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PRPF4 (NM_001244926) Human Tagged ORF Clone
CAT＃: RG234345
- TrueORF®
PRPF4 (tGFP-tagged) - Homo sapiens pre-mRNA processing factor 4 (PRPF4), transcript variant 2
ORF Plasmid: DDK
"NM_001244926" in other vectors (2)
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CNY 5,700.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234345 representing NM_001244926
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCTCGCGAGCCTCTTCCACGGCAACCAAAACTAAAGCACCCGACGACTTAGTTGCTCCGGTCG TGAAGAAACCACACATCTATTATGGAAGTTTGGAAGAGAAGGAGAGGGAGCGTCTGGCCAAAGGAGAGTC TGGGATTTTGGGGAAAGACGGACTTAAAGCAGGGATCGAAGCTGGAAATATTAATATAACCTCTGGAGAA GTGTTTGAAATTGAAGAGCATATCAGCGAGCGACAGGCAGAAGTATTGGCTGAGTTTGAGAGAAGGAAGC GAGCCCGGCAGATCAATGTTTCCACAGATGACTCAGAGGTCAAAGCTTGCCTTAGAGCCTTGGGGGAACC CATCACACTTTTTGGAGAGGGTCCTGCTGAAAGAAGAGAAAGGTTAAGAAATATCCTCTCAGTTGTCGGT ACTGATGCCTTGAAAAAGACCAAAAAGGATGATGAGAAGTCTAAAAAGTCCAAAGAAGAGTATCAGCAAA CCTGGTATCATGAAGGACCAAATAGCTTGAAGGTGGCAAGACTATGGATTGCTAATTATTCGTTGCCCAG GGCAATGAAACGCTTGGAAGAGGCCCGACTCCATAAGGAGATTCCTGAGACAACAAGGACCTCCCAGATG CAAGAGCTGCACAAGTCTCTCCGGTCTTTGAATAATTTTTGCAGTCAGATTGGGGATGATCGGCCTATCT CCTACTGTCACTTTAGTCCCAATTCCAAGATGCTGGCCACAGCTTGTTGGAGTGGGCTTTGCAAGCTCTG GTCTGTTCCTGATTGCAACCTCCTTCACACTCTTCGAGGGCATAACACAAATGTAGGAGCAATTGTATTC CATCCCAAATCCACTGTCTCCTTGGACCCAAAAGATGTCAACCTGGCCTCTTGTGCGGCTGATGGCTCTG TGAAGCTTTGGAGTCTCGACAGTGATGAACCAGTGGCAGATATTGAAGGCCATACAGTGCGTGTGGCGCG GGTAATGTGGCATCCTTCAGGACGTTTCCTGGGCACCACCTGCTATGACCGTTCATGGCGCTTATGGGAT TTGGAGGCTCAAGAGGAGATCCTGCATCAGGAAGGCCATAGCATGGGTGTGTATGACATTGCCTTCCATC AAGATGGCTCTTTGGCTGGCACTGGGGGACTGGATGCATTTGGTCGAGTTTGGGACCTACGCACAGGACG TTGTATCATGTTCTTAGAAGGCCACCTGAAAGAAATCTATGGAATAAATTTCTCCCCCAATGGCTATCAC ATTGCAACCGGCAGTGGTGACAACACCTGCAAAGTGTGGGACCTCCGACAGCGGCGTTGCGTCTACACCA TCCCTGCTCATCAGAACTTAGTGACTGGTGTCAAGTTTGAGCCTATCCATGGGAACTTCTTGCTTACTGG TGCCTATGATAACACAGCCAAGATCTGGACGCACCCAGGCTGGTCCCCGCTGAAGACTCTGGCTGGCCAC GAAGGCAAAGTGATGGGCCTAGATATTTCTTCCGATGGGCAGCTCATAGCCACTTGCTCATATGACAGGA CCTTCAAGCTGTGGATGGCTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234345 representing NM_001244926
Red=Cloning site Green=Tags(s) MASSRASSTATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKDGLKAGIEAGNINITSGE VFEIEEHISERQAEVLAEFERRKRARQINVSTDDSEVKACLRALGEPITLFGEGPAERRERLRNILSVVG TDALKKTKKDDEKSKKSKEEYQQTWYHEGPNSLKVARLWIANYSLPRAMKRLEEARLHKEIPETTRTSQM QELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVF HPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWD LEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYH IATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGH EGKVMGLDISSDGQLIATCSYDRTFKLWMAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001244926 |
| ORF Size | 1563 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001244926.2 |
| RefSeq Size | 2938 bp |
| RefSeq ORF | 1566 bp |
| Locus ID | 9128 |
| UniProt ID | O43172 |
| Protein Pathways | Spliceosome |
| Gene Summary | The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016] |
Documents
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| FAQs |
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