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POLR1B (NM_001282776) Human Tagged ORF Clone
CAT＃: RG239790
- TrueORF®
POLR1B (tGFP-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 5
ORF Plasmid: DDK
"NM_001282776" in other vectors (2)
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CNY 7,510.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Tag | TurboGFP |
| Synonyms | A135; RPA2; RPA135; Rpo1-2; TCS4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239790 representing NM_001282776.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCACTGTGTGAGGGAAGAACATTCCGCTGTCAATATGAACCTCCACTACTTGGAAAATGGCACTGTT ATGTTGAACTTTATTTACCGAAAAGAACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAGGCACTTGTC AGCTTTTCTGATTATCAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCTTTCCTTAGGAAC TCTGTTTCTCAGATGTTAAGGATTGTAATGGAAGAGGGTTGTTCGACACAAAAACAGGTCCTTAACTAC CTAGGTGAATGCTTCAGAGTAAAACTCAATGTTCCTGACTGGTACCCAAATGAGCAAGCTGCGGAGTTC CTGTTTAACCAGTGCATCTGTATCCACTTGAAATCCAATACTGAAAAGTTTTATATGCTTTGTCTCATG ACGCGAAAGCTCTTTGCTTTAGCCAAAGGAGAGTGCATGGAGGACAATCCTGATAGTTTGGTGAACCAG GAAGTCCTCACACCGGGTCAGCTCTTCCTTATGTTCCTGAAGGAAAAACTGGAAGGTTGGTTAGTGTCT ATTAAAATAGCTTTTGATAAGAAGGCTCAGAAGACCAGTGTTTCCATGAACACTGACAATTTGATGAGG ATTTTTACAATGGGCATAGACCTTACAAAACCATTTGAATACCTTTTTGCTACTGGGAATCTGCGTTCT AAAACAGGTCTTGGCCTCCTACAAGATTCTGGACTTTGTGTTGTGGCTGACAAGCTGAACTTCATACGC TACCTCTCCCATTTCCGCTGCGTGCACAGAGGGGCTGATTTTGCCAAGATGAGGACCACCACAGTACGC AGGCTGCTGCCAGAGTCCTGGGGCTTCCTTTGTCCCGTGCATACCCCAGACGGGGAGCCCTGTGGCCTG ATGAACCACCTAACTGCCGTATGTGAGGTTGTCACACAGTTTGTGTATACGGCATCTATTCCAGCTTTA CTGTGCAACTTGGGGGTCACTCCCATTGATGGAGCTCCCCACCGATCATACAGTGAGTGCTACCCTGTC CTGCTGGACGGTGTCATGGTTGGCTGGGTGGATAAGGATCTTGCTCCAGGCATCGCAGATTCTCTTCGT CATTTTAAGGTGTTGAGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTCCTTATACCCATGACAGGA AAACCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTCCTTGTAGACTGGTACGGCCTGTGCAGAAC TTAGCATTGGGCAAAGAAGAGCTAATTGGAACTATGGAACAGATCTTCATGAATGTCGCTATCTTTGAG GATGAAGTTTTTGCTGGAGTTACCACACACCAGGAACTCTTTCCACACAGCCTGCTGAGTGTGATTGCC AACTTCATCCCTTTCTCTGATCACAACCAGAGTCCACGGAACATGTACCAATGCCAGATGGGTAAGCAA ACTATGGGCTTTCCACTTCTCACTTATCAAGACCGATCGGATAACAAACTGTATCGTCTTCAGACTCCT CAGAGTCCCTTGGTGAGACCCTCCATGTATGATTATTATGACATGGATAACTATCCAATTGGGACCAAT GCCATCGTTGCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCCATGATTGTGAATAAGGCCTCT TGGGAACGAGGCTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAGACCTCTCTGAAAAAATTAAA CAAGGAGATAGTAGCCTGGTGTTTGGCATCAAACCTGGTGACCCACGCGTTCTGCAGAAGTTAGATGAC GATGGATTGCCGTTTATAGGAGCAAAACTGCAGTACGGAGATCCGTATTACAGCTACCTCAACCTCAAC ACCGGGGAAAGTTTTGTGATGTACTATAAGAGTAAAGAAAATTGTGTTGTGGATAACATCAAAGTGTGC AGTAATGACACTGGGAGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGTGCCTCGGAACCCAACT ATCGGAGATAAATTTGCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTGTGGCCGGCTGAGGAC ATGCCTTTTACTGAGAGTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGTTTTCCATCCCGCATG ACCATTGGGATGTTAATTGAGAGTATGGCCGGGAAGTCTGCAGCTTTGCATGGTCTCTGCCATGATGCT ACACCCTTCATCTTCTCAGAGGAGAACTCGGCCTTAGAATACTTTGGTGAGATGTTAAAGGCTGCTGGC TACAATTTCTATGGCACCGAGAGGTTATATAGTGGCATCAGTGGGCTAGAACTGGAAGCAGACATCTTC ATAGGAGTGGTTTATTATCAGCGCTTACGCCATATGGTCTCAGACAAATTTCAAGTAAGGACAACTGGA GCCCGAGACAGAGTCACCAACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGTTTTGGGGAG ATGGAACGGGATGCGCTTTTAGCTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTTCAACTGCTCA GATCGGTCGGTAGCCCATGTGTGTGTGAAGTGTGGCAGTTTACTCTCTCCACTGTTGGAGAAGCCACCC CCTTCTTGGTCTGCCATGCGCAACAGAAAATACAACTGTACTCTGTGTAGTCGCAGTGACACTATCGAT ACTGTTTCTGTGCCTTATGTTTTTCGGTATTTTGTAGCTGAACTGGCAGCTATGAACATCAAAGTGAAA CTGGATGTTGTT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239790
Blue=ORF Red=Cloning site Green=Tag(s) MHCVREEHSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEDDSFLRN SVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQAAEFLFNQCICIHLKSNTEKFYMLCLM TRKLFALAKGECMEDNPDSLVNQEVLTPGQLFLMFLKEKLEGWLVSIKIAFDKKAQKTSVSMNTDNLMR IFTMGIDLTKPFEYLFATGNLRSKTGLGLLQDSGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVR RLLPESWGFLCPVHTPDGEPCGLMNHLTAVCEVVTQFVYTASIPALLCNLGVTPIDGAPHRSYSECYPV LLDGVMVGWVDKDLAPGIADSLRHFKVLREKRIPPWMEVVLIPMTGKPSLYPGLFLFTTPCRLVRPVQN LALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQ TMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKAS WERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYYSYLNLN TGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAED MPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAG YNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGE MERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTLCSRSDTID TVSVPYVFRYFVAELAAMNIKVKLDVV SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001282776 |
| ORF Size | 2772 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001282776.2 |
| RefSeq Size | 4979 bp |
| RefSeq ORF | 2775 bp |
| Locus ID | 84172 |
| UniProt ID | Q9H9Y6 |
| Protein Families | Transcription Factors |
| Protein Pathways | Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| MW | 104.9 kDa |
| Gene Summary | Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008] |
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