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RFC1 (NM_001204747) Human Tagged ORF Clone
CAT＃: RG235188
- TrueORF®
RFC1 (tGFP-tagged) - Homo sapiens replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 2
ORF Plasmid: DDK
"NM_001204747" in other vectors (2)
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CNY 12,640.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A1; CANVAS; MHCBFB; PO-GA; RECC1; RFC; RFC140 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235188 representing NM_001204747
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATTCGGAAATTCTTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAGA ATGAGAAAACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAATAG CTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATCTATGATTCAGAT TCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCAGAAAAACTGCCAGTATCTTCTA AACCTGGTAAAATTTCACGGCAGGATCCTGTTACATACATTTCAGAAACAGATGAAGAAGATGACTTTAT GTGTAAGAAGGCGGCCTCTAAATCAAAAGAGAATGGAAGATCTACAAATAGTCATCTTGGAACATCAAAC ATGAAAAAGAATGAAGAAAACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACACCCACAT CAGTACTTGATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGCAAAAGAAA AGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAATTACAGCTTGAT GAAGATGCGGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAGTTTGCCAGAACATTAGCCATGTTGGATG AAGAACCCAAGACCAAAAAGGCTCGAAAGGACACAGAAGCGGGAGAAACGTTTTCATCTGTCCAAGCCAA TTTAAGTAAAGCAGAAAAACATAAATATCCTCATAAAGTAAAAACAGCACAAGTTTCAGATGAAAGAAAG AGCTACAGTCCTAGGAAGCAAAGTAAATATGAAAGTTCAAAAGAATCTCAGCAACATTCCAAGTCATCAG CTGACAAAATAGGAGAAGTCTCTTCTCCCAAGGCCAGTTCTAAGCTGGCAATTATGAAAAGAAAAGAAGA GAGCTCTTATAAAGAAATAGAGCCTGTGGCCTCAAAAAGAAAAGAAAATGCCATTAAATTGAAAGGAGAG ACAAAAACTCCTAAGAAAACCAAAAGTTCTCCAGCTAAAAAAGAGTCTGTAAGTCCTGAAGATTCTGAAA AGAAACGCACTAATTATCAAGCTTATCGAAGCTACTTAAATCGAGAAGGTCCCAAGGCTCTGGGCTCCAA AGAAATACCAAAGGGAGCTGAAAATTGCTTGGAAGGCCTTATATTTGTAATCACAGGCGTGCTGGAGTCT ATTGAACGAGATGAGGCCAAGTCTCTAATTGAACGTTATGGGGGAAAAGTAACAGGAAATGTCAGCAAGA AAACAAATTATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGGCCGCAGCCTTGGGGAC AAAAATTATTGATGAAGATGGCCTGTTGAATCTGATTCGGACTATGCCAGGCAAGAAATCCAAGTATGAA ATAGCAGTTGAAACTGAGATGAAGAAAGAGTCCAAACTGGAGAGAACACCCCAAAAAAATGTCCAAGGAA AAAGAAAAATTAGTCCATCTAAAAAGGAATCAGAATCTAAAAAGAGCAGGCCGACTTCCAAAAGGGACAG TTTGGCAAAGACAATAAAAAAGGAAACAGATGTGTTTTGGAAAAGCCTGGATTTCAAGGAGCAGGTGGCT GAGGAGACAAGTGGTGACAGCAAGGCTAGGAATTTGGCTGATGACAGCAGTGAAAACAAAGTGGAAAATT TGCTCTGGGTGGATAAATATAAGCCAACCTCGCTCAAGACCATAATTGGACAGCAAGGTGACCAGAGCTG TGCCAACAAACTCCTACGCTGGCTCCGAAACTGGCAAAAGAGTTCTTCCGAAGATAAAAAACACGCAGCA AAGTTTGGTAAATTTTCCGGCAAAGATGATGGCTCTAGTTTTAAAGCAGCGTTGCTGTCAGGCCCTCCTG GTGTTGGCAAAACCACCACAGCTTCCCTGGTGTGTCAGGAGTTGGGATACAGCTACGTGGAACTGAATGC AAGTGACACCCGGAGTAAGAGCAGTTTGAAGGCGATTGTTGCTGAGTCACTGAACAATACCAGCATCAAA GGCTTTTATTCAAATGGAGCAGCCTCTTCAGTAAGCACGAAACATGCTCTCATCATGGATGAAGTAGATG GCATGGCAGGCAATGAGGATAGGGGAGGAATTCAGGAATTAATTGGCCTGATAAAACATACTAAAATTCC CATTATTTGTATGTGCAATGATAGAAATCATCCCAAGATTCGCTCTCTGGTTCATTATTGTTTTGATCTT CGTTTTCAAAGACCTCGGGTTGAACAGATTAAGGGTGCTATGATGTCTATTGCATTTAAAGAAGGTTTAA AGATTCCCCCTCCAGCTATGAATGAAATAATTTTGGGAGCCAATCAAGATATCAGACAGGTTTTACATAA TCTGAGTATGTGGTGTGCACGAAGTAAAGCATTAACCTATGACCAGGCCAAAGCTGATTCTCACAGAGCC AAAAAGGATATCAAAATGGGCCCATTTGATGTTGCCCGGAAAGTGTTTGCAGCTGGAGAGGAGACTGCTC ACATGTCACTTGTGGACAAGTCAGATCTCTTTTTTCATGATTATTCAATAGCACCCCTCTTCGTCCAGGA AAATTACATACACGTGAAGCCTGTAGCAGCAGGGGGTGACATGAAAAAGCACCTGATGCTTTTAAGCAGA GCAGCAGACAGCATATGCGATGGTGACCTAGTGGACAGCCAGATCCGGAGTAAGCAAAACTGGAGTCTTC TGCCTGCGCAGGCCATTTATGCCAGTGTTCTTCCTGGAGAGTTGATGAGGGGGTACATGACCCAGTTTCC CACCTTCCCAAGCTGGCTGGGGAAGCACTCGTCTACAGGCAAACATGATCGTATTGTTCAGGACCTGGCC TTGCATATGAGTCTCAGAACTTACTCCAGCAAAAGGACTGTAAACATGGATTATCTGTCGCTTCTAAGGG ATGCACTTGTACAGCCCTTGACCTCACAAGGAGTAGACGGAGTACAGGATGTTGTTGCACTTATGGACAC ATATTATTTGATGAAAGAAGACTTTGAGAATATCATGGAAATCAGCAGCTGGGGTGGCAAACCTAGTCCC TTTTCAAAGCTGGATCCCAAGGTGAAAGCAGCCTTCACAAGAGCTTACAATAAGGAAGCCCACCTTACTC CATACTCACTTCAAGCTATAAAGGCATCTAGACACAGCACAAGCCCATCCCTGGATTCGGAATACAATGA AGAATTAAATGAAGATGACTCTCAATCTGATGAGAAAGACCAAGATGCTATAGAAACTGATGCCATGATC AAGAAAAAGACAAAATCTTCAAAGCCTTCAAAACCAGAAAAAGATAAGGAGCCCAGAAAAGGAAAAGGAA AAAGTTCGAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235188 representing NM_001204747
Red=Cloning site Green=Tags(s) MDIRKFFGVIPSGKKLVSETVKKNEKTKSDEETLKAKKGIKEIKVNSSRKEDDFKQKQPSKKKRIIYDSD SESEETLQVKNAKKPPEKLPVSSKPGKISRQDPVTYISETDEEDDFMCKKAASKSKENGRSTNSHLGTSN MKKNEENTKTKNKPLSPIKLTPTSVLDYFGTGSVQRSNKKMVASKRKELSQNTDESGLNDEAIAKQLQLD EDAELERQLHEDEEFARTLAMLDEEPKTKKARKDTEAGETFSSVQANLSKAEKHKYPHKVKTAQVSDERK SYSPRKQSKYESSKESQQHSKSSADKIGEVSSPKASSKLAIMKRKEESSYKEIEPVASKRKENAIKLKGE TKTPKKTKSSPAKKESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLIFVITGVLES IERDEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKIIDEDGLLNLIRTMPGKKSKYE IAVETEMKKESKLERTPQKNVQGKRKISPSKKESESKKSRPTSKRDSLAKTIKKETDVFWKSLDFKEQVA EETSGDSKARNLADDSSENKVENLLWVDKYKPTSLKTIIGQQGDQSCANKLLRWLRNWQKSSSEDKKHAA KFGKFSGKDDGSSFKAALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKSSLKAIVAESLNNTSIK GFYSNGAASSVSTKHALIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDL RFQRPRVEQIKGAMMSIAFKEGLKIPPPAMNEIILGANQDIRQVLHNLSMWCARSKALTYDQAKADSHRA KKDIKMGPFDVARKVFAAGEETAHMSLVDKSDLFFHDYSIAPLFVQENYIHVKPVAAGGDMKKHLMLLSR AADSICDGDLVDSQIRSKQNWSLLPAQAIYASVLPGELMRGYMTQFPTFPSWLGKHSSTGKHDRIVQDLA LHMSLRTYSSKRTVNMDYLSLLRDALVQPLTSQGVDGVQDVVALMDTYYLMKEDFENIMEISSWGGKPSP FSKLDPKVKAAFTRAYNKEAHLTPYSLQAIKASRHSTSPSLDSEYNEELNEDDSQSDEKDQDAIETDAMI KKKTKSSKPSKPEKDKEPRKGKGKSSKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001204747 |
| ORF Size | 3444 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001204747.2 |
| RefSeq Size | 4899 bp |
| RefSeq ORF | 3447 bp |
| Locus ID | 5981 |
| UniProt ID | P35251 |
| Protein Families | Transcription Factors |
| Protein Pathways | DNA replication, Mismatch repair, Nucleotide excision repair |
| Gene Summary | This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011] |
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