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l Afadin (AFDN) (NM_001207008) Human Tagged ORF Clone
CAT＃: RG235333
- TrueORF®
MLLT4 (tGFP-tagged) - Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 (MLLT4), transcript variant 1
ORF Plasmid: DDK
"NM_001207008" in other vectors (2)
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CNY 19,100.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AF6; l-afadin; MLL-AF6; MLLT4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235333 representing NM_001207008
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGGCGGCCGTGACGAGGAGCGGCGGAAGCTGGCCGACATCATCCACCACTGGAACGCCAACC GGCTGGACCTGTTCGAGATCAGCCAGCCGACCGAGGATTTGGAGTTCCATGGAGTGATGAGATTTTATTT TCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTCTCTAGTACTGCCACCACTCAAGAT GTAATCGAAACGCTCGCGGAGAAATTTCGACCTGATATGCGAATGCTGTCCTCTCCCAAGTATTCACTCT ATGAAGTGCATGTCAGCGGAGAAAGAAGATTGGATATAGATGAGAAACCTCTAGTTGTACAACTGAATTG GAACAAAGATGATCGGGAAGGCAGATTTGTTCTTAAGAATGAGAATGACGCCATTCCTCCTAAGGCTCAA AGTAATGGACCTGAAAAGCAGGAAAAAGAAGGGGTTATCCAGAACTTCAAGAGAACTCTCTCAAAGAAAG AAAAGAAGGAAAAAAAGAAGAGAGAAAAAGAGGCATTGCGACAGGCATCTGATAAAGATGATAGACCTTT CCAAGGGGAGGATGTTGAAAATTCTCGACTGGCTGCTGAGGTTTACAAAGACATGCCGGAAACCAGCTTT ACTCGAACCATTTCTAATCCTGAGGTGGTTATGAAACGACGGAGGCAGCAAAAATTGGAAAAGAGAATGC AGGAATTTCGGAGCTCAGATGGGCGGCCTGATTCAGGTGGAACATTGAGAATTTATGCAGATAGTTTAAA ACCAAATATTCCCTACAAGACAATCCTGCTGTCTACTACAGATCCTGCAGACTTTGCTGTGGCTGAAGCT TTAGAGAAGTATGGTCTGGAAAAAGAAAACCCTAAGGATTACTGCATCGCCCGGGTTATGCTTCCTCCTG GAGCCCAGCATTCTGATGAAAAGGGTGCTAAAGAAATTATTCTTGATGATGATGAGTGTCCTTTACAAAT CTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGGCCACCAGACCACATC CCAAAGAAAACCAAGAAACACTTGGAAGGCAAGACACCCAAGGGAAAGGAGAGAGCTGACGGGTCTGGCT ATGGCTCCACCCTTCCTCCGGAGAAGCTGCCCTATTTAGTAGAGTTAAGCCCAGATGGTTCTGACTCTAG AGATAAGCCAAAGCTTTACCGCCTTCAGTTAAGTGTTACTGAAGTTGGGACAGAAAAGTTGGATGACAAC TCTATCCAGTTGTTTGGCCCAGGAATTCAGCCCCATCACTGTGACCTTACCAACATGGATGGAGTGGTCA CTGTGACGCCCAGAAGTATGGACGCAGAAACCTACGTGGAAGGCCAGCGCATCTCAGAAACCACCATGCT GCAGAGTGGCATGAAAGTGCAGTTTGGGGCGTCCCATGTATTTAAGTTTGTGGACCCCAGTCAGGATCAT GCTCTTGCAAAAAGATCTGTGGATGGAGGCCTGATGGTTAAGGGCCCAAGACATAAACCTGGAATTGTTC AGGAGACAACTTTTGATTTGGGAGGAGATATTCATAGTGGGACAGCATTACCGACAAGCAAGAGCACCAC TAGGCTGGACAGCGACAGAGTGTCGTCTGCCTCTAGCACAGCCGAGCGGGGAATGGTGAAGCCGATGATC AGAGTAGAACAGCAGCCAGATTATCGCAGGCAAGAAAGCAGAACACAGGATGCTTCTGGGCCTGAGCTGA TACTACCTGCAAGCATTGAATTCAGGGAAAGTTCTGAAGATTCATTTTTGTCTGCCATTATAAATTATAC TAATAGCTCTACAGTCCACTTTAAGTTGTCCCCTACATATGTATTATATATGGCATGCCGGTATGTATTG TCCAACCAGTACAGACCTGACATCAGCCCTACAGAGCGCACACATAAAGTCATTGCAGTCGTCAACAAGA TGGTGAGCATGATGGAGGGTGTCATCCAGAAACAGAAGAATATTGCAGGGGCACTTGCCTTCTGGATGGC AAATGCATCTGAACTTCTCAACTTCATTAAGCAAGACCGAGACCTTAGTCGGATCACACTGGATGCTCAA GATGTTTTAGCACATTTGGTTCAAATGGCATTTAAATACTTGGTTCACTGTCTTCAATCAGAACTTAATA ATTACATGCCAGCCTTTCTAGATGACCCTGAAGAGAACAGTCTGCAACGACCAAAAATAGATGATGTGCT GCACACGCTCACAGGAGCCATGTCCTTGCTACGACGCTGCAGAGTCAATGCCGCCCTGACCATCCAGCTC TTCTCTCAGCTCTTCCACTTCATCAATATGTGGCTGTTCAATAGATTGGTGACCGACCCAGATTCGGGGC TGTGCTCCCATTACTGGGGTGCGATTATCCGTCAGCAGTTGGGCCATATTGAAGCCTGGGCTGAGAAGCA GGGGCTGGAACTGGCTGCGGACTGTCATCTGAGCAGGATCGTGCAGGCAACGACTTTGCTTACCATGGAT AAGTATGCACCTGATGACATTCCAAATATAAACAGCACCTGCTTTAAGTTAAATTCATTACAACTTCAAG CCTTATTACAGAACTATCACTGTGCACCTGATGAGCCTTTTATCCCAACGGATCTTATAGAAAATGTAGT GACTGTGGCTGAAAACACTGCCGATGAGCTGGCCCGCAGTGATGGAAGGGAAGTGCAGTTGGAGGAGGAT CCTGATCTGCAGCTGCCGTTTCTTTTGCCAGAAGATGGTTATTCTTGTGATGTTGTCAGAAACATTCCAA ATGGTTTACAAGAATTTTTAGACCCTCTGTGCCAGAGAGGATTTTGCAGGTTAATTCCTCACACACGTTC ACCAGGTACTTGGACAATATATTTTGAAGGTGCAGATTATGAAAGTCACCTTCTGCGTGAGAACACAGAG CTGGCTCAGCCTCTGAGGAAAGAACCTGAAATAATCACTGTGACCCTAAAAAAGCAGAATGGAATGGGCC TTAGCATTGTTGCAGCAAAGGGTGCTGGTCAAGATAAACTAGGAATCTATGTGAAGTCGGTTGTGAAAGG AGGTGCTGCAGATGATGGACGTCTAGCTGCAGGTGATCAGCTCCTCAGTGTGGATGGACGAAGTCTGGTT GGACTCTCTCAGGAAAGGGCGGCAGAACTCATGACAAGAACAAGCTCTGTGGTGACACTGGAAGTAGCAA AGCAGGGTGCCATCTACCACGGTCTGGCCACCCTTCTCAATCAGCCATCCCCCATGATGCAGAGAATTTC AGATCGTCGTGGCTCAGGTAAACCCCGACCAAAGAGTGAAGGCTTTGAGCTCTATAATAATTCAACTCAA AATGGGTCTCCTGAGAGTCCTCAGCTGCCTTGGGCAGAATATAGTGAACCAAAGAAATTGCCTGGTGATG ACAGACTGATGAAAAATAGAGCTGATCACCGTTCCAGCCCCAACGTAGCAAATCAGCCTCCTAGTCCTGG AGGGAAAAGTGCATATGCCTCTGGAACAACAGCGAAGATAACATCTGTCTCTACTGGAAACCTCTGCACT GAGGAGCAGACGCCTCCGCCTAGACCTGAAGCCTACCCCATCCCCACTCAGACGTACACCAGAGAGTATT TTACCTTCCCAGCTTCCAAATCCCAGGATCGGATGGCTCCTCCTCAGAACCAGTGGCCAAATTATGAGGA AAAGCCACATATGCACACAGATAGTAATCATTCCAGTATTGCAATTCAGCGTGTTACACGTTCCCAAGAA GAACTTCGAGAAGATAAAGCTTACCAACTTGAGCGGCATCGAATAGAGGCAGCTATGGACCGAAAGTCTG ATAGTGATATGTGGATAAATCAGAGCTCCTCACTGGACTCCAGTACCTCTAGCCAGGAGCATCTGAACCA TTCCTCTAAGTCGGTCACCCCTGCTTCCACACTGACCAAAAGTGGCCCTGGCCGTTGGAAAACACCAGCA GCCATACCGGCCACCCCTGTGGCCGTCTCCCAGCCAATCCGAACAGACCTGCCTCCGCCACCCCCGCCAC CTCCAGTCCACTATGCCGGTGATTTCGATGGAATGTCCATGGATTTGCCTCTCCCACCACCCCCTTCCGC CAACCAGATAGGGCTGCCGTCTGCGCAGGTGGCTGCTGCTGAACGGAGAAAGAGAGAAGAACATCAGCGT TGGTATGAGAAGGAGAAGGCCCGCCTGGAGGAGGAGCGGGAGAGGAAGCGGAGAGAGCAGGAGAGGAAGT TGGGCCAGATGCGCACTCAGTCCTTAAACCCTGCTCCGTTTTCTCCCCTGACTGCACAGCAGATGAAGCC CGAAAAGCCTTCCACACTCCAGCGGCCACAGGAAACAGTCATTCGGGAGCTGCAGCCTCAGCAGCAGCCC CGCACGATCGAGCGCAGAGACTTGCAGTACATTACAGTCAGCAAAGAGGAGCTTTCCTCGGGGGACAGTC TGTCCCCCGACCCGTGGAAGCGGGACGCCAAGGAGAAGCTGGAGAAGCAGCAGCAGATGCACATCGTGGA CATGCTGAGCAAGGAGATCCAGGAGCTCCAGAGCAAACCGGACCGCAGCGCCGAGGAGAGCGACCGGCTG CGCAAGCTCATGCTGGAGTGGCAGTTCCAGAAGAGACTCCAGGAGTCGAAGCAGAAGGACGAAGATGACG AGGAGGAGGAGGACGATGATGTGGACACCATGCTGATCATGCAGCGCCTGGAGGCTGAACGAAGAGCGAG GTTGCAGGACGAGGAGCGGAGGCGGCAGCAGCAGTTAGAAGAGATGCGCAAGCGGGAAGCGGAAGACCGA GCGAGGCAAGAGGAAGAGCGCCGGCGGCAGGAGGAGGAGCGAACAAAACGAGACGCTGAAGAAAAGAGGC GACAGGAAGAAGGGTATTACAGCCGCCTGGAAGCCGAGAGGCGCAGACAGCACGACGAGGCGGCGCGCAG GTTGCTGGAGCCCGAGGCGCCCGGACCAAACTCTTACCCAGGATCTACTGGAGCAGCTGTTGGAGCCCAT GACGCCTGTCGGGATGCAAAAGAGAAGCGCTCTAAAAGCCAAGATGCAGATTCACCTGGAAGTTCTGGGG CCCCTGAAAACTTGACATTCAAGGAACGCCAGCGTCTTTTTTCACAAGGTCAAGATGTATCCAATAAAGT GAAAGCTTCTCGTAAATTAACAGAACTGGAGAATGAACTGAACACAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235333 representing NM_001207008
Red=Cloning site Green=Tags(s) MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQD VIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQ SNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSF TRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEA LEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHI PKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDN SIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDH ALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMI RVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVL SNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQ DVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQL FSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMD KYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEED PDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTE LAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADDGRLAAGDQLLSVDGRSLV GLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQ NGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCT EEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQE ELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPA AIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQR WYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQP RTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRL RKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDR ARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAH DACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001207008 |
| ORF Size | 5229 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001207008.1, NP_001193937.1 |
| RefSeq Size | 7459 bp |
| RefSeq ORF | 5232 bp |
| Locus ID | 4301 |
| UniProt ID | P55196 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Leukocyte transendothelial migration, Tight junction |
| Gene Summary | This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC235333 | MLLT4 (Myc-DDK tagged) - Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 4 (MLLT4), transcript variant 1 |
CNY 17,390.00 |
|
| SC331731 | MLLT4 (untagged) - Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 4 (MLLT4), transcript variant 1 |
CNY 22,330.00 |
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