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POLR1B (NM_001282772) Human Tagged ORF Clone
CAT＃: RG240022
- TrueORF®
POLR1B (tGFP-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 3
ORF Plasmid: DDK
"NM_001282772" in other vectors (2)
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CNY 12,070.00
货期*
2周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Tag | TurboGFP |
| Synonyms | A135; RPA2; RPA135; Rpo1-2; TCS4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240022 representing NM_001282772.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAGCCAAAGAGGCTGCGGAAACGGGACTGCGGCCACTACTTCCGGCGTGTACCGAGAGACTGGCG TCCGGCGTGTACCGAGAGACTGGCGTCCGGTGTGCAGGTGGCCACATGGATCCTGGCAGCCGGTGGCGG AACCTGCCCAGCGGGCCTAGCCTAAAGCACTTGACTGACCCCTCTTATGGAATCCCGCGGGAACAGCAA AAGGCAGCGTTGCAGGAGCTGACGCGGGCGCACGTGGAGTCCTTCAACTACGCTGTGCACGAGGGTCTC GGCCTCGCGGTGCAGGCTATACCTCCCTTTGAATTTGCTTTCAAAGATGAGCGTATCTCTTTTACTATT CTGGATGCTGTTATCAGTCCACCTACAGTTCCAAAAGGGACCATCTGCAAAGAGGCCAATGTTTATCCA GCAGAATGCCGGGGCCGAAGGAGTACCTACCGTGGGAAGTTGACAGCTGATATCAACTGGGCAGTGAAT GGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGGCTATGTTCCCATCATGGTGAAATCCAAGCTTTGC AACTTACGTAACCTTCCCCCACAAGCCCTCATTGAGCACCATGAGGAGGCAGAGGAAATGGGGGGCTAT TTTATAATCAATGGCATTGAAAAAGTCATCCGAATGTTGATTATGCCTCGGAGAAATTTTCCCATTGCA ATGATAAGACCAAAATGGAAAACCAGAGGGCCTGGTTATACTCAGTATGGAGTTTCAATGCACTGTGTG AGGGAAGAACATTCCGCTGTCAATATGAACCTCCACTACTTGGAAAATGGCACTGTTATGTTGAACTTT ATTTACCGAAAAGAACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAGGCACTTGTCAGCTTTTCTGAT TATCAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCTTTCCTTAGGAACTCTGTTTCTCAG ATGTTAAGGATTGTAATGGAAGAGGGTTGTTCGACACAAAAACAGGTCCTTAACTACCTAGGTGAATGC TTCAGAGTAAAACTCAATGTTCCTGACTGGTACCCAAATGAGCAAGCTGCGGAGTTCCTGTTTAACCAG TGCATCTGTATCCACTTGAAATCCAATACTGAAAAGTTTTATATGCTTTGTCTCATGACGCGAAAGCTC TTTGCTTTAGCCAAAGGAGAGTGCATGGAGGACAATCCTGATAGTTTGGTGAACCAGGAAGTCCTCACA CCGGGTCAGCTCTTCCTTATGTTCCTGAAGGAAAAACTGGAAGGTTGGTTAGTGTCTATTAAAATAGCT TTTGATAAGAAGGCTCAGAAGACCAGTGTTTCCATGAACACTGACAATTTGATGAGGATTTTTACAATG GGCATAGACCTTACAAAACCATTTGAATACCTTTTTGCTACTGGGAATCTGCGTTCTAAAACAGGTCTT GGCCTCCTACAAGATTCTGGACTTTGTGTTGTGGCTGACAAGCTGAACTTCATACGCTACCTCTCCCAT TTCCGCTGCGTGCACAGAGGGGCTGATTTTGCCAAGATGAGGACCACCACAGTACGCAGGCTGCTGCCA GAGTCCTGGGGCTTCCTTTGTCCCGTGCATACCCCAGACGGGGAGCCCTGTGGCCTGATGAACCACCTA ACTGCCGTATGTGAGGTTGTCACACAGTTTGTGTATACGGCATCTATTCCAGCTTTACTGTGCAACTTG GGGGTCACTCCCATTGATGGAGCTCCCCACCGATCATACAGTGAGTGCTACCCTGTCCTGCTGGACGGT GTCATGGTTGGCTGGGTGGATAAGGATCTTGCTCCAGGCATCGCAGATTCTCTTCGTCATTTTAAGGTG TTGAGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTCCTTATACCCATGACAGGAAAACCAAGTCTG TACCCAGGATTGTTCCTTTTTACCACTCCTTGTAGACTGGTACGGCCTGTGCAGAACTTAGCATTGGGC AAAGAAGAGCTAATTGGAACTATGGAACAGATCTTCATGAATGTCGCTATCTTTGAGGATGAAGTTTTT GCTGGAGTTACCACACACCAGGAACTCTTTCCACACAGCCTGCTGAGTGTGATTGCCAACTTCATCCCT TTCTCTGATCACAACCAGAGTCCACGGAACATGTACCAATGCCAGATGGGTAAGCAAACTATGGGCTTT CCACTTCTCACTTATCAAGACCGATCGGATAACAAACTGTATCGTCTTCAGACTCCTCAGAGTCCCTTG GTGAGACCCTCCATGTATGATTATTATGACATGGATAACTATCCAATTGGGACCAATGCCATCGTTGCT GTGATTTCTTACACTGGCTATGATATGGAAGATGCCATGATTGTGAATAAGGCCTCTTGGGAACGAGGC TTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAGACCTCTCTGAAAAAATTAAACAAGGAGATAGT AGCCTGGTGTTTGGCATCAAACCTGGTGACCCACGCGTTCTGCAGAAGTTAGATGACGATGGATTGCCG TTTATAGGAGCAAAACTGCAGTACGGAGATCCGTATTACAGCTACCTCAACCTCAACACCGGGGAAAGT TTTGTGATGTACTATAAGAGTAAAGAAAATTGTGTTGTGGATAACATCAAAGTGTGCAGTAATGACACT GGGAGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGTGCCTCGGAACCCAACTATCGGAGATAAA TTTGCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTGTGGCCGGCTGAGGACATGCCTTTTACT GAGAGTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGTTTTCCATCCCGCATGACCATTGGGATG TTAATTGAGAGTATGGCCGGGAAGTCTGCAGCTTTGCATGGTCTCTGCCATGATGCTACACCCTTCATC TTCTCAGAGGAGAACTCGGCCTTAGAATACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAATTTCTAT GGCACCGAGAGGTTATATAGTGGCATCAGTGGGCTAGAACTGGAAGCAGACATCTTCATAGGAGTGGTT TATTATCAGCGCTTACGCCATATGGTCTCAGACAAATTTCAAGTAAGGACAACTGGAGCCCGAGACAGA GTCACCAACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGTTTTGGGGAGATGGAACGGGAT GCGCTTTTAGCTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTTCAACTGCTCAGATCGGTCGGTA GCCCATGTGTGTGTGAAGTGTGGCAGTTTACTCTCTCCACTGTTGGAGAAGCCACCCCCTTCTTGGTCT GCCATGCGCAACAGAAAATACAACTGTACTCTGTGTAGTCGCAGTGACACTATCGATACTGTTTCTGTG CCTTATGTTTTTCGGTATTTTGTAGCTGAACTGGCAGCTATGAACATCAAAGTGAAACTGGATGTTGTT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240022
Blue=ORF Red=Cloning site Green=Tag(s) MRAKEAAETGLRPLLPACTERLASGVYRETGVRCAGGHMDPGSRWRNLPSGPSLKHLTDPSYGIPREQQ KAALQELTRAHVESFNYAVHEGLGLAVQAIPPFEFAFKDERISFTILDAVISPPTVPKGTICKEANVYP AECRGRRSTYRGKLTADINWAVNGISKGIIKQFLGYVPIMVKSKLCNLRNLPPQALIEHHEEAEEMGGY FIINGIEKVIRMLIMPRRNFPIAMIRPKWKTRGPGYTQYGVSMHCVREEHSAVNMNLHYLENGTVMLNF IYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEDDSFLRNSVSQMLRIVMEEGCSTQKQVLNYLGEC FRVKLNVPDWYPNEQAAEFLFNQCICIHLKSNTEKFYMLCLMTRKLFALAKGECMEDNPDSLVNQEVLT PGQLFLMFLKEKLEGWLVSIKIAFDKKAQKTSVSMNTDNLMRIFTMGIDLTKPFEYLFATGNLRSKTGL GLLQDSGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRRLLPESWGFLCPVHTPDGEPCGLMNHL TAVCEVVTQFVYTASIPALLCNLGVTPIDGAPHRSYSECYPVLLDGVMVGWVDKDLAPGIADSLRHFKV LREKRIPPWMEVVLIPMTGKPSLYPGLFLFTTPCRLVRPVQNLALGKEELIGTMEQIFMNVAIFEDEVF AGVTTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPL VRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDS SLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDT GSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMVPDILFNPHGFPSRMTIGM LIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSGISGLELEADIFIGVV YYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSV AHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTLCSRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVV SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001282772 |
| ORF Size | 3519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001282772.2 |
| RefSeq Size | 5077 bp |
| RefSeq ORF | 3522 bp |
| Locus ID | 84172 |
| UniProt ID | Q9H9Y6 |
| Protein Families | Transcription Factors |
| Protein Pathways | Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| MW | 132.7 kDa |
| Gene Summary | Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008] |
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