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TRPM1 (NM_001252024) Human Tagged ORF Clone
CAT＃: RG235323
- TrueORF®
TRPM1 (tGFP-tagged) - Homo sapiens transient receptor potential cation channel, subfamily M, member 1 (TRPM1), transcript variant 3
ORF Plasmid: DDK
"NM_001252024" in other vectors (2)
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CNY 17,860.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CSNB1C; LTRPC1; MLSN1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235323 representing NM_001252024
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCAGAAATCTTGGATAGAGAAAACCTTTTGCAAACGGGAATGTATCTTTGTAATTCCTAGCATGA AAGACTCTAACAGGTGTTGCTGTGGCCAGTTCACCAACCAGCATATCCCCCCTCTGCCAAGTGCAACACC CAGCAAAAATGAAGAGGAAAGCAAACAGGTGGAGACTCAGCCTGAGAAATGGTCTGTTGCCAAGCACACC CAGAGCTACCCAACAGATTCCTATGGAGTTCTTGAATTCCAGGGTGGCGGATATTCCAATAAAGCCATGT ATATCCGTGTATCCTATGACACCAAGCCAGACTCACTGCTCCATCTCATGGTGAAAGATTGGCAGCTGGA ACTCCCCAAGCTCTTAATATCTGTGCATGGAGGCCTCCAGAACTTTGAGATGCAGCCCAAGCTGAAACAA GTCTTTGGGAAAGGCCTGATCAAGGCTGCTATGACCACCGGGGCCTGGATCTTCACCGGGGGTGTCAGCA CAGGTGTTATCAGCCACGTAGGGGATGCCTTGAAAGACCACTCCTCCAAGTCCAGAGGCCGGGTTTGTGC TATAGGAATTGCTCCATGGGGCATCGTGGAGAATAAGGAAGACCTGGTTGGAAAGGATGTAACAAGAGTG TACCAGACCATGTCCAACCCTCTAAGTAAGCTCTCTGTGCTCAACAACTCCCACACCCACTTCATCCTGG CTGACAATGGCACCCTGGGCAAGTATGGCGCCGAGGTGAAGCTGCGAAGGCTGCTGGAAAAGCACATCTC CCTGCAGAAGATCAACACAAGACTGGGGCAGGGCGTGCCCCTCGTGGGTCTCGTGGTGGAGGGGGGCCCT AACGTGGTGTCCATCGTCTTGGAATACCTGCAAGAAGAGCCTCCCATCCCTGTGGTGATTTGTGATGGCA GCGGACGTGCCTCGGACATCCTGTCCTTTGCGCACAAGTACTGTGAAGAAGGCGGAATAATAAATGAGTC CCTCAGGGAGCAGCTTCTAGTTACCATTCAGAAAACATTTAATTATAATAAGGCACAATCACATCAGCTG TTTGCAATTATAATGGAGTGCATGAAGAAGAAAGAACTCGTCACTGTGTTCAGAATGGGTTCTGAGGGCC AGCAGGACATCGAGATGGCAATTTTAACTGCCCTGCTGAAAGGAACAAACGTATCTGCTCCAGATCAGCT GAGCTTGGCACTGGCTTGGAACCGCGTGGACATAGCACGAAGCCAGATCTTTGTCTTTGGGCCCCACTGG CCGCCCCTGGGAAGCCTGGCACCCCCGACGGACAGCAAAGCCACGGAGAAGGAGAAGAAGCCACCCATGG CCACCACCAAGGGAGGAAGAGGAAAAGGGAAAGGCAAGAAGAAAGGGAAAGTGAAAGAGGAAGTGGAGGA AGAAACTGACCCCCGGAAGATAGAGCTGCTGAACTGGGTGAATGCTTTGGAGCAAGCGATGCTAGATGCT TTAGTCTTAGATCGTGTCGACTTTGTGAAGCTCCTGATTGAAAACGGAGTGAACATGCAACACTTTCTGA CCATTCCGAGGCTGGAGGAGCTTTATAACACAAGACTGGGTCCACCAAACACACTTCATCTGCTGGTGAG GGATGTGAAAAAGAGCAACCTTCCGCCTGATTACCACATCAGCCTCATAGACATCGGGCTCGTGCTGGAG TACCTCATGGGAGGAGCCTACCGCTGCAACTACACTCGGAAAAACTTTCGGACCCTTTACAACAACTTGT TTGGACCAAAGAGGCCTAAAGCTCTTAAACTTCTGGGAATGGAAGATGATGAGCCTCCAGCTAAAGGGAA GAAAAAGAAAAAGAAGAAAAAGGAGGAAGAGATCGACATTGATGTGGACGACCCTGCCGTGAGTCGGTTC CAGTATCCCTTCCACGAGCTGATGGTGTGGGCAGTGCTGATGAAACGCCAGAAAATGGCAGTGTTCCTCT GGCAGCGAGGGGAAGAGAGCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTACAAGGCCATGGCCCACGA GTCCTCCGAGAGTGATCTGGTGGATGACATCTCCCAGGACTTGGATAACAATTCCAAAGACTTCGGCCAG CTTGCTTTGGAGTTATTAGACCAGTCCTATAAGCATGACGAGCAGATCGCTATGAAACTCCTGACCTACG AGCTGAAAAACTGGAGCAACTCGACCTGCCTCAAACTGGCCGTGGCAGCCAAACACCGGGACTTCATTGC TCACACCTGCAGCCAGATGCTGCTGACCGATATGTGGATGGGAAGACTGCGGATGCGGAAGAACCCCGGC CTGAAGGTTATCATGGGGATTCTTCTACCCCCCACCATCTTGTTTTTGGAATTTCGCACATATGATGATT TCTCGTATCAAACATCCAAGGAAAATGAGGATGGCAAAGAAAAAGAAGAGGAAAATACGGATGCAAATGC AGATGCTGGCTCAAGAAAGGGGGATGAGGAGAACGAGCACAAAAAACAGAGAAGTATTCCCATCGGAACA AAGATCTGTGAATTCTATAACGCGCCCATTGTCAAGTTCTGGTTTTACACAATATCATACTTGGGCTACC TGCTGCTGTTTAACTACGTCATCCTGGTGCGGATGGATGGCTGGCCGTCCCTCCAGGAGTGGATCGTCAT CTCCTACATCGTGAGCCTGGCGTTAGAGAAGATACGAGAGATCCTCATGTCAGAACCAGGCAAACTCAGC CAGAAAATCAAAGTTTGGCTTCAGGAGTACTGGAACATCACAGATCTCGTGGCCATTTCCACATTCATGA TTGGAGCAATTCTTCGCCTACAGAACCAGCCCTACATGGGCTATGGCCGGGTGATCTACTGTGTGGATAT CATCTTCTGGTACATCCGTGTCCTGGACATCTTTGGTGTCAACAAGTATCTGGGGCCATACGTGATGATG ATTGGAAAGATGATGATCGACATGCTGTACTTTGTGGTCATCATGCTGGTCGTGCTCATGAGTTTCGGAG TAGCCCGTCAAGCCATTCTGCATCCAGAGGAGAAGCCCTCTTGGAAACTGGCCCGAAACATCTTCTACAT GCCCTACTGGATGATCTATGGAGAGGTGTTTGCAGACCAGATAGACCTCTACGCCATGGAAATTAATCCT CCTTGTGGTGAGAACCTATATGATGAGGAGGGCAAGCGGCTTCCTCCCTGTATCCCCGGCGCCTGGCTCA CTCCAGCACTCATGGCGTGCTATCTACTGGTCGCCAACATCCTGCTGGTGAACCTGCTGATTGCTGTGTT CAACAATACCTTCTTTGAAGTAAAATCAATATCCAACCAGGTGTGGAAGTTCCAGCGATATCAGCTGATT ATGACATTTCATGACAGGCCAGTCCTGCCCCCACCGATGATCATTTTAAGCCACATCTACATCATCATTA TGCGTCTCAGCGGCCGCTGCAGGAAAAAGAGAGAAGGGGACCAAGAGGAACGGGATCGTGGATTGAAGCT CTTCCTTAGCGACGAGGAGCTAAAGAGGCTGCATGAGTTCGAGGAGCAGTGCGTGCAGGAGCACTTCCGG GAGAAGGAGGATGAGCAGCAGTCGTCCAGCGACGAGCGCATCCGGGTCACTTCTGAAAGAGTTGAAAATA TGTCAATGAGGTTGGAAGAAATCAATGAAAGAGAAACTTTTATGAAAACTTCCCTGCAGACTGTTGACCT TCGACTTGCTCAGCTAGAAGAATTATCTAACAGAATGGTGAATGCTCTTGAAAATCTTGCGGGAATCGAC AGGTCTGACCTGATCCAGGCACGGTCCCGGGCTTCTTCTGAATGTGAGGCAACGTATCTTCTCCGGCAAA GCAGCATCAATAGCGCTGATGGCTACAGCTTGTATCGATATCATTTTAACGGAGAAGAGTTATTATTTGA GGATACATCTCTCTCCACGTCACCAGGGACAGGAGTCAGGAAAAAAACCTGTTCCTTCCGTATAAAGGAA GAGAAGGACGTGAAAACGCACCTAGTCCCAGAATGTCAGAACAGTCTTCACCTTTCACTGGGCACAAGCA CATCAGCAACCCCAGATGGCAGTCACCTTGCAGTAGATGACTTAAAGAACGCTGAAGAGTCAAAATTAGG TCCAGATATTGGGATTTCAAAGGAAGATGATGAAAGACAGACAGACTCTAAAAAAGAAGAAACTATTTCC CCAAGTTTAAATAAAACAGATGTGATACATGGACAGGACAAATCAGATGTTCAAAACACTCAGCTAACAG TGGAAACGACAAATATAGAAGGCACTATTTCCTATCCCCTGGAAGAAACCAAAATTACACGCTATTTCCC CGATGAAACGATCAATGCTTGTAAAACAATGAAGTCCAGAAGCTTCGTCTATTCCCGGGGAAGAAAGCTG GTCGGTGGGGTTAACCAGGATGTAGAGTACAGTTCAATCACGGACCAGCAATTGACGACGGAATGGCAAT GCCAAGTTCAAAAGATCACGCGCTCTCATAGCACAGATATTCCTTACATTGTGTCGGAAGCTGCAGTGCA AGCTGAGCATAAAGAGCAGTTTGCAGATATGCAAGATGAACACCATGTCGCTGAAGCAATTCCTCGAATC CCTCGCTTGTCCCTAACCATTACTGACAGAAATGGGATGGAAAACTTACTGTCTGTGAAGCCAGATCAAA CTTTGGGATTCCCATCTCTCAGGTCAAAAAGTTTACATGGACATCCTAGGAATGTGAAATCCATTCAGGG AAAGTTAGACAGATCTGGACATGCCAGTAGTGTAAGCAGCTTAGTAATTGTGTCTGGAATGACAGCAGAA GAAAAAAAGGTTAAGAAAGAGAAAGCTTCCACAGAAACTGAATGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235323 representing NM_001252024
Red=Cloning site Green=Tags(s) MGQKSWIEKTFCKRECIFVIPSMKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHT QSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQ VFGKGLIKAAMTTGAWIFTGGVSTGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRV YQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGP NVVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTFNYNKAQSHQL FAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSLALAWNRVDIARSQIFVFGPHW PPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKKGKVKEEVEEETDPRKIELLNWVNALEQAMLDA LVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLE YLMGGAYRCNYTRKNFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRF QYPFHELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNNSKDFGQ LALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPG LKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENTDANADAGSRKGDEENEHKKQRSIPIGT KICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLS QKIKVWLQEYWNITDLVAISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMM IGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINP PCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLI MTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFR EKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGID RSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKE EKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETIS PSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKL VGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRI PRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAE EKKVKKEKASTETEC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001252024 |
| ORF Size | 4875 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001252024.2 |
| RefSeq Size | 5810 bp |
| RefSeq ORF | 4878 bp |
| Locus ID | 4308 |
| UniProt ID | Q7Z4N2 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| Gene Summary | This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC235323 | TRPM1 (Myc-DDK tagged) - Homo sapiens transient receptor potential cation channel, subfamily M, member 1 (TRPM1), transcript variant 3 |
CNY 16,250.00 |
|
| SC332165 | TRPM1 (untagged) - Homo sapiens transient receptor potential cation channel, subfamily M, member 1 (TRPM1), transcript variant 3 |
CNY 15,580.00 |
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