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PDE4A (NM_001243121) Human Tagged ORF Clone
CAT＃: RG234973
- TrueORF®
PDE4A (tGFP-tagged) - Homo sapiens phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 5
ORF Plasmid: DDK
"NM_001243121" in other vectors (2)
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CNY 7,890.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DPDE2; PDE4; PDE46 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234973 representing NM_001243121
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGGAGTCGCAGTGCCCTGTCCGTGGCAGGGACCGGGGACGAGAGGTCGAGGGAGACCCCCGAAT CCGACCGTGCCAACATGCTGGGGGCCGACCTGCGTCGCCCTCGCCGCCGCCTCTCGTCCGGTCCTGGCCT GGGCTGGGCCCAGCCTGAGCCCTCGGACCCTGGGGTCCCTCTGCCGCCACGGCCCACCACCCTGCCGCTG CTGATCCCACCGCGGATTTCCATCACCAGGGCCGAGAACGACAGCTTCGAGGCAGAGAATGGGCCGACAC CATCTCCTGGCCGCAGCCCCCTGGACTCGCAGGCGAGCCCAGGACTCGTGCTGCACGCCGGGGCGGCCAC CAGCCAGCGCCGGGAGTCCTTCCTGTACCGCTCAGACAGCGACTATGACATGTCACCCAAGACCATGTCC CGGAACTCATCGGTCACCAGCGAGGCGCACGCTGAAGACCTCATCGTAACACCATTTGCTCAGGTGCTGG CCAGCCTCCGGAGCGTCCGTAGCAACTTCTCACTCCTGACCAATGTGCCCGTTCCCAGTAACAAGCGGTC CCCGCTGGGCGGCCCCACCCCTGTCTGCAAGGCCACGCTGTCAGAAGAAACGTGTCAGCAGTTGGCCCGG GAGACTCTGGAGGAGCTGGACTGGTGTCTGGAGCAGCTGGAGACCATGCAGACCTATCGCTCTGTCAGCG AGATGGCCTCGCACAAGTTCAAAAGGATGTTGAACCGTGAGCTCACACACCTGTCAGAAATGAGCAGGTC CGGAAACCAGGTCTCAGAGTACATTTCCACAACATTCCTGGACAAACAGAATGAAGTGGAGATCCCATCA CCCACGATGAAGGAACGAGAAAAACAGCAAGCGCCGCGACCAAGACCCTCCCAGCCGCCCCCGCCCCCTG TACCACACTTACAGCCCATGTCCCAAATCACAGGGTTGAAAAAGTTGATGCATAGTAACAGCCTGAACAA CTCTAACATTCCCCGATTTGGGGTGAAGACCGATCAAGAAGAGCTCCTGGCCCAAGAACTGGAGAACCTG AACAAGTGGGGCCTGAACATCTTTTGCGTGTCGGATTACGCTGGAGGCCGCTCACTCACCTGCATCATGT ACATGATATTCCAGGAGCGGGACCTGCTGAAGAAATTCCGCATCCCTGTGGACACGATGGTGACATACAT GCTGACGCTGGAGGATCACTACCACGCTGACGTGGCCTACCATAACAGCCTGCACGCAGCTGACGTGCTG CAGTCCACCCACGTACTGCTGGCCACGCCTGCACTAGATGCAGTGTTCACGGACCTGGAGATTCTCGCCG CCCTCTTCGCGGCTGCCATCCACGATGTGGATCACCCTGGGGTCTCCAACCAGTTCCTCATCAACACCAA TTCGGAGCTGGCGCTCATGTACAACGATGAGTCGGTGCTCGAGAATCACCACCTGGCCGTGGGCTTCAAG CTGCTGCAGGAGGACAACTGCGACATCTTCCAGAACCTCAGCAAGCGCCAGCGGCAGAGCCTACGCAAGA TGGTCATCGACATGGTGCTGGCCACGGACATGTCCAAGCACATGACCCTCCTGGCTGACCTGAAGACCAT GGTGGAGACCAAGAAAGTGACCAGCTCAGGGGTCCTCCTGCTAGATAACTACTCCGACCGCATCCAGGTC CTCCGGAACATGGTGCACTGTGCCGACCTCAGCAACCCCACCAAGCCGCTGGAGCTGTACCGCCAGTGGA CAGACCGCATCATGGCCGAGTTCTTCCAGCAGGGTGACCGAGAGCGCGAGCGTGGCATGGAAATCAGCCC CATGTGTGACAAGCACACTGCCTCCGTGGAGAAGTCTCAGGTGGGTTTTATTGACTACATTGTGCACCCA TTGTGGGAGACCTGGGCGGACCTTGTCCACCCAGATGCCCAGGAGATCTTGGACACTTTGGAGGACAACC GGGACTGGTACTACAGCGCCATCCGGCAGAGCCCATCTCCGCCACCCGAGGAGGAGTCAAGGGGGCCAGG CCACCCACCCCTGCCTGACAAGTTCCAGTTTGAGCTGACGCTGGAGGAGGAAGAGGAGGAAGAAATATCA ATGGCCCAGATACCGTGCACAGCCCAAGAGGCATTGACTGCGCAGGGATTGTCAGGAGTCGAGGAAGCTC TGGATGCAACCATAGCCTGGGAGGCATCCCCGGCCCAGGAGTCGTTGGAAGTTATGGCACAGGAAGCATC CCTGGAGGCCGAGCTGGAGGCAGTGTATTTGACACAGCAGGCACAGTCCACAGGCAGTGCACCTGTGGCT CCGGATGAGTTCTCGTCCCGGGAGGAATTCGTGGTTGCTGTAAGCCACAGCAGCCCCTCTGCCCTGGCTC TTCAAAGCCCCCTTCTCCCTGCTTGGAGGACCCTGTCTGTTTCAGAGCATGCCCCGGGCCTCCCGGGCCT CCCCTCCACGGCGGCCGAGGTGGAGGCCCAACGAGAGCACCAGGCTGCCAAGAGGGCTTGCAGTGCCTGC GCAGGGACATTTGGGGAGGACACATCCGCACTCCCAGCTCCTGGTGGCGGGGGGTCAGGTGGAGACCCTA CC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234973 representing NM_001243121
Red=Cloning site Green=Tags(s) MKRSRSALSVAGTGDERSRETPESDRANMLGADLRRPRRRLSSGPGLGWAQPEPSDPGVPLPPRPTTLPL LIPPRISITRAENDSFEAENGPTPSPGRSPLDSQASPGLVLHAGAATSQRRESFLYRSDSDYDMSPKTMS RNSSVTSEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNVPVPSNKRSPLGGPTPVCKATLSEETCQQLAR ETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSEYISTTFLDKQNEVEIPS PTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLKKLMHSNSLNNSNIPRFGVKTDQEELLAQELENL NKWGLNIFCVSDYAGGRSLTCIMYMIFQERDLLKKFRIPVDTMVTYMLTLEDHYHADVAYHNSLHAADVL QSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFK LLQEDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQV LRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHP LWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGHPPLPDKFQFELTLEEEEEEEIS MAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSAPVA PDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQREHQAAKRACSAC AGTFGEDTSALPAPGGGGSGGDPT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001243121 |
| ORF Size | 2592 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001243121.1, NP_001230050.1 |
| RefSeq Size | 4704 bp |
| RefSeq ORF | 2595 bp |
| Locus ID | 5141 |
| UniProt ID | P27815 |
| Protein Families | Druggable Genome |
| Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
| Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
Documents
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