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ADAM15 (NM_001261464) Human Tagged ORF Clone
CAT＃: RG234934
- TrueORF®
ADAM15 (tGFP-tagged) - Homo sapiens ADAM metallopeptidase domain 15 (ADAM15), transcript variant 7
ORF Plasmid: DDK
"NM_001261464" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MDC15 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234934 representing NM_001261464
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTGGCGCTGCTCTGGGCCCTGGGGCTCCTGGGCGCGGGCAGCCCTCTGCCTTCCTGGCCGCTCC CAAATATAGTACTGTCTTGGGGTGTCCTGGGACCTGCAGGTGGCACTGAGGAGCAGCAGGCAGAGTCAGA GAAGGCCCCGAGGGAGCCCTTGGAGCCCCAGGTCCTTCAGGACGATCTCCCAATTAGCCTCAAAAAGGTG CTTCAGACCAGTCTGCCTGAGCCCCTGAGGATCAAGTTGGAGCTGGACGGTGACAGTCATATCCTGGAGC TGCTACAGAATAGGGAGTTGGTCCCAGGCCGCCCAACCCTGGTGTGGTACCAGCCCGATGGCACTCGGGT GGTCAGTGAGGGACACACTTTGGAGAACTGCTGCTACCAGGGAAGAGTGCGGGGATATGCAGGCTCCTGG GTGTCCATCTGCACCTGCTCTGGGCTCAGAGGCTTGGTGGTCCTGACCCCAGAGAGAAGCTATACCCTGG AGCAGGGGCCTGGGGACCTTCAGGGTCCTCCCATTATTTCGCGAATCCAAGATCTCCACCTGCCAGGCCA CACCTGTGCCCTGAGCTGGCGGGAATCTGTACACACTCAGAAGCCACCAGAGCACCCCCTGGGACAGCGC CACATTCGCCGGAGGCGGGATGTGGTAACAGAGACCAAGACTGTGGAGTTGGTGATTGTGGCTGATCACT CGGAGGCCCAGAAATACCGGGACTTCCAGCACCTGCTAAACCGCACACTGGAAGTGGCCCTCTTGCTGGA CACATTCTTCCGGCCCCTGAATGTACGAGTGGCACTAGTGGGCCTGGAGGCCTGGACCCAGCGTGACCTG GTGGAGATCAGCCCAAACCCAGCTGTCACCCTCGAAAACTTCCTCCACTGGCGCAGGGCACATTTGCTGC CTCGATTGCCCCATGACAGTGCCCAGCTGGTGACTGGTACTTCATTCTCTGGGCCTACGGTGGGCATGGC CATTCAGAACTCCATCTGTTCTCCTGACTTCTCAGGAGGTGTGAACATGGACCACTCCACCAGCATCCTG GGAGTCGCCTCCTCCATAGCCCATGAGTTGGGCCACAGCCTGGGCCTGGACCATGATTTGCCTGGGAATA GCTGCCCCTGTCCAGGTCCAGCCCCAGCCAAGACCTGCATCATGGAGGCCTCCACAGACTTCCTACCAGG CCTGAACTTCAGCAACTGCAGCCGACGGGCCCTGGAGAAAGCCCTCCTGGATGGAATGGGCAGCTGCCTC TTCGAACGGCTGCCTAGCCTACCCCCTATGGCTGCTTTCTGCGGAAATATGTTTGTGGAGCCGGGCGAGC AGTGTGACTGTGGCTTCCTGGATGACTGCGTCGATCCCTGCTGTGATTCTTTGACCTGCCAGCTGAGGCC AGGTGCACAGTGTGCATCTGACGGACCCTGTTGTCAAAATTGCCAGCTGCGCCCGTCTGGCTGGCAGTGT CGTCCTACCAGAGGGGATTGTGACTTGCCTGAATTCTGCCCAGGAGACAGCTCCCAGTGTCCCCCTGATG TCAGCCTAGGGGATGGCGAGCCCTGCGCTGGCGGGCAAGCTGTGTGCATGCACGGGCGTTGTGCCTCCTA TGCCCAGCAGTGCCAGTCACTTTGGGGACCTGGAGCCCAGCCCGCTGCGCCACTTTGCCTCCAGACAGCT AATACTCGGGGAAATGCTTTTGGGAGCTGTGGGCGCAACCCCAGTGGCAGTTATGTGTCCTGCACCCCTA GAGATGCCATTTGTGGGCAGCTCCAGTGCCAGACAGGTAGGACCCAGCCTCTGCTGGGCTCCATCCGGGA TCTACTCTGGGAGACAATAGATGTGAATGGGACTGAGCTGAACTGCAGCTGGGTGCACCTGGACCTGGGC AGTGATGTGGCCCAGCCCCTCCTGACTCTGCCTGGCACAGCCTGTGGCCCTGGCCTGGTGTGTATAGACC ATCGATGCCAGCGTGTGGATCTCCTGGGGGCACAGGAATGTCGAAGCAAATGCCATGGACATGGGGTCTG TGACAGCAACAGGCACTGCTACTGTGAGGAGGGCTGGGCACCCCCTGACTGCACCACTCAGCTCAAAGCA ACCAGCTCCCTGACCACAGGGCTGCTCCTCAGCCTCCTGGTCTTATTGGTCCTGGTGATGCTTGGTGCCA GCTACTGGTACCGTGCCCGCCTGCACCAGCGACTCTGCCAGCTCAAGGGACCCACCTGCCAGTACAGGGC AGCCCAATCTGGTCCCTCTGAACGGCCAGGACCTCCGCAGAGGGCCCTGCTGGCACGAGGCACTAAGTCT CAGGGGCCAGCCAAGCCCCCACCCCCAAGGAAGCCACTGCCTGCCGACCCCCAGGGCCGGTGCCCATCGG GTGACCTGCCCGGCCCAGGGGCTGGAATCCCGCCCCTAGTGGTACCCTCCAGACCAGCGCCACCGCCTCC GACAGTGTCCTCGCTCTACCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234934 representing NM_001261464
Red=Cloning site Green=Tags(s) MRLALLWALGLLGAGSPLPSWPLPNIVLSWGVLGPAGGTEEQQAESEKAPREPLEPQVLQDDLPISLKKV LQTSLPEPLRIKLELDGDSHILELLQNRELVPGRPTLVWYQPDGTRVVSEGHTLENCCYQGRVRGYAGSW VSICTCSGLRGLVVLTPERSYTLEQGPGDLQGPPIISRIQDLHLPGHTCALSWRESVHTQKPPEHPLGQR HIRRRRDVVTETKTVELVIVADHSEAQKYRDFQHLLNRTLEVALLLDTFFRPLNVRVALVGLEAWTQRDL VEISPNPAVTLENFLHWRRAHLLPRLPHDSAQLVTGTSFSGPTVGMAIQNSICSPDFSGGVNMDHSTSIL GVASSIAHELGHSLGLDHDLPGNSCPCPGPAPAKTCIMEASTDFLPGLNFSNCSRRALEKALLDGMGSCL FERLPSLPPMAAFCGNMFVEPGEQCDCGFLDDCVDPCCDSLTCQLRPGAQCASDGPCCQNCQLRPSGWQC RPTRGDCDLPEFCPGDSSQCPPDVSLGDGEPCAGGQAVCMHGRCASYAQQCQSLWGPGAQPAAPLCLQTA NTRGNAFGSCGRNPSGSYVSCTPRDAICGQLQCQTGRTQPLLGSIRDLLWETIDVNGTELNCSWVHLDLG SDVAQPLLTLPGTACGPGLVCIDHRCQRVDLLGAQECRSKCHGHGVCDSNRHCYCEEGWAPPDCTTQLKA TSSLTTGLLLSLLVLLVLVMLGASYWYRARLHQRLCQLKGPTCQYRAAQSGPSERPGPPQRALLARGTKS QGPAKPPPPRKPLPADPQGRCPSGDLPGPGAGIPPLVVPSRPAPPPPTVSSLYL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001261464 |
| ORF Size | 2472 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001261464.2 |
| RefSeq Size | 2882 bp |
| RefSeq ORF | 2475 bp |
| Locus ID | 8751 |
| UniProt ID | Q13444 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Gene Summary | The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
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