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CYP4F3 (NM_001199209) Human Tagged ORF Clone
CAT＃: RG234340
- TrueORF®
CYP4F3 (tGFP-tagged) - Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 3 (CYP4F3), transcript variant 3
ORF Plasmid: DDK
"NM_001199209" in other vectors (2)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CPF3; CYP4F; CYPIVF3; LTB4H |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234340 representing NM_001199209
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACAGCTGAGCCTGTCCTCGCTGGGCCTTTGGCCAATGGCAGCATCCCCGTGGCTGCTCCTGCTGC TGGTTGGGGCCTCCTGGCTCCTGGCCCGCATCCTGGCCTGGACCTATACCTTCTATGACAACTGCTGCCG CCTCCGGTGTTTCCCGCAACCCCCGAAACGGAATTGGTTCTTGGGTCACCTGGGCCTGGTCACCCCCACG GAGCAGGGCATGAGGGTCCTGACTCAGCTGGTGGCCACCTACCCCCAGGGCTTTAAGGTCTGGATGGGCC CCATCTTCCCCGTCATCCGTTTTTGCCACCCCAACATCATCCGGTCTGTCATCAACGCCTCAGCTGCCAT TGTACCAAAGGACAAGGTCTTCTACAGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGCT GGTGAAAAGTGGAGCCGCCACCGTCGGATGCTGACGCCTGCCTTCCATTTCAACATCCTGAAGCCCTATA TGAAGATTTTCAATGAGAGTGTGAACATCATGCATGCCAAGTGGCAGCTCCTGGCCTCAGAGGGTAGTGC CCGTCTGGACATGTTTGAGCACATCAGCCTCATGACCTTGGACAGTCTGCAGAAATGTGTCTTCAGCTTT GACAGCCATTGCCAGGAGAAGCCCAGTGAATATATTGCCGCCATCTTGGAGCTCAGTGCCCTTGTGACAA AAAGACACCAGCAGATCCTCCTGTACATAGACTTCCTGTATTATCTCACCCCTGATGGGCAGCGTTTCCG CAGGGCCTGCCGCCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCCGCACCCTCCCTAGC CAGGGTGTTGATGACTTCCTCCAAGCCAAGGCCAAATCCAAGACTTTGGACTTCATTGATGTACTCCTGC TGAGCAAGGATGAAGATGGGAAGAAGTTGTCCGATGAGGACATAAGAGCAGAAGCTGACACCTTTATGTT TGAGGGCCATGACACCACAGCCAGTGGTCTCTCCTGGGTCCTGTACCACCTTGCAAAGCACCCGGAATAC CAGGAGCGCTGTCGGCAGGAGGTGCAAGAGCTTCTGAAGGACCGTGAGCCTAAAGAGATTGAATGGGACG ACCTGGCCCAGCTGCCCTTCCTGACCATGTGCATTAAGGAGAGCCTGAGGCTGCATCCCCCAGTCCCTGC CGTCTCTCGCTGCTGCACCCAAGACATTGTGCTCCCAGACGGCCGGGTCATCCCCAAAGGCATTATCTGC CTCATCAGTGTTTTTGGAACCCATCACAACCCAGCCGTGTGGCCGGACCCTGAGGTCTATGACCCCTTTC GCTTTGACCCAAAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCCTTCTCAGCAGGGCCCAGGAA CTGCATCGGGCAGGCGTTCGCGATGGCGGAGATGAAGGTGGTCCTGGGGCTCACGCTGCTGCGCTTCCGC GTCCTGCCTGACCACACCGAGCCCCGCAGGAAGCCGGAGCTGGTCCTGCGCGCAGAGGGCGGACTTTGGC TGCGGGTGGAGCCCCTGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234340 representing NM_001199209
Red=Cloning site Green=Tags(s) MPQLSLSSLGLWPMAASPWLLLLLVGASWLLARILAWTYTFYDNCCRLRCFPQPPKRNWFLGHLGLVTPT EQGMRVLTQLVATYPQGFKVWMGPIFPVIRFCHPNIIRSVINASAAIVPKDKVFYSFLKPWLGDGLLLSA GEKWSRHRRMLTPAFHFNILKPYMKIFNESVNIMHAKWQLLASEGSARLDMFEHISLMTLDSLQKCVFSF DSHCQEKPSEYIAAILELSALVTKRHQQILLYIDFLYYLTPDGQRFRRACRLVHDFTDAVIQERRRTLPS QGVDDFLQAKAKSKTLDFIDVLLLSKDEDGKKLSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEY QERCRQEVQELLKDREPKEIEWDDLAQLPFLTMCIKESLRLHPPVPAVSRCCTQDIVLPDGRVIPKGIIC LISVFGTHHNPAVWPDPEVYDPFRFDPKNIKERSPLAFIPFSAGPRNCIGQAFAMAEMKVVLGLTLLRFR VLPDHTEPRRKPELVLRAEGGLWLRVEPLS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199209 |
| ORF Size | 1560 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001199209.2 |
| RefSeq Size | 2972 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 4051 |
| UniProt ID | Q08477 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Arachidonic acid metabolism, Metabolic pathways |
| Gene Summary | This gene, CYP4F3, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F8, is approximately 18 kb away. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2019] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC234340 | CYP4F3 (Myc-DDK tagged) - Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 3 (CYP4F3), transcript variant 3 |
CNY 4,470.00 |
|
| SC331267 | CYP4F3 (untagged) - Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 3 (CYP4F3), transcript variant 3 |
CNY 5,040.00 |
