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DDX46 (NM_001300860) Human Tagged ORF Clone
CAT＃: RG239901
- TrueORF®
DDX46 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46), transcript variant 1
ORF Plasmid: DDK
"NM_001300860" in other vectors (2)
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CNY 10,640.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Tag | TurboGFP |
| Synonyms | Prp5; PRPF5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239901 representing NM_001300860.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCTGGAAGTCGGTCTAGA AGTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGACGGTCTAGAAGTAGAGATAGAGAT AGGAGGAGAGAGAGGTCTCGTAGCAGGGATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGA CGTTCCAGAAGTAGAGAGAGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGC TCAAGGAGTAGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAG AATAGATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAAGAAAAAAGACAAAGAT GACAAGGAGGATGAAAAAGAAAAAGATGCTGGCAACTTTGACCAGAATAAGCTGGAAGAAGAAATGAGA AAGCGAAAAGAAAGAGTAGAAAAATGGCGAGAAGAGCAACGTAAAAAGGCTATGGAAAACATAGGAGAA CTGAAAAAGGAAATCGAAGAGATGAAACAAGGGAAAAAGTGGAGTTTAGAGGACGATGATGATGACGAA GATGATCCTGCAGAAGCTGAAAAGGAGGGAAATGAAATGGAGGGTGAGGAGTTAGATCCATTAGATGCT TACATGGAAGAAGTGAAAGAGGAAGTAAAAAAATTTAACATGAGAAGTGTAAAAGGTGGTGGGGGAAAT GAAAAGAAGTCTGGGCCAACGGTCACAAAAGTTGTCACTGTTGTGACAACCAAAAAAGCAGTTGTGGAT TCTGATAAGAAGAAAGGTGAGCTGATGGAGAATGACCAGGATGCCATGGAGTATTCTTCAGAGGAGGAA GAAGTTGATCTTCAGACAGCCCTTACAGGGTATCAAACAAAACAGCGAAAGCTTCTAGAACCAGTTGAT CATGGAAAAATTGAGTATGAGCCATTTAGGAAAAACTTCTATGTTGAAGTTCCAGAACTAGCAAAAATG TCTCAAGAAGAGGTAAATGTGTTTCGATTGGAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCC AAACCAATTAAATCCTGGGTCCAGTGTGGAATTTCCATGAAGATCTTAAATTCCCTCAAGAAGCATGGC TATGAAAAGCCCACGCCCATCCAAACCCAAGCTATTCCTGCTATAATGTCTGGACGAGATTTGATTGGC ATTGCCAAAACAGGAAGTGGAAAGACCATTGCTTTTCTGTTGCCCATGTTTAGACACATCATGGATCAG AGGTCATTAGAGGAAGGAGAGGGGCCAATAGCTGTCATCATGACTCCAACTCGAGAACTGGCTTTACAG ATTACTAAAGAGTGTAAGAAGTTTTCCAAGACTTTGGGACTTAGAGTGGTCTGTGTTTACGGAGGAACA GGAATCAGTGAGCAGATTGCTGAGCTGAAAAGAGGTGCTGAAATTATTGTTTGCACACCTGGTCGAATG ATTGACATGTTAGCCGCTAACAGTGGTCGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGAT GAAGCAGACAGAATGTTTGACATGGGTTTTGAACCCCAGGTCATGCGCATCGTGGATAATGTTCGTCCT GATCGACAGACGGTTATGTTTTCAGCTACTTTCCCCAGAGCTATGGAGGCTTTGGCTCGCAGGATCCTC AGTAAACCTATTGAAGTACAAGTTGGAGGCAGGAGTGTGGTTTGCTCAGATGTGGAGCAACAAGTGATT GTGATTGAAGAAGAAAAGAAATTCTTGAAGTTACTTGAGCTTCTAGGCCATTATCAAGAGTCAGGATCT GTCATTATATTTGTGGATAAGCAGGAACATGCTGATGGTCTTCTTAAGGATTTAATGAGAGCATCTTAT CCTTGCATGTCTCTTCATGGAGGCATTGATCAATATGACAGAGATAGCATCATAAATGACTTTAAGAAT GGGACCTGCAAACTTCTTGTGGCTACCTCTGTTGCTGCCCGAGGTCTAGATGTGAAACATCTGATTCTT GTAGTAAATTATAGCTGCCCCAACCATTATGAGGATTATGTACACAGAGCAGGGCGGACTGGAAGAGCA GGAAACAAGGGTTATGCTTATACTTTTATCACAGAGGATCAAGCTCGCTATGCTGGTGACATAATTAAA GCTCTTGAATTGTCAGGGACTGCAGTACCTCCTGATTTAGAGAAACTGTGGAGTGATTTCAAAGATCAG CAGAAAGCTGAGGGGAAAATAATTAAAAAGAGTAGTGGGTTCTCTGGTAAGGGATTCAAGTTTGATGAA ACAGAACAAGCTTTGGCTAATGAGAGGAAGAAGTTACAAAAAGCAGCTCTTGGTCTACAAGATTCAGAT GATGAGGATGCTGCAGTTGATATTGATGAGCAAATTGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAG GATATGGCTGCTCCTGGAACATCAAGTGTTCCTGCTCCAACTGCAGGAAATGCTGAGAAATTAGAAATT GCTAAGAGATTGGCTCTTAGAATCAATGCCCAGAAGAATTTGGGCATCGAGTCTCAGGTAGATGTGATG CAGCAGGCCACCAATGCAATTCTTAGGGGTGGCACCATTCTGGCTCCCACTGTTTCTGCAAAAACCATT GCAGAACAACTTGCTGAAAAGATCAATGCCAAGCTCAATTATGTGCCGTTAGAGAAACAAGAAGAAGAG AGACAGGATGGTGGACAGAATGAATCTTTTAAGAGATATGAAGAAGAATTAGAGATCAATGACTTCCCA CAGACTGCTAGGTGGAAAGTTACCTCTAAGGAAGCTCTGCAGAGAATCAGTGAATACTCTGAAGCCGCA ATTACAATCAGAGGAACCTACTTCCCTCCTGGCAAAGAACCCAAGGAAGGCGAGCGGAAGATTTACTTG GCAATTGAAAGTGCCAATGAACTGGCTGTGCAGAAAGCAAAGGCAGAAATCACCAGGCTCATAAAAGAA GAGCTGATCCGGCTGCAAAATTCATACCAACCAACAAATAAAGGAAGATACAAAGTCTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239901
Blue=ORF Red=Cloning site Green=Tag(s) MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLR RSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKD DKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDE DDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVD SDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKM SQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIG IAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGT GISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRP DRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGS VIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLIL VVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQ QKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVK DMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQVDVMQQATNAILRGGTILAPTVSAKTI AEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAA ITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001300860 |
| ORF Size | 3096 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001300860.2 |
| RefSeq Size | 5750 bp |
| RefSeq ORF | 3099 bp |
| Locus ID | 9879 |
| UniProt ID | Q7L014 |
| Protein Pathways | Spliceosome |
| MW | 117.9 kDa |
| Gene Summary | This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014] |
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