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R Cadherin (CDH4) (NM_001252339) Human Tagged ORF Clone
CAT＃: RG234952
- TrueORF®
CDH4 (tGFP-tagged) - Homo sapiens cadherin 4, type 1, R-cadherin (retinal) (CDH4), transcript variant 3
ORF Plasmid: DDK
"NM_001252339" in other vectors (2)
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CNY 7,890.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAD4; R-CAD; RCAD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234952 representing NM_001252339
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTCAAAGTTGGGGCAGATGGGACAGTCTTCGCCACCCGGGAGCTGCAGGTCCCCTCCGAGCAGG TGGCGTTCACGGTGACTGCATGGGACAGCCAGACAGCAGAGAAATGGGACGCCGTGGTGCGGTTGCTGGT GGCCCAGACCTCGTCCCCGCACTCTGGACACAAGCCGCAGAAAGGAAAGAAGGTCGTGGCTCTGGACCCC TCTCCGCCTCCGAAGGACACCCTGCTGCCGTGGCCCCAGCACCAGAACGCCAACGGGCTGAGGCGGCGCA AACGGGACTGGGTCATCCCGCCCATCAACGTGCCCGAGAACTCGCGCGGGCCCTTCCCGCAGCAGCTCGT GAGGATCCGGTCCGACAAAGACAATGACATCCCCATCCGGTACAGCATCACGGGAGTGGGCGCCGACCAG CCCCCCATGGAGGTCTTCAGCATTGACTCCATGTCCGGCCGGATGTACGTCACAAGGCCCATGGACCGGG AGGAGCACGCCTCTTACCACCTCCGAGCCCACGCTGTGGACATGAATGGCAACAAGGTGGAGAACCCCAT CGACCTGTACATCTACGTCATCGACATGAATGACAACCGCCCTGAGTTCATCAACCAGGTCTACAACGGC TCCGTGGACGAGGGCTCCAAGCCAGGCACCTACGTGATGACCGTCACGGCCAACGATGCTGACGACAGCA CCACGGCCAACGGGATGGTGCGGTACCGGATCGTGACCCAGACCCCACAGAGCCCGTCCCAGAATATGTT CACCATCAACAGCGAGACTGGAGATATCGTCACAGTGGCGGCTGGCCTGGACCGAGAGAAAGTTCAGCAG TACACAGTCATCGTTCAGGCCACAGATATGGAAGGAAATCTCAACTATGGCCTCTCAAACACAGCCACAG CCATCATCACGGTGACAGATGTGAATGACAACCCGCCAGAATTTACCGCCAGCACGTTTGCAGGGGAGGT CCCCGAAAACCGCGTGGAGACCGTGGTCGCAAACCTCACGGTGATGGACCGAGATCAGCCCCACTCTCCA AACTGGAATGCCGTTTACCGCATCATCAGTGGGGATCCATCCGGGCACTTCAGCGTCCGCACAGACCCCG TAACCAACGAGGGCATGGTCACCGTGGTGAAGGCAGTCGACTACGAGCTCAACAGAGCTTTCATGCTGAC AGTGATGGTGTCCAACCAGGCGCCCCTGGCCAGCGGAATCCAGATGTCCTTCCAGTCCACGGCAGGGGTG ACCATCTCCATCATGGACATCAACGAGGCTCCCTACTTCCCCTCAAACCACAAGCTGATCCGCCTGGAGG AGGGCGTGCCCCCCGGCACCGTGCTGACCACGTTTTCAGCTGTGGACCCTGACCGGTTCATGCAGCAGGC TGTGAGATACTCAAAGCTGTCAGACCCAGCGAGCTGGCTGCACATCAATGCCACCAACGGCCAGATCACC ACGGCGGCAGTGCTGGACCGTGAGTCCCTCTACACCAAAAACAACGTCTACGAGGCCACCTTCCTGGCAG CTGACAATGGGATACCCCCGGCCAGCGGCACCGGGACCCTCCAGATCTATCTCATTGACATCAACGACAA CGCCCCTGAGCTGCTGCCCAAGGAGGCGCAGATCTGCGAGAAGCCCAACCTGAACGCCATCAACATCACG GCGGCCGACGCTGACGTCGACCCCAACATCGGCCCCTACGTCTTCGAGCTGCCCTTTGTCCCGGCGGCCG TGCGGAAGAACTGGACCATCACCCGCCTGAACGGTGACTATGCCCAACTCAGCTTGCGCATCCTGTACCT GGAGGCCGGGATGTATGACGTCCCCATCATCGTCACAGACTCTGGAAACCCTCCCCTGTCCAACACGTCC ATCATCAAAGTCAAGGTGTGCCCATGTGATGACAACGGGGACTGCACCACCATTGGCGCAGTGGCAGCGG CTGGTCTGGGCACCGGTGCCATCGTGGCCATCCTCATCTGCATCCTCATCCTGCTGACCATGGTCCTGCT GTTTGTCATGTGGATGAAGCGGCGAGAGAAGGAGCGCCACACGAAGCAGCTGCTCATTGACCCCGAGGAC GACGTCCGCGACAACATCCTCAAGTATGACGAGGAAGGCGGTGGCGAGGAGGACCAGGACTACGACCTCA GCCAGCTGCAGCAGCCGGAAGCCATGGGGCACGTGCCAAGCAAAGCCCCTGGCGTGCGTCGCGTGGATGA GCGGCCGGTGGGCGCTGAGCCCCAGTACCCGATCAGGCCCATGGTGCCGCACCCAGGCGACATCGGTGAC TTCATCAATGAGGGACTCCGCGCTGCTGACAACGACCCCACGGCACCCCCCTATGACTCCCTGCTGGTCT TCGACTACGAGGGGAGCGGCTCCACCGCAGGCTCCGTCAGCTCCCTGAACTCATCCAGTTCCGGGGACCA AGACTACGATTACCTCAACGACTGGGGGCCCAGATTCAAGAAGCTGGCGGACATGTATGGAGGTGGTGAA GAGGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234952 representing NM_001252339
Red=Cloning site Green=Tags(s) MDFKVGADGTVFATRELQVPSEQVAFTVTAWDSQTAEKWDAVVRLLVAQTSSPHSGHKPQKGKKVVALDP SPPPKDTLLPWPQHQNANGLRRRKRDWVIPPINVPENSRGPFPQQLVRIRSDKDNDIPIRYSITGVGADQ PPMEVFSIDSMSGRMYVTRPMDREEHASYHLRAHAVDMNGNKVENPIDLYIYVIDMNDNRPEFINQVYNG SVDEGSKPGTYVMTVTANDADDSTTANGMVRYRIVTQTPQSPSQNMFTINSETGDIVTVAAGLDREKVQQ YTVIVQATDMEGNLNYGLSNTATAIITVTDVNDNPPEFTASTFAGEVPENRVETVVANLTVMDRDQPHSP NWNAVYRIISGDPSGHFSVRTDPVTNEGMVTVVKAVDYELNRAFMLTVMVSNQAPLASGIQMSFQSTAGV TISIMDINEAPYFPSNHKLIRLEEGVPPGTVLTTFSAVDPDRFMQQAVRYSKLSDPASWLHINATNGQIT TAAVLDRESLYTKNNVYEATFLAADNGIPPASGTGTLQIYLIDINDNAPELLPKEAQICEKPNLNAINIT AADADVDPNIGPYVFELPFVPAAVRKNWTITRLNGDYAQLSLRILYLEAGMYDVPIIVTDSGNPPLSNTS IIKVKVCPCDDNGDCTTIGAVAAAGLGTGAIVAILICILILLTMVLLFVMWMKRREKERHTKQLLIDPED DVRDNILKYDEEGGGEEDQDYDLSQLQQPEAMGHVPSKAPGVRRVDERPVGAEPQYPIRPMVPHPGDIGD FINEGLRAADNDPTAPPYDSLLVFDYEGSGSTAGSVSSLNSSSSGDQDYDYLNDWGPRFKKLADMYGGGE ED TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001252339 |
| ORF Size | 2526 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001252339.3 |
| RefSeq Size | 6500 bp |
| RefSeq ORF | 2529 bp |
| Locus ID | 1002 |
| UniProt ID | P55283 |
| Protein Families | Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| Gene Summary | This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
Documents
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