Tsc2 (NM_012680) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Rc
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203513 representing NM_012680 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAACCAACTAGCAAAGATTCAGGTTTGAAGGAGAAGTTCAAGATACTATTAGGACTGGGAACGT CAAGGCCAAATCCTAGGTGTGCAGAAGGCAAACAGACGGAGTTTATCATCACAGCGGAAATCTTGAGAGA ACTGAGTGGTGAATGCGGCCTCAACAATCGAATCCGGATGATAGGGCAGATCTGTGATGTGGCAAAAACT AAGAAACTTGAAGAGCATGCAGTGGAGGCACTTTGGAAGGCTGTCTCAGACTTGCTACAGCCAGAGCGGC CACCGGAGGCCCGACATGCAGTTCTGGCCTTGTTGAAGGCCATTGTGCAGGGACAGGGTGATCGTTTGGG GGTTCTCCGAGCCCTCTTCTTCAAAGTGATCAAGGACTATCCTTCCAACGAAGACCTCCATGAAAGGCTA GAGGTTTTTAAGGCCCTCACAGACAATGGAAGGCATATTACCTACTTGGAAGAAGAACTGGCAGAGTTTG TCCTGCAGTGGATGGATGTTGGCTTGTCCTCAGAATTCCTTCTGGTACTCGTGAACCTGGTCAAGTTCAA CAGCTGCTACCTTGACGAATACATTGCGCCAATGGTTCACATGATTTGTCTGTTATGTATCCGGACAGTG TCCTCTGTGGACATCGAGGTGTCCTTGCAAGTGCTGGATGCTGTGGTCTGCTACAACTGCCTGCCTGCTG AGAGCCTGCCTCTGTTCATTATCACCCTGTGCCGCACCGTCAACGTCAAGGAGCTGTGTGAGCCGTGCTG GAAGCTGATGCGAAACCTTCTAGGTACCCATCTAGGCCACAGTGCCATTTACAACATGTGCCGTATCATG GAGAACAGATCCTACATGGAAGACGCCCCCTTGTTGAGGGGAGCCGTGTTCTTTGTTGGGATGGCGCTCT GGGGAGCTCACCGACTCTATTCGCTCAAGAACTCCCCCACATCTGTGCTGCCGTCTTTTTATGAGGCCAT GACCTGTCCCAATGAGGTGGTGTCATATGAGATTGTTCTGTCCATAACAAGACTCATCAAGAAGTACAGG AAGGAGCTCCAGGCTGTGACATGGGACATTCTGCTGGACATTATTGAACGACTGCTCCAGCAACTCCAGA ACCTGGACAGCCCGGAACTCAGGACCATTGTCCATGACCTGCTGACCACTGTGGAGGAGCTGTGTGACCA GAACGAGTTCCACGGATCGCAAGAAAGATACTATGAACTGGTGGAGAGCTATGCAGACCAGAGGCCTGAA TCCTCTCTCTTAAACCTGATAACCTACAGAGCCCAGTCTATCCACCCAGCCAAGGATGGCTGGATCCAGA ACCTGCAGTTGTTGATGGAGAGGTTCTTCAGGAACGAGTGCCGCAGTGCCGTGCGCATCAAGGTGCTGGA CGTCCTGTCCTTCGTGCTCCTCATCAACAGGCAGTTCTACGAGGAGGAGCTGATTAACTCGGTGGTCATC TCACAGCTCTCCCACATCCCCGAGGATAAAGACCATCAGGTTCGAAAGCTGGCTACCCAGCTGCTGGTGG ACCTGGCAGAGGGCTGCCACACCCACCACTTCAACAGTCTGTTGGACATCATTGAAAAGGTGATGGCTCG CTCCCTTTCTCCACCCCTGGAGCTGGAGGAAAGGGATCTGGCTGTGTACTCGGCCTCCCTGGAGGATGTG AAGACGGCAGTCCTCGGGCTCCTGGTCATCCTTCAGACCAAGCTGTATACCTTGCCCGCCAGCCATGCCA CACGAGTGTATGAGACACTCATTAGCCACATCCAGCTCCATTACAAGCATGGCTACTCCTTGCCCATTGC TAGCAGCATCCGGCTGCAGGCCTTTGACTTCCTGCTGCTACTGCGGGCTGACTCGCTGCATCGACTGGGC CTGCCCAACAAGGATGGGGTCGTGCGATTCAGCCCTTACTGCCTCTGTGACTGCGCGGAACTGGACAGGG CCTCAGAGAAGAAAGCCAGCGGACCCCTTTCACCGCCAACTGGGCCCCCCAGCCCTGTGCCAACGGGCCC TGCTGTGAGGCTCGGCCACCTGCCCTACTCCCTGCTTTTCCGTGTCCTGCTGCAGTGTTTGAAGCAGGAG ACCGACTGGAAGGTGCTGAAGCTGGTGCTCAGCAAACTCCCTGAGTCGCTGCGCTACAAAGTCCTCATTT TCACCTCCCCCTGCAGTGTTGACCAGCTGTCTTCTGCTCTCTGCTCCATGCTTTCAGCCCCGAAGACCCT TGAGCGGCTCCGAGGCACCCCAGAAGGCTTCTCCAGAACTGACTTGCACTTGGCTGTGGTCCCTGTACTG ACAGCGTTAATATCTTACCACAACTATTTGGATAAGACTAGACAGCGTGAGATGGTTTACTGCCTAGAAC AAGGCCTTATCTACCGCTGTGCCAGCCAGTGTGTGGTAGCCTTGGCCATCTGCAGTGTGGAGATGCCCGA CATCATCATCAAGGCGCTCCCTGTCCTGGTGGTGAAGCTGACACACATCTCTGCTACAGCTAGCATGGCC ATCCCCCTCCTGGAGTTCCTGTCTACCTTGGCCAGGCTGCCTCACCTCTACAGGAACTTCGCCGCAGAGC AGTATGCAAGTGTGTTTGCCATCTCCTTGCCCTACACCAACCCCTCCAAGTTCAACCAGTACATTGTGTG TCTGGCCCATCACGTCATAGCCATGTGGTTCATTAGGTGCCGACTGCCCTTTCGGAAGGATTTTGTCCCT TATATCACTAAGGGTCTGCGTTCCAATGTCCTCCTGTCCTTTGATGATACCCCTGAGAAGGACAAGTTCA GAGCACGGAGCACCAGTCTTAATGAGAGACCCAAAAGTTTGAGGATAGCCAGAGCCCCCAAACAAGGCCT GAATAACTCTCCACCTGTGAAAGAATTCAAAGAGAGCTGTGCAGCCGAGGCCTTCCGGTGCCGCAGCATC AGTGTATCTGAACATGTGGTCCGCAGCAGAATACAGACATCTCTTACCAGTGCCAGCTTGGGGTCTGCGG ATGAGAACTCTATGGCCCAGGCTGATGACAACCTGAAGAATCTCCACTTGGAACTCACAGAAACATGTCT GGACATGATGGCCCGATATGTGTTCTCCAACTTCACTGCGGTTCCCAAGAGGTCCCCTGTGGGAGAATTC CTCCTGGCAGGGGGCAGGACCAAAACGTGGCTGGTTGGAAATAAGCTGGTCACTGTGACAACGAGTGTGG GAACTGGTACGCGGTCGTTGCTGGGCCTGGACTCCGGGGATCTGCAGGGTGGCTCAGCATCAAGCTCTGA TCCTGGCACACATGTGAGGCAGACAAAGGAAGCACCGGCCAAACTGGAGTCCCAGGCTGGGCAGCAGGTG TCCCGTGGGGCCCGGGACCGAGTCCGCTCCATGTCAGGGGGCCACGGCCTTCGAGTTGGTGTCCTGGACA CTTCAGCTCCCTACACCCCAGGAGGCCCTGCTTCTCTGGGAGCACAGGCAGCACCAGCAGCAAGGCCTGA GAAGCCCTGTGCAGGAGCCCAACTTCCGGCAGCGGAGAAGGCGAACCTGGCAGCCTATGTGCCTTTGCTG ACCCAGGGCTGGGCAGAGATCCTGGTCCGCAGGCCCACAGGAAACACCAGCTGGCTGATGAGCTTGGAGA ACCCGCTCAGCCCCTTTTCCTCAGACATCAACAACATGCCCCTGCAAGAGCTGTCTAATGCCCTTATGGC TGCTGAGCGCTTCAAGGAGCACCGGGACACGGCCCTATACAAGTCATTGTCAGTGCCAGCGGCTGGCACA GCCAAGCCTCCTACTCTCCCACGTTCTAACACAGTGGCTTCTTTCTCCTCCCTGTACCAGCCCAGCTGCC AAGGACAGCTGCACAGGAGCGTTTCCTGGGCAGACTCGGCCGTGGTTCTTGAGGAGGGAAGTCCAGGAGA GGCTCATGTGCCAGTGGAGCCCCCCGAATTGGAGGACTTTGAGGCAGCATTAGGTACAGACAGGCACTGC CAGCGGCCTGACGCCTACAGCAGGTCTTCCTCAGCATCTAGCCAGGAAGAAAAGTCCCACTTGGAGGAGC TGGCTGCAGGGGGTATCCCCATTGAGCGGGCCATCTCCTCTGAGGGGGCTCGGCCTACAGTGGACCTGTC CTTCCAACCCTCACAGCCTTTGAGCAAGTCTAGCTCTTCTCCTGAGTTACAGACCTTACAGGACATCCTT GGAGACCTAGGGGACAAGACTGACATTGGACGGCTGAGTCCTGAGGCTAAGGTCCGATCCCAGTCGGGGA TCCTGGATGGGGAAGCAGCTACCTGGTCAGCTCCGGGTGAAGAGAGCCGTATCACAGTCCCACCTGAAGG TCCCCTGCCTTCCAGTTCTCCCCGCTCCCCTAGTGGCCTCCGGCCCCGAGGCTATACCATCTCTGATTCA GCTCCATCACGAAGGGGAAAGAGGGTAGAAAGGGACAACTTCAAGAGCAGAACTGCGGCCTCCAGTGCTG AAAAGGTGCCAGGCATCAACCCTAGCTTTGTGTTCCTACAGCTCTACCATTCACCCTTTTGTGGTGATGA GTCCAATAAGCCCATCCTGTTGCCCAACGAGTCCTTTGAACGGTCAGTGCAGCTCCTTGACCAGATCCCA TCCTATGACACTCACAAGATTGCTGTCCTGTATGTGGGAGAAGGCCAGAGCAGCAGTGAGCTGGCCATCC TATCAAATGAGCATGGCTCTTATAGGTACACAGAGTTTCTGACAGGCCTGGGTCGGCTTATTGAGCTGAA GGACTGCCAACCAGACAAGGTGTACTTAGGTGGACTGGATGTGTGTGGCGAGGATGGACAGTTCACGTAC TGCTGGCATGATGACATCATGCAAGCTGTTTTCCACATTGCTACCCTGATGCCCACCAAGGATGTGGACA AGCACCGTTGTGACAAGAAACGGCACCTGGGCAATGACTTTGTTTCTATCATCTACAATGATTCTGGTGA GGACTTCAAGCTGGGCACCATTAAGGGCCAGTTCAACTTCGTCCATGTGATCATCACACCGCTGGACTAT AAATGCAACCTGTTGACCCTGCAGTGCAGGAAAGACATGGAGGGCCTTGTGGACACCAGTGTGGCCAAGA TTGTGTCTGACCGCAACCTGTCCTTTGTGGCCCGACAGATGGCCCTGCATGCAAATATGGCCTCACAGGT ACACCACAGGCGATCCAACCCCACGGACATCTATCCCTCCAAGTGGATCGCAAGACTCCGCCACATTAAG CGTCTCCGTCAGCGGATCCGTGAAGAAGTGCACTATTCAAACCCAAGCTTGCCTCTGATGCACCCTCCAG CCCATACCAAAGTCCCAGCTCAAGCCCCTACGGAGGCTACACCCACCTACGAGACAGGCCAGCGGAAACG CCTCATTTCCTCTGTGGATGACTTCACAGAGTTTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012680
Insert Size	5430 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012680.3, NP_036812.2
RefSeq Size	5653 bp
RefSeq ORF	5430 bp
Locus ID	24855
UniProt ID	P49816
Gene Summary	acts as a tumor suppressor; may play a role in cell cycle regulation; acute phase response, and negative regulation of cell proliferation [RGD, Feb 2006]

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