAGGGGGACTGCTGCTGGAACCCCCACTGCAAGCAAGCTGTGTACTTCCTCAGTG CCACACTGGATCCTAGCGCGCCTCTGGATGGCCCTCAATCAGTCAGCTATGCTGTGGAGTTTCACCCCCT CACTGGAGACATCACCATGGAGTTTAGGCTTGCAGACGACACCTTGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001014153 |
| Insert Size | 2082 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014153.1, NP_001014175.1 |
| RefSeq Size | 2325 bp |
| RefSeq ORF | 2082 bp |
| Locus ID | 361402 |
| UniProt ID | Q5U4E8 |
| Gene Summary | Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR214544 | Prmt7 (Myc-DDK-tagged ORF) - Rat protein arginine methyltransferase 7 (Prmt7), (10 ug) |
CNY 8,930.00 |
|
| RR214544L3 | Lenti ORF clone of Prmt7 (Myc-DDK-tagged ORF) - Rat protein arginine methyltransferase 7 (Prmt7), (10 ug) |
CNY 10,740.00 |
|
| RR214544L4 | Lenti ORF clone of Prmt7 (mGFP-tagged ORF) - Rat protein arginine methyltransferase 7 (Prmt7), (10 ug) |
CNY 11,780.00 |
