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Phospholipase D2 (PLD2) (NM_001243108) Human Tagged ORF Clone
CAT＃: RG235039
- TrueORF®
PLD2 (tGFP-tagged) - Homo sapiens phospholipase D2 (PLD2), transcript variant 2
ORF Plasmid: DDK
"NM_001243108" in other vectors (2)
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CNY 7,890.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PLD1C |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235039 representing NM_001243108
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCGACCCCTGAGAGCCTCTTCCCCACTGGGGACGAACTGGACTCCAGCCAGCTCCAGATGGAGT CCGATGAGGTGGACACCCTGAAGGAGGGAGAGGACCCAGCCGACCGGATGCACCCGTTTCTGGCCATCTA TGAGCTTCAGTCTCTGAAAGTGCACCCCTTGGTGTTCGCACCTGGGGTCCCTGTCACAGCCCAGGTGGTG GGCACCGAAAGATATACCAGCGGATCCAAGGTGGGAACCTGCACTCTGTATTCTGTCCGCTTGACTCACG GCGACTTTTCCTGGACAACCAAGAAGAAATACCGTCATTTTCAGGAGCTGCATCGGGACCTCCTGAGACA CAAAGTCTTGATGAGTCTGCTCCCTCTGGCTCGATTTGCCGTTGCCTATTCTCCAGCCCGAGATGCAGGC AACAGAGAGATGCCCTCTCTACCCCGGGCAGGTCCTGAGGGCTCCACCAGACATGCAGCCAGCAAACAGA AATACCTGGAGAATTACCTCAACCGTCTCTTGACCATGTCTTTCTATCGCAACTACCATGCCATGACAGA GTTCCTGGAAGTCAGTCAGCTGTCCTTTATCCCGGACTTGGGCCGCAAAGGACTGGAGGGGATGATCCGG AAGCGCTCAGGTGGCCACCGTGTTCCTGGCCTCACCTGCTGTGGCCGAGACCAAGTTTGTTATCGCTGGT CCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGTGCCTCGAGACAGGTGCCATCTCATT TGTTCAGCTCTTTGACCCTGGCTTTGAGGTGCAAGTGGGGAAAAGGAGCACGGAGGCACGGCACGGCGTG CGGATCGATACCTCCCACAGGTCCTTGATTCTCAAGTGCAGCAGCTACCGGCAGGCACGGTGGTGGGCCC AAGAGATCACTGAGCTGGCACAGGGCCCAGGCAGAGACTTCCTACAGCTGCACCGGCATGACAGCTACGC CCCACCCCGGCCTGGGACCTTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTTGCTGCTGTGGCAGAT GCCATCCTTCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCTGAGGTTTACCTGAAGC GTCCGGCCCATTCAGATGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAGGAGGGTGTCCGTGT GTCTATTCTGCTGTTTAAAGAAGTGGAATTGGCCTTGGGCATCAACAGTGGCTATAGCAAGAGGGCGCTG ATGCTGCTGCACCCCAACATAAAGGTGATGCGTCACCCAGACCAAGTGACGTTGTGGGCCCATCATGAGA AGCTCCTGGTGGTGGACCAAGTGGTAGCATTCCTGGGGGGACTGGACCTTGCCTATGGCCGCTGGGATGA CCTGCACTACCGACTGACTGACCTTGGAGACTCCTCTGAATCAGCTGCCTCCCAGCCTCCCACCCCGCGC CCAGACTCACCAGCCACCCCAGACCTCTCTCACAACCAATTCTTCTGGCTGGGCAAGGACTACAGCAATC TTATCACCAAGGACTGGGTGCAGCTGGACCGGCCTTTCGAAGATTTCATTGACAGGGAGACGACCCCTCG GATGCCATGGCGGGACGTTGGGGTGGTCGTCCATGGCCTACCGGCCCGGGACCTTGCCCGGCACTTCATC CAGCGCTGGAACTTCACCAAGACCACCAAGGCCAAGTACAAGACTCCCACATACCCCTACCTGCTTCCCA AGTCTACCAGCACGGCCAATCAGCTCCCCTTCACACTTCCAGGAGGGCAGTGCACCACCGTACAGGTCTT GCGATCAGTGGACCGCTGGTCAGCAGGGACTCTGGAGAACTCCATCCTCAATGCCTACCTGCACACCATC AGGGAGAGCCAGCACTTCCTCTACATTGAGAATCAGTTCTTCATTAGCTGCTCAGATGGGCGGACGGTTC TGAACAAGGTGGGCGATGAGATTGTGGACAGAATCCTGAAGGCCCACAAACAGGGGTGGTGTTACCGAGT CTACGTGCTTTTGCCCTTACTCCCTGGCTTCGAGGGTGACATCTCCACGGGCGGTGGCAACTCCATCCAG GCCATTCTGCACTTTACTTACAGGACCCTGTGTCGTGGGGAGTATTCAATCCTGCATCGCCTTAAAGCAG CCATGGGGACAGCATGGCGGGACTATATTTCCATCTGCGGGCTTCGTACACACGGAGAGCTGGGCGGGCA CCCCGTCTCGGAGCTCATCTACATCCACAGCAAGGTGCTCATCGCAGATGACCGGACAGTCATCATTGGT TCTGCAAACATCAATGACCGGAGCTTGCTGGGGAAGCGGGACAGTGAGCTGGCCGTGCTGATCGAGGACA CAGAGACGGAACCATCCCTCATGAATGGGGCAGAGTATCAGGCGGGCAGTGTGATTCTTGGAGCAAATAC CCGGCCAGACTTGGATCTCCGAGACCCCATCTGTGATGACTTCTTCCAGTTGTGGCAAGACATGGCTGAG AGCAACGCCAATATCTATGAGCAGATCTTCCGCTGCCTGCCATCCAATGCCACGCGTTCCCTGCGGACTC TCCGGGAGTACGTGGCCGTGGAGCCCTTGGCCACGGTCAGTCCCCCCTTGGCTCGGTCTGAGCTCACCCA GGTCCAGGGCCACCTGGTCCACTTCCCCCTCAAGTTCCTAGAGGATGAGTCTTTGCTGCCCCCGCTGGGT AGCAAGGAGGGCATGATCCCCCTAGAAGTGTGGACA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235039 representing NM_001243108
Red=Cloning site Green=Tags(s) MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVV GTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAG NREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIR KRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGV RIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVAD AILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRAL MLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPR PDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFI QRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTI RESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQ AILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIG SANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGSVILGANTRPDLDLRDPICDDFFQLWQDMAE SNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLG SKEGMIPLEVWT SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001243108 |
| ORF Size | 2766 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001243108.2 |
| RefSeq Size | 3481 bp |
| RefSeq ORF | 2769 bp |
| Locus ID | 5338 |
| UniProt ID | O14939 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways |
| Gene Summary | The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] |
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