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NOS1 (NM_001204218) Human Tagged ORF Clone
CAT＃: RG235311
- TrueORF®
NOS1 (tGFP-tagged) - Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 2
ORF Plasmid: DDK
"NM_001204218" in other vectors (2)
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CNY 16,060.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235311 representing NM_001204218
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATCACATGTTCGGTGTTCAGCAAATCCAGCCCAATGTCATTTCTGTTCGTCTCTTCAAGCGCA AAGTTGGGGGCCTGGGATTTCTGGTGAAGGAGCGGGTCAGTAAGCCGCCCGTGATCATCTCTGACCTGAT TCGTGGGGGCGCCGCAGAGCAGAGTGGCCTCATCCAGGCCGGAGACATCATTCTTGCGGTCAACGGCCGG CCCTTGGTGGACCTGAGCTATGACAGCGCCCTGGAGGTACTCAGAGGCATTGCCTCTGAGACCCACGTGG TCCTCATTCTGAGGGGCCCTGAAGGTTTCACCACGCACCTGGAGACCACCTTTACAGGTGATGGGACCCC CAAGACCATCCGGGTGACACAGCCCCTGGGTCCCCCCACCAAAGCCGTGGATCTGTCCCACCAGCCACCG GCCGGCAAAGAACAGCCCCTGGCAGTGGATGGGGCCTCGGGTCCCGGGAATGGGCCTCAGCATGCCTACG ATGATGGGCAGGAGGCTGGCTCACTCCCCCATGCCAACGGCCTGGCCCCCAGGCCCCCAGGCCAGGACCC CGCGAAGAAAGCAACCAGAGTCAGCCTCCAAGGCAGAGGGGAGAACAATGAACTGCTCAAGGAGATAGAG CCTGTGCTGAGCCTTCTCACCAGTGGGAGCAGAGGGGTCAAGGGAGGGGCACCTGCCAAGGCAGAGATGA AAGATATGGGAATCCAGGTGGACAGAGATTTGGACGGCAAGTCACACAAACCTCTGCCCCTCGGCGTGGA GAACGACCGAGTCTTCAATGACCTATGGGGGAAGGGCAATGTGCCTGTCGTCCTCAACAACCCATATTCA GAGAAGGAGCAGCCCCCCACCTCAGGAAAACAGTCCCCCACAAAGAATGGCAGCCCCTCCAAGTGTCCAC GCTTCCTCAAGGTCAAGAACTGGGAGACTGAGGTGGTTCTCACTGACACCCTCCACCTTAAGAGCACATT GGAAACGGGATGCACTGAGTACATCTGCATGGGCTCCATCATGCATCCTTCTCAGCATGCAAGGAGGCCT GAAGACGTCCGCACAAAAGGACAGCTCTTCCCTCTCGCCAAAGAGTTTATTGATCAATACTATTCATCAA TTAAAAGATTTGGCTCCAAAGCCCACATGGAAAGGCTGGAAGAGGTGAACAAAGAGATCGACACCACTAG CACTTACCAGCTCAAGGACACAGAGCTCATCTATGGGGCCAAGCACGCCTGGCGGAATGCCTCGCGCTGT GTGGGCAGGATCCAGTGGTCCAAGCTGCAGGTATTCGATGCCCGTGACTGCACCACGGCCCACGGGATGT TCAACTACATCTGTAACCATGTCAAGTATGCCACCAACAAAGGGAACCTCAGGTCTGCCATCACCATATT CCCCCAGAGGACAGACGGCAAGCACGACTTCCGAGTCTGGAACTCCCAGCTCATCCGCTACGCTGGCTAC AAGCAGCCTGACGGCTCCACCCTGGGGGACCCAGCCAATGTGCAGTTCACAGAGATATGCATACAGCAGG GCTGGAAACCGCCTAGAGGCCGCTTCGATGTCCTGCCGCTCCTGCTTCAGGCCAACGGCAATGACCCTGA GCTCTTCCAGATTCCTCCAGAGCTGGTGTTGGAAGTTCCCATCAGGCACCCCAAGTTTGAGTGGTTCAAG GACCTGGGGCTGAAGTGGTACGGCCTCCCCGCCGTGTCCAACATGCTCCTAGAGATTGGCGGCCTGGAGT TCAGCGCCTGTCCCTTCAGTGGCTGGTACATGGGCACAGAGATTGGTGTCCGCGACTACTGTGACAACTC CCGCTACAATATCCTGGAGGAAGTGGCCAAGAAGATGAACTTAGACATGAGGAAGACGTCCTCCCTGTGG AAGGACCAGGCGCTGGTGGAGATCAATATCGCGGTTCTCTATAGCTTCCAGAGTGACAAAGTGACCATTG TTGACCATCACTCCGCCACCGAGTCCTTCATTAAGCACATGGAGAATGAGTACCGCTGCCGGGGGGGCTG CCCTGCCGACTGGGTGTGGATCGTGCCCCCCATGTCCGGAAGCATCACCCCTGTGTTCCACCAGGAGATG CTCAACTACCGGCTCACCCCCTCCTTCGAATACCAGCCTGATCCCTGGAACACGCATGTCTGGAAAGGCA CCAACGGGACCCCCACAAAGCGGCGAGCCATTGGCTTCAAGAAGCTAGCAGAAGCTGTCAAGTTCTCGGC CAAGCTGATGGGGCAGGCTATGGCCAAGAGGGTGAAAGCGACCATCCTCTATGCCACAGAGACAGGCAAA TCGCAAGCTTATGCCAAGACCTTGTGTGAGATCTTCAAACACGCCTTTGATGCCAAGGTGATGTCCATGG AAGAATATGACATTGTGCACCTGGAACATGAAACTCTGGTCCTTGTGGTCACCAGCACCTTTGGCAATGG AGATCCCCCTGAGAATGGGGAGAAATTCGGCTGTGCTTTGATGGAAATGAGGCACCCCAACTCTGTGCAG GAAGAAAGGAAGTACCCGGAACCCTTGCGTTTCTTTCCCCGTAAAGGGCCTCCCCTCCCCAATGGTGACA CAGAAGTCCACGGTCTGGCTGCAGCCCGTGACAGCCAGCACAGGAGCTACAAGGTCCGATTCAACAGCGT CTCCTCCTACTCTGACTCCCAAAAATCATCAGGCGATGGGCCCGACCTCAGAGACAACTTTGAGAGTGCT GGACCCCTGGCCAATGTGAGGTTCTCAGTTTTTGGCCTCGGCTCACGAGCATACCCTCACTTTTGCGCCT TCGGACACGCTGTGGACACCCTCCTGGAAGAACTGGGAGGGGAGAGGATCCTGAAGATGAGGGAAGGGGA TGAGCTCTGTGGGCAGGAAGAGGCTTTCAGGACCTGGGCCAAGAAGGTCTTCAAGGCAGCCTGTGATGTC TTCTGTGTGGGAGATGATGTCAACATTGAAAAGGCCAACAATTCCCTCATCAGCAATGATCGCAGCTGGA AGAGAAACAAGTTCCGCCTCACCTTTGTGGCCGAAGCTCCAGAACTCACACAAGGTCTATCCAATGTCCA CAAAAAGCGAGTCTCAGCTGCCCGGCTCCTTAGCCGTCAAAACCTCCAGAGCCCTAAATCCAGTCGGTCA ACTATCTTCGTGCGTCTCCACACCAACGGGAGCCAGGAGCTGCAGTACCAGCCTGGGGACCACCTGGGTG TCTTCCCTGGCAACCACGAGGACCTCGTGAATGCCCTGATCGAGCGGCTGGAGGACGCGCCGCCTGTCAA CCAGATGGTGAAAGTGGAACTGCTGGAGGAGCGGAACACGGCTTTAGGTGTCATCAGTAACTGGACAGAC GAGCTCCGCCTCCCGCCCTGCACCATCTTCCAGGCCTTCAAGTACTACCTGGACATCACCACGCCACCAA CGCCTCTGCAGCTGCAGCAGTTTGCCTCCCTAGCTACCAGCGAGAAGGAGAAGCAGCGTCTGCTGGTCCT CAGCAAGGGTTTGCAGGAGTACGAGGAATGGAAATGGGGCAAGAACCCCACCATCGTGGAGGTGCTGGAG GAGTTCCCATCTATCCAGATGCCGGCCACCCTGCTCCTGACCCAGCTGTCCCTGCTGCAGCCCCGCTACT ATTCCATCAGCTCCTCCCCAGACATGTACCCTGATGAAGTGCACCTCACTGTGGCCATCGTTTCCTACCG CACTCGAGATGGAGAAGGACCAATTCACCACGGCGTATGCTCCTCCTGGCTCAACCGGATACAGGCTGAC GAACTGGTCCCCTGTTTCGTGAGAGGAGCACCCAGCTTCCACCTGCCCCGGAACCCCCAAGTCCCCTGCA TCCTCGTTGGACCAGGCACCGGCATTGCCCCTTTCCGAAGCTTCTGGCAACAGCGGCAATTTGATATCCA ACACAAAGGAATGAACCCCTGCCCCATGGTCCTGGTCTTCGGGTGCCGGCAATCCAAGATAGATCATATC TACAGGGAAGAGACCCTGCAGGCCAAGAACAAGGGGGTCTTCAGAGAGCTGTACACGGCTTACTCCCGGG AGCCAGACAAACCAAAGAAGTACGTGCAGGACATCCTGCAGGAGCAGCTGGCGGAGTCTGTGTACCGAGC CCTGAAGGAGCAAGGGGGCCACATATACGTCTGTGGGGACGTCACCATGGCTGCTGATGTCCTCAAAGCC ATCCAGCGCATCATGACCCAGCAGGGGAAGCTCTCGGCAGAGGACGCCGGCGTATTCATCAGCCGGATGA GGGATGACAACCGATACCATGAGGATATTTTTGGAGTCACCCTGCGAACGTACGAAGTGACCAACCGCCT TAGATCTGAGTCCATTGCCTTCATTGAAGAGAGCAAAAAAGACACCGATGAGGTTTTCAGCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235311 representing NM_001204218
Red=Cloning site Green=Tags(s) MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGR PLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPP AGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIE PVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYS EKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRP EDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRC VGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGY KQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFK DLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLW KDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEM LNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGK SQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQ EERKYPEPLRFFPRKGPPLPNGDTEVHGLAAARDSQHRSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESA GPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDV FCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRS TIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTD ELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLE EFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQAD ELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHI YREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKA IQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001204218 |
| ORF Size | 4404 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001204218.1, NP_001191147.1 |
| RefSeq Size | 12291 bp |
| RefSeq ORF | 4407 bp |
| Locus ID | 4842 |
| UniProt ID | P29475 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer |
| Gene Summary | The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011] |
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