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GRIA1 (NM_001258022) Human Tagged ORF Clone
CAT＃: RG235031
- TrueORF®
GRIA1 (tGFP-tagged) - Homo sapiens glutamate receptor, ionotropic, AMPA 1 (GRIA1), transcript variant 6
ORF Plasmid: DDK
"NM_001258022" in other vectors (2)
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CNY 7,890.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GluA1; GLUH1; GLUR1; GLURA; HBGR1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235031 representing NM_001258022
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCTGCAGTACCCATCTCTTTCAGCCTCTCCAGCTAGCTGGCGGCTTGGAGTGGCCATGGAGTAACT TGCTTTGTTTCCTGACACCTGTTAAGCTACATCCTGAAGTGTGGGGATTATTTCCAAACCAGCAGTCACA GGAACATGCTGCTTTTAGATTTGCTTTGTCGCAACTCACAGAGCCCCCGAAGCTGCTCCCCCAGATTGAT ATTGTGAACATCAGCGACAGCTTTGAGATGACCTATAGATTCTGTTCCCAGTTCTCCAAAGGAGTCTATG CCATCTTTGGGTTTTATGAACGTAGGACTGTCAACATGCTGACCTCCTTTTGTGGGGCCCTCCACGTCTG CTTCATTACGCCGAGCTTTCCCGTTGATACATCCAATCAGTTTGTCCTTCAGCTGCGCCCTGAACTGCAG GATGCCCTCATCAGCATCATTGACCATTACAAGTGGCAGAAATTTGTCTACATTTATGATGCCGACCGGG GCTTATCCGTCCTGCAGAAAGTCCTGGATACAGCTGCTGAGAAGAACTGGCAGGTGACAGCAGTCAACAT TTTGACAACCACAGAGGAGGGATACCGGATGCTCTTTCAGGACCTGGAGAAGAAAAAGGAGCGGCTGGTG GTGGTGGACTGTGAATCAGAACGCCTCAATGCTATCTTGGGCCAGATTATAAAGCTAGAGAAGAATGGCA TCGGCTACCACTACATTCTTGCAAATCTGGGCTTCATGGACATTGACTTAAACAAATTCAAGGAGAGTGG CGCCAATGTGACAGGTTTCCAGCTGGTGAACTACACAGACACTATTCCGGCCAAGATCATGCAGCAGTGG AAGAATAGTGATGCTCGAGACCACACACGGGTGGACTGGAAGAGACCCAAGTACACCTCTGCGCTCACCT ACGATGGGGTGAAGGTGATGGCTGAGGCTTTCCAGAGCCTGCGGAGGCAGAGAATTGATATATCTCGCCG GGGGAATGCTGGGGATTGTCTGGCTAACCCAGCTGTTCCCTGGGGCCAAGGGATCGACATCCAGAGAGCT CTGCAGCAGGTGCGATTTGAAGGTTTAACAGGAAACGTGCAGTTTAATGAGAAAGGACGCCGGACCAACT ACACGCTCCACGTGATTGAAATGAAACATGACGGCATCCGAAAGATTGGTTACTGGAATGAAGATGATAA GTTTGTCCCTGCAGCCACCGATGCCCAAGCTGGGGGCGATAATTCAAGTGTTCAGAACAGAACATACATC GTCACAACAATCCTAGAAGATCCTTATGTGATGCTCAAGAAGAACGCCAATCAGTTTGAGGGCAATGACC GTTACGAGGGCTACTGTGTAGAGCTGGCGGCAGAGATTGCCAAGCACGTGGGCTACTCCTACCGTCTGGA GATTGTCAGTGATGGAAAATACGGAGCCCGAGACCCTGACACGAAGGCCTGGAATGGCATGGTGGGAGAG CTGGTCTATGGAAGAGCAGATGTGGCTGTGGCTCCCTTAACTATCACTTTGGTCCGGGAAGAAGTTATAG ATTTCTCCAAACCATTTATGAGTTTGGGGATCTCCATCATGATTAAAAAACCACAGAAATCCAAGCCGGG TGTCTTCTCCTTCCTTGATCCTTTGGCTTATGAGATTTGGATGTGCATTGTTTTTGCCTACATTGGAGTG AGTGTTGTCCTCTTCCTGGTCAGCCGCTTCAGTCCCTATGAATGGCACAGTGAAGAGTTTGAGGAAGGAC GGGACCAGACAACCAGTGACCAGTCCAATGAGTTTGGGATATTCAACAGTTTGTGGTTCTCCCTGGGAGC CTTCATGCAGCAAGGATGTGACATTTCTCCCAGGTCCCTGTCTGGTCGCATCGTTGGTGGCGTCTGGTGG TTCTTCACCTTAATCATCATCTCCTCATATACAGCCAATCTGGCCGCCTTCCTGACCGTGGAGAGGATGG TGTCTCCCATTGAGAGTGCAGAGGACCTAGCGAAGCAGACAGAAATTGCCTACGGGACGCTGGAAGCAGG ATCTACTAAGGAGTTCTTCAGGAGGTCTAAAATTGCTGTGTTTGAGAAGATGTGGACATACATGAAGTCA GCAGAGCCATCAGTTTTTGTGCGGACCACAGAGGAGGGGATGATTCGAGTGAGGAAATCCAAAGGCAAAT ATGCCTACCTCCTGGAGTCCACCATGAATGAGTACATTGAGCAGCGGAAACCCTGTGACACCATGAAGGT GGGAGGTAACTTGGATTCCAAAGGCTATGGCATTGCAACACCCAAGGGGTCTGCCCTGAGAGGTCCCGTA AACCTAGCGGTTTTGAAACTCAGTGAGCAAGGCGTCTTAGACAAGCTGAAAAGCAAATGGTGGTACGATA AAGGGGAATGTGGAAGCAAGGACTCCGGAAGTAAGGACAAGACAAGCGCTCTGAGCCTCAGCAATGTGGC AGGCGTGTTCTACATCCTGATCGGAGGACTTGGACTAGCCATGCTGGTTGCCTTAATCGAGTTCTGCTAC AAATCCCGTAGTGAATCCAAGCGGATGAAGGGTTTTTGTTTGATCCCACAGCAATCCATCAACGAAGCCA TACGGACATCGACCCTCCCCCGCAACAGCGGGGCAGGAGCCAGCAGCGGCGGCAGTGGAGAGAATGGTCG GGTGGTCAGCCATGACTTCCCCAAGTCCATGCAATCGATTCCTTGCATGAGCCACAGTTCAGGGATGCCC TTGGGAGCCACGGGATTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235031 representing NM_001258022
Red=Cloning site Green=Tags(s) MCCSTHLFQPLQLAGGLEWPWSNLLCFLTPVKLHPEVWGLFPNQQSQEHAAFRFALSQLTEPPKLLPQID IVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQ DALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLV VVDCESERLNAILGQIIKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQW KNSDARDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRA LQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYI VTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGE LVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGV SVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWW FFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKS AEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPV NLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCY KSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASSGGSGENGRVVSHDFPKSMQSIPCMSHSSGMP LGATGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001258022 |
| ORF Size | 2748 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001258022.1, NP_001244951.1 |
| RefSeq Size | 5439 bp |
| RefSeq ORF | 2751 bp |
| Locus ID | 2890 |
| UniProt ID | P42261 |
| Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
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