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GPR161 (NM_001267609) Human Tagged ORF Clone
CAT＃: RG234416
- TrueORF®
GPR161 (tGFP-tagged) - Homo sapiens G protein-coupled receptor 161 (GPR161), transcript variant 1
ORF Plasmid: DDK
"NM_001267609" in other vectors (2)
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CNY 8,136.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RE2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234416 representing NM_001267609
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCAAGAGGAGTCGTCCAGCATGCTCTGCCCACCCCACGCCGAGGTGCACTGACCATGAGCCTCA ACTCCTCCCTCAGCTGCAGGAAGGAGCTGAGTAATCTCACTGAGGAGGAGGGTGGCGAAGGGGGCGTCAT CATCACCCAGTTCATCGCCATCATTGTCATCACCATTTTTGTCTGCCTGGGAAACCTGGTCATCGTGGTC ACCTTGTACAAGAAGTCCTACCTCCTCACCCTCAGCAACAAGTTCGTCTTCAGCCTGACTCTGTCCAACT TCCTGCTGTCCGTGTTGGTGCTGCCTTTTGTGGTGACGAGCTCCATCCGCAGGGAATGGATCTTTGGTGT AGTGTGGTGCAACTTCTCTGCCCTCCTCTACCTGCTGATCAGCTCTGCCAGCATGCTAACCCTCGGGGTC ATTGCCATCGACCGCTACTATGCTGTCCTGTACCCCATGGTGTACCCCATGAAGATCACAGGGAACCGGG CTGTGATGGCACTTGTCTACATCTGGCTTCACTCGCTCATCGGCTGCCTGCCACCCCTGTTTGGTTGGTC ATCCGTGGAGTTTGACGAGTTCAAATGGATGTGTGTGGCTGCTTGGCACCGGGAGCCTGGCTACACGGCC TTCTGGCAGATCTGGTGTGCCCTCTTCCCCTTTCTGGTCATGCTGGTGTGCTATGGCTTCATCTTCCGCG TGGCCAGGGTCAAGGCACGCAAGGTGCACTGTGGCACAGTCGTCATCGTGGAGGAGGATGCTCAGAGGAC CGGGAGGAAGAACTCCAGCACCTCCACCTCCTCTTCAGGCAGCAGGAGGAATGCCTTTCAGGGTGTGGTC TACTCGGCCAACCAGTGCAAAGCCCTCATCACCATCCTGGTGGTCCTCGGTGCCTTCATGGTCACCTGGG GCCCCTACATGGTTGTCATCGCCTCTGAGGCCCTCTGGGGGAAAAGCTCCGTCTCCCCGAGCCTGGAGAC TTGGGCCACATGGCTGTCCTTTGCCAGCGCTGTCTGCCACCCCCTGATCTATGGACTCTGGAACAAGACA GTTCGCAAAGAACTACTGGGCATGTGCTTTGGGGACCGGTATTATCGGGAACCATTTGTGCAACGACAGA GGACTTCCAGGCTCTTCAGCATTTCCAACAGGATCACAGACCTGGGCCTGTCCCCACACCTCACTGCGCT CATGGCAGGTGGACAGCCCCTGGGGCACAGCAGCAGCACGGGGGACACTGGCTTCAGCTGCTCCCAGGAC TCAGGGACAGATATGATGCTGCTTGAGGACTACACGTCTGATGACAACCCTCCCTCTCACTGCACTTGCC CACCCAAGAGAAGGAGCTCGGTGACATTTGAGGATGAAGTGGAACAAATCAAAGAAGCTGCCAAGAACTC GATTCTTCATGTGAAAGCTGAAGTACACAAGTCCTTGGACAGTTACGCAGCAAGCTTGGCCAAAGCCATT GAGGCCGAAGCCAAAATCAACTTATTTGGGGAGGAGGCTTTGCCAGGGGTCTTGGTTACAGCACGGACTG TCCCGGGGGGCGGCTTCGGGGGCCGCCGAGGCAGCAGAACTCTTGTGAGCCAGAGGCTGCAGTTGCAGAG CATCGAAGAAGGAGATGTTTTAGCTGCCGAGCAGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234416 representing NM_001267609
Red=Cloning site Green=Tags(s) MSARGVVQHALPTPRRGALTMSLNSSLSCRKELSNLTEEEGGEGGVIITQFIAIIVITIFVCLGNLVIVV TLYKKSYLLTLSNKFVFSLTLSNFLLSVLVLPFVVTSSIRREWIFGVVWCNFSALLYLLISSASMLTLGV IAIDRYYAVLYPMVYPMKITGNRAVMALVYIWLHSLIGCLPPLFGWSSVEFDEFKWMCVAAWHREPGYTA FWQIWCALFPFLVMLVCYGFIFRVARVKARKVHCGTVVIVEEDAQRTGRKNSSTSTSSSGSRRNAFQGVV YSANQCKALITILVVLGAFMVTWGPYMVVIASEALWGKSSVSPSLETWATWLSFASAVCHPLIYGLWNKT VRKELLGMCFGDRYYREPFVQRQRTSRLFSISNRITDLGLSPHLTALMAGGQPLGHSSSTGDTGFSCSQD SGTDMMLLEDYTSDDNPPSHCTCPPKRRSSVTFEDEVEQIKEAAKNSILHVKAEVHKSLDSYAASLAKAI EAEAKINLFGEEALPGVLVTARTVPGGGFGGRRGSRTLVSQRLQLQSIEEGDVLAAEQR TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001267609 |
| ORF Size | 1647 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001267609.1, NP_001254538.1 |
| RefSeq Size | 8265 bp |
| RefSeq ORF | 1650 bp |
| Locus ID | 23432 |
| UniProt ID | Q8N6U8 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. [provided by RefSeq, Mar 2017] |
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| FAQs |
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