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ACSL6 (NM_001205248) Human Tagged ORF Clone
CAT＃: RG234735
- TrueORF®
ACSL6 (tGFP-tagged) - Homo sapiens acyl-CoA synthetase long-chain family member 6 (ACSL6), transcript variant 4
ORF Plasmid: DDK
"NM_001205248" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ACS2; FACL6; LACS2; LACS5; LACS 6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234735 representing NM_001205248
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACACAGGAGATCCTGAGGATACTGCGACTGCCTGAGCTAGGTGACTTGGGACAGTTTTTCCGCA GCCTCTCGGCCACCACCCTCGTGAGTATGGGTGCCCTGGCTGCCATCCTTGCCTACTGGTTCACTCACCG GCCAAAGGCCTTGCAGCCGCCATGCAACCTCCTGATGCAGTCAGAAGAAGTAGAGGACAGTGGCGGGGCA CGGCGATCTGTGATTGGGTCTGGCCCTCAGCTACTTACCCACTACTATGATGATGCCCGGACCATGTACC AGGTGTTCCGCCGTGGGCTTAGCATCTCAGGGAATGGGCCCTGTCTTGGTTTCAGGAAGCCTAAGCAGCC TTACCAGTGGCTGTCCTACCAGGAGGTGGCCGACAGGGCTGAATTTCTGGGGTCCGGACTTCTCCAGCAC AATTGTAAAGCATGCACTGATCAGTTTATTGGTGTTTTTGCACAAAATCGGCCAGAGTGGATCATTGTGG AGCTGGCCTGCTACACATATTCCATGGTGGTGGTCCCGCTCTATGACACCCTGGGCCCTGGGGCTATCCG CTACATCATCAATACAGCGGACATCAGCACCGTGATTGTGGACAAACCTCAGAAGGCTGTGCTTCTGCTA GAGCATGTGGAGAGGAAGGAGACTCCAGGCCTCAAGCTGATCATCCTCATGGACCCATTCGAAGAAGCCC TGAAAGAGAGAGGGCAGAAGTGCGGGGTGGTCATTAAGTCCATGCAGGCCGTGGAGGACTGTGGCCAAGA GAATCACCAGGCTCCTGTGCCCCCGCAGCCTGATGACCTCTCCATTGTGTGTTTCACAAGCGGCACGACA GGGAACCCAAAAGGTGCGATGCTCACCCATGGGAACGTGGTGGCTGATTTCTCAGGCTTTCTGAAAGTGA CAGAGAGTCAGTGGGCTCCCACTTGTGCGGATGTGCACATTTCCTATTTGCCTTTAGCACACATGTTTGA GCGAATGGTGCAGTCTGTCGTCTATTGCCACGGAGGGCGTGTTGGCTTCTTCCAGGGAGATATCCGCCTT CTCTCAGATGACATGAAGGCTCTATGCCCCACCATCTTCCCTGTGGTCCCACGACTGCTGAACCGGATGT ACGACAAGATCTTCAGCCAGGCAAACACACCATTAAAGCGCTGGCTCCTGGAGTTTGCAGCAAAGCGTAA GCAAGCCGAGGTCCGGAGTGGAATCATCAGGAATGATAGTATCTGGGATGAACTCTTCTTTAATAAGATT CAGGCCAGTCTTGGTGGGTGTGTGCGGATGATTGTTACTGGAGCAGCCCCAGCATCACCAACAGTTCTGG GATTTCTCCGGGCAGCTCTAGGGTGCCAGGTTTATGAAGGTTATGGCCAAACTGAGTGCACAGCTGGATG TACCTTCACCACTCCTGGCGACTGGACCTCAGGGCACGTAGGGGCGCCACTTCCCTGCAATCATATCAAG CTCGTTGATGTTGAGGAACTGAACTACTGGGCCTGCAAAGGAGAGGGAGAGATATGTGTGAGAGGACCAA ATGTGTTCAAAGGCTACTTGAAAGATCCAGACAGGACGAAGGAGGCCCTGGACAGCGATGGCTGGCTTCA CACTGGAGACATCGGAAAATGGCTGCCGGCAGGAACTCTTAAAATTATTGATCGGAAAAAGCATATATTT AAACTTGCTCAGGGAGAATATGTTGCACCCGAGAAGATTGAGAACATCTACATCCGGAGCCAACCTGTGG CGCAAATCTATGTCCATGGGGACAGCTTAAAGGCCTTTTTGGTAGGCATTGTTGTGCCTGACCCTGAAGT TATGCCCTCCTGGGCCCAGAAGAGAGGAATTGAAGGAACATATGCAGATCTCTGCACAAATAAGGATCTG AAGAAAGCCATTTTGGAAGATATGGTGAGGTTAGGAAAAGAAAGTGGACTCCATTCTTTTGAGCAGGTTA AAGCCATTCACATCCATTCTGACATGTTCTCAGTTCAAAATGGCTTGCTGACACCAACACTAAAAGCTAA GAGACCTGAGCTGAGAGAGTACTTCAAAAAACAAATAGAAGAGCTTTACTCAATCTCCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234735 representing NM_001205248
Red=Cloning site Green=Tags(s) MQTQEILRILRLPELGDLGQFFRSLSATTLVSMGALAAILAYWFTHRPKALQPPCNLLMQSEEVEDSGGA RRSVIGSGPQLLTHYYDDARTMYQVFRRGLSISGNGPCLGFRKPKQPYQWLSYQEVADRAEFLGSGLLQH NCKACTDQFIGVFAQNRPEWIIVELACYTYSMVVVPLYDTLGPGAIRYIINTADISTVIVDKPQKAVLLL EHVERKETPGLKLIILMDPFEEALKERGQKCGVVIKSMQAVEDCGQENHQAPVPPQPDDLSIVCFTSGTT GNPKGAMLTHGNVVADFSGFLKVTESQWAPTCADVHISYLPLAHMFERMVQSVVYCHGGRVGFFQGDIRL LSDDMKALCPTIFPVVPRLLNRMYDKIFSQANTPLKRWLLEFAAKRKQAEVRSGIIRNDSIWDELFFNKI QASLGGCVRMIVTGAAPASPTVLGFLRAALGCQVYEGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIK LVDVEELNYWACKGEGEICVRGPNVFKGYLKDPDRTKEALDSDGWLHTGDIGKWLPAGTLKIIDRKKHIF KLAQGEYVAPEKIENIYIRSQPVAQIYVHGDSLKAFLVGIVVPDPEVMPSWAQKRGIEGTYADLCTNKDL KKAILEDMVRLGKESGLHSFEQVKAIHIHSDMFSVQNGLLTPTLKAKRPELREYFKKQIEELYSISM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001205248 |
| ORF Size | 2091 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001205248.2 |
| RefSeq Size | 6541 bp |
| RefSeq ORF | 2094 bp |
| Locus ID | 23305 |
| UniProt ID | Q9UKU0 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway |
| Gene Summary | The protein encoded by this gene catalyzes the formation of acyl-CoA from fatty acids, ATP, and CoA, using magnesium as a cofactor. The encoded protein plays a major role in fatty acid metabolism in the brain. Translocations with the ETV6 gene are causes of myelodysplastic syndrome with basophilia, acute myelogenous leukemia with eosinophilia, and acute eosinophilic leukemia. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2011] |
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