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PKMYT1 (NM_001258450) Human Tagged ORF Clone
CAT＃: RG234078
- TrueORF®
PKMYT1 (tGFP-tagged) - Homo sapiens protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 3
ORF Plasmid: DDK
"NM_001258450" in other vectors (2)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MYT1; PPP1R126 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234078 representing NM_001258450
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGAACGCTGGCACCAGCTGCAGCCCCGGCGGGTGTCATTCCGGGGCGAGGCCTCAGAGACTCTGC AGAGCCCTGGGTATGACCCAAGCCGGCCAGAGTCCTTCTTCCAGCAGAGCTTCCAGAGGCTCAGCCGCCT GGGCCATGGCTCCTACGGAGAGGTCTTCAAGGTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAAAG CGTTCCATGTCACCATTCCGGGGCCCCAAGGACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGCCACGAGA AGGTGGGGCAGCACCCATGCTGCGTGCGGCTGGAGCAGGCCTGGGAGGAGGGCGGCATCCTGTACCTGCA GACGGAGCTGTGCGGGCCCAGCCTGCAGCAACACTGTGAGGCCTGGGGTGCCAGCCTGCCTGAGGCCCAG GTCTGGGGCTACCTGCGGGACACGCTGCTTGCCCTGGCCCATCTGCACAGCCAGGGCCTGGTGCACCTTG ATGTCAAGCCTGCCAACATCTTCCTGGGGCCCCGGGGCCGCTGCAAGCTGGGTGACTTCGGACTGCTGGT GGAGCTGGGTACAGCAGGAGCTGGTGAGGTCCAGGAGGGAGACCCCCGCTACATGGCCCCCGAGCTGCTG CAGGGCTCCTATGGGACAGCAGCGGATGTGTTCAGTCTGGGCCTCACCATCCTGGAAGTGGCATGCAACA TGGAGCTGCCCCACGGTGGGGAGGGCTGGCAGCAGCTGCGCCAGGGCTACCTGCCCCCTGAGTTCACTGC CGGTCTGTCTTCCGAGCTGCGTTCTGTCCTTGTCATGATGCTGGAGCCAGACCCCAAGCTGCGGGCCACG GCCGAGGCCCTGCTGGCACTGCCTGTGTTGAGGCAGCCGCGGGCCTGGGGTGTGCTGTGGTGCATGGCAG CGGAGGCCCTGAGCCGAGGGTGGGCCCTGTGGCAGGCCCTGCTTGCCCTGCTCTGCTGGCTCTGGCATGG GCTGGCTCACCCTGCCAGCTGGCTACAGCCCCTGGGCCCGCCAGCCACCCCGCCTGGCTCACCACCCTGC AGTTTGCTCCTGGACAGCAGCCTCTCCAGCAACTGGGATGACGACAGCCTAGGGCCTTCACTCTCCCCTG AGGCTGTCCTGGCCCGGACTGTGGGGAGCACCTCCACCCCCCGGAGCAGGTGCACACCCAGGGATGCCCT GGACCTAAGTGACATCAACTCAGAGCCTCCTCGGGGCTCCTTCCCCTCCTTTGAGCCTCGGAACCTCCTC AGCCTGTTTGAGGACACCCTAGACCCAACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234078 representing NM_001258450
Red=Cloning site Green=Tags(s) MLERWHQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVK RSMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQ VWGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELL QGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRAT AEALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPC SLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLL SLFEDTLDPT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001258450 |
| ORF Size | 1290 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001258450.2 |
| RefSeq Size | 1980 bp |
| RefSeq ORF | 1293 bp |
| Locus ID | 9088 |
| UniProt ID | Q99640 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC234078 | PKMYT1 (Myc-DDK tagged) - Homo sapiens protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 3 |
CNY 3,990.00 |
|
| SC332717 | PKMYT1 (untagged) - Homo sapiens protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 3 |
CNY 4,090.00 |
