Ank1 (NM_001107322) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206492 representing NM_001107322 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAACGGCCGCGCAGGTCGGGGTCAGACCCCGCGGCCGATGCTGCTACCAGCTTTTTGCGGGCGG CACGCTCAGGAAACCTGGACAAGGCTTTGGATCACCTACGCAATGGAGTGGACATTAACACCTGTAACCA GAACGGGTTGAATGGCTTGCATCTGGCCTCCAAAGAAGGCCATGTGAAGATGGTGGTTGAACTTCTGCAC AAAGAGATCATTCTAGAAACAACAACCAAGGTAAGGGATGGCTTCACTCCGCTGGCTGTGGCTCTGCAGC AGGGCCATGAGAATGTGGTGGCTCACCTCATCAACTATGGAACGAAAGGAAAAGTGCGCCTCCCTGCCCT GCACATCGCGGCCCGCAACGATGACACACGGACAGCCGCAGTCCTTCTGCAGAATGACCCCAACCCAGAT GTGCTTTCCAAGACGGGATTCACACCCCTCCACATCGCAGCACACTACGAGAACCTCAACGTGGCCCAGC TGCTCCTCAACCGGGGAGCCAGCGTCAACTTCACACCTCAGAATGGTATTACTCCACTGCACATCGCCTC CCGCAGGGGAAATGTGATCATGGTGAGACTCCTGCTGGACCGAGGGGCTCAGATAGAAACGAGGACCAAG GATGAACTGACACCACTCCACTGTGCAGCTCGGAATGGACACGTGAGAATCTCAGAGATCCTGCTGGACC ACGGGGCACCCATCCAAGCCAAAACCAAGAATGGCTTGTCCCCCATCCACATGGCGGCTCAGGGAGACCA CCTCGACTGTGTCCGACTTCTATTGCAATACAACGCCGAGATAGATGACATCACCTTAGATCACCTGACT CCTCTCCACGTGGCAGCCCACTGCGGCCACCACAGGGTGGCTAAGGTTCTTTTGGATAAAGGGGCCAAGC CGAACTCCAGAGCCCTGAATGGCTTTACCCCACTACACATCGCCTGCAAGAAGAACCACATACGTGTCAT GGAGCTGCTGCTGAAGACAGGAGCCTCTATTGACGCAGTCACTGAGAAAGTAGAAACCCCATTGCACATG GCAGCCCGAGCAGGGCATACAGAAGTGGCCAAATATTTGCTCCAGAACAAAGCCAAAGCCAATGCCAAGG CCAAGGACGACCAGACACCACTTCACTGTGCTGCTCGCATCGGCCACACGAGCATGGTGAAGCTCCTGCT GGAGAATGACGCCAGCCCCAACCTGGCTACCACTGCTGGCCACACACCTCTGCACACCGCAGCCCGTGAG GGTCACGTGGACACAGCCCTGGCCCTGCTGGAAAAGGAGGCATCCCAAGCCTGCATGACCAAGAAAGGAT TTACCCCTCTCCACGTGGCTGCCAAGTATGGGAAGGTCCGGCTGGCAGAGCTGCTGCTGGAACATGATGC CCATCCCAACGCGGCCGGAAAGAACGGCTTGACTCCCCTGCATGTGGCCGTCCATCACAACAACTTGGAC ATTGTCCAGCTTCTCCTTCCCCGAGGTGGCTCCCCGCACAGCCCTGCCTGGAATGGCTACACCCCTTTGC ACATCGCAGCCAAGCAGAACCAGATAGAGGTGGCCCGCAGTCTACTGCAATACGGAGGGTCCGCGAATGC AGAGTCTGTACAAGGAGTGACCCCGCTTCACCTGGCTGCCCAAGAGGGCCACACGGAAATGGTCGCTCTT CTCCTGTCAAAGCAAGCCAATGGCAACCTGGGGAACAAGAGTGGACTCACTCCTCTCCACCTGGTAGCAC AAGAAGGCCATGTTCCAGTGGCGGATGTGCTGATTAAACATGGCGTCACAGTGGATGCTACGACCCGGAT GGGTTACACCCCACTCCACGTGGCCAGTCACTACGGAAACATCAAGCTGGTGAAGTTCCTGCTACAGCAC CAGGCAGATGTCAACGCCAAGACCAAGCTAGGGTACAGCCCCTTGCACCAGGCAGCTCAGCAAGGACACA CGGACATTGTGACATTGCTCCTGAAGAATGGTGCTTCTCCAAATGAGGTCAGCTCGAACGGAACCACACC TCTGGCAATAGCCAAACGTTTGGGCTACATCTCTGTAACAGACGTGCTCAAGGTGGTCACAGATGAAACC AGTGTGGTGTTAGTCAGTGACAAACACCGGATGAGCTACCCTGAGACAGTGGATGAGATCCTGGATGTGT CTGAAGATGAAGGAACTGCTCACATATCTTTAACGGGTGACGAACTTGTCGGCTCGAAGGCTGAAAGGCG GGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTGGATTTTGTGCCGAAGCTAGACCAAGTGGTGGAA TCCCCAGCCATCCCAAGGATCCCTTGTGTCACGCCTGAGACTGTTGTGATCCGATCCGAAGATCAGGAAC AAGCATCTAAAGAGTATGATGAGGACTCACTCATTCCCAGTAGCCCAGCCACAGAGACCTCAGACAACAT CAGCCCCGTGGCTAGCCCCGTCCACACAGGGTTTCTGGTGAGCTTCATGGTCGATGCCCGGGGAGGATCC ATGAGAGGAAGTCGTCATAATGGCCTAAGGGTGGTGATCCCTCCCCGGACATGCGCAGCACCCACTCGCA TCACCTGCCGCCTGGTTAAGCCCCAGAAGCTAAGCACACCGCCCCCGCTGGCTGAGGAGGAGGGCCTAGC CAGCAGGATCATTGCACTGGGTCCTACGGGGGCCCAGTTCCTTAGCCCTGTGATCGTGGAAATCCCCCAC TTTGCCTCCCATGGCCGCGGGGACCGTGAGCTGGTGGTTCTGAGGAGCGAGAACGGCTCCGTGTGGAAGG AGCACAAGAGCCGCTATGGAGAAAGCTACCTGGACCAGATCCTGAACGGCATGGACGAAGAGCTGGGGAG CCTGGAGGAGCTGGAGAAGAAGCGCGTGTGTCGCATCATTACCACCGACTTCCCCCTGTACTTTGTCATC ATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTCCTGAAGGGGGCTCCCTGAGAAGCAAGCTGGTGC CCCTGGTACAGGCGACGTTCCCCGAAAACGCGGTGACCAAGAAAGTGAAGCTGGCTCTGCAGGCCCAGCC TGTCCCAGACGAGCTGGTGACCAAGCTCCTGGGCAACCAGGCCACCTTCAGCCCCATTGTTACTGTTGAG CCAAGGCGTCGGAAGTTCCACCGTCCCATTGGGCTGCGAATCCCACTCCCTCCCTCATGGACTGACAACC CCAGGGACAGCGGGGAGGGAGACACCACCAGCCTACGCTTACTGTGCAGCGTCATCGGTGGAACTGACCA AGCCCAGTGGGAAGATATAACGGGAACAACCAAGCTTGTATACGCCAATGAGTGCGCCAACTTTACCACC AACGTCTCAGCCAGATTCTGGCTGTCGGACTGTCCTCGGACCGCTGAGGCCGTACACTTTGCCACTCTCT TGTACAAAGAGCTCACGGCAGTTCCCTACATGGCCAAGTTTGTCATTTTCGCCAAGATGAACGACCCCCG GGAAGGACGGCTACGCTGCTACTGCATGACAGATGACAAGGTGGACAAGACCCTGGAACAGCATGAAAAC TTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTCTAGAAGGGATGCCTCTGTTTGCAGAACTCTCTG GGAACTTGGTGCCTGTCAAGAAAGCGGCCCAGCAGCGAAGCTTCCATTTCCAGTCATTTCGAGAGAATCG CCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGCGAGCCAGGAGGGTTCTTGTCGTTCCTGCGGAAG CCGATGAAATATGAAGACGCACAGCACATCCTCTGCCACTTGAATGTCACCATGCCCCCCTGTACCAAGG GCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACGCCCTTGACTCTTCGCTATAGCATTCTCAGCGAGTC CCGGCTGGGTTTTACCAGTGACACAGACCGTGTTGAAATGAAGATGGCTGTCATCAGGGAGCACCTCGGC CTCAGCTGGGCAGTGGAGAATCTGTACACGGCCCTGCGGAACATCGACAGAAGTGAGATTGTTAACATGT TGGAGGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGACCGGAGGCATGGGGACCGGGAGTACTCATT GTCACCCTCCCAGGTGAATGGTTACTCCTCGCTGCAGGACGAGCTGCTGTCCCCCGCCTCCCTGCACTAC GCACTTCCCTCTCCACTGTGTGCAGACCAGTACTGGAACGAAGTGGCCGTCATAGACGCCATCCCCCTGG CGGCCACGGAGCATGACACCATGCTGGAGATGTCTGACATGCAGGTGTGGTCTTCGGGCCTCACACCCTC CCTGGTCACTGCTGAGGACTCCTCTCTGGAGTGCAGCAAGGCCGAGGACTCTGACGCCATACCAGAGTGG AAGTTGGAGGGGGCACACTCAGAGGACACGCAGGGCCCGGAGCTGGGCTCTCAGGACCTGGTGGAGGACG ACACAGTGGATTCAGATGCCACAAATGGCCTGGCAGATTTGCTAGGCCAGCAGCGAGTTCACGCCCGAAT CACAGACTCACCCTCAGTGAGGCAGGTGCTGGACAGAAGCCAGGCCAGAACAGTGGACTGGTATAAACAG GGTTCAGCAGCATCATATCCACAAGAGGCCGCACAAAGTTCCTGGCAAGAGGAGGTCACGCAAGGCCCAC AGTCATTCCAGAGAAGGATCACCACCATCCAAGGGCCGGAGCCCGGCACACTTCAGGAATATGAGCAGGT GGTGGTGTCCACCAGGGAGCATGTGCAGAGGGGGCCACCTGAGAGCGACAGCCCCAAGGCCGGGAAGGAC CCAAGTCTGTGGGCATCGGAGAGTGCCTTCTCTCAAGAGGTGCAGGGGGATGAGCTTCAGAATATTCCAG GAGAGCAGGTGACAGAGGAACAATTCACAGACGAACAGGGCAACATTGTCACCAAGAAGATCATTCGAAA AGTCGTCCGACAGGTGGACTCCTCTGGTGCCATCGACACCCAGCAGCACGAGGAGGTGGAGCTAAGAGGG AGTGGACTCCAGCCGGACCTGATAGAAGGCAGGAAGGGGGCTCAAATAGTGAAGCGGGCCAGCTTGAAAA GGGGCAAACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107322
Insert Size	5124 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107322.2, NP_001100792.1
RefSeq Size	5124 bp
RefSeq ORF	5124 bp
Locus ID	306570

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