Nociceptin receptor (OPRL1) (NM_001200019) Human Tagged ORF Clone
CAT#: RG233898
- TrueORF®
OPRL1 (tGFP-tagged) - Homo sapiens opiate receptor-like 1 (OPRL1), transcript variant 3
ORF Plasmid: DDK
"NM_001200019" in other vectors (2)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | KOR-3; KOR3; NOCIR; NOP; NOPr; OOR; OPRL; ORL1; PNOCR |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG233898 representing NM_001200019
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCTCTTCCCCGCGCCGTTCTGGGAGGTTATCTACGGCAGCCACCTTCAGGGCAACCTGTCCC TCCTGAGCCCCAACCACAGTCTGCTGCCCCCGCATCTGCTGCTCAATGCCAGCCACGGCGCCTTCCTGCC CCTCGGGCTCAAGGTCACCATCGTGGGGCTCTACCTGGCCGTGTGTGTCGGAGGGCTCCTGGGGAACTGC CTTGTCATGTACGTCATCCTCAGGCACACCAAAATGAAGACAGCCACCAATATTTACATCTTTAACCTGG CCCTGGCCGACACTCTGGTCCTGCTGACGCTGCCCTTCCAGGGCACGGACATCCTCCTGGGCTTCTGGCC GTTTGGGAATGCGCTGTGCAAGACAGTCATTGCCATTGACTACTACAACATGTTCACCAGCACCTTCACC CTAACTGCCATGAGTGTGGATCGCTATGTAGCCATCTGCCACCCCATCCGTGCCCTCGACGTCCGCACGT CCAGCAAAGCCCAGGCTGTCAATGTGGCCATCTGGGCCCTGGCCTCTGTTGTCGGTGTTCCCGTTGCCAT CATGGGCTCGGCACAGGTCGAGGATGAAGAGATCGAGTGCCTGGTGGAGATCCCTACCCCTCAGGATTAC TGGGGCCCGGTGTTTGCCATCTGCATCTTCCTCTTCTCCTTCATCGTCCCCGTGCTCGTCATCTCTGTCT GCTACAGCCTCATGATCCGGCGGCTCCGTGGAGTCCGCCTGCTCTCGGGCTCCCGAGAGAAGGACCGGAA CCTGCGGCGCATCACTCGGCTGGTGCTGGTGGTAGTGGCTGTGTTCGTGGGCTGCTGGACGCCTGTCCAG GTCTTCGTGCTGGCCCAAGGGCTGGGGGTTCAGCCGAGCAGCGAGACTGCCGTGGCCATTCTGCGCTTCT GCACGGCCCTGGGCTACGTCAACAGCTGCCTCAACCCCATCCTCTACGCCTTCCTGGATGAGAACTTCAA GGCCTGCTTCCGCAAGTTCTGCTGTGCATCTGCCCTGCGCCGGGACGTGCAGGTGTCTGACCGCGTGCGC AGCATTGCCAAGGACGTGGCCCTGGCCTGCAAGACCTCTGAGACGGTACCGCGGCCCGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233898 representing NM_001200019
Red=Cloning site Green=Tags(s) MEPLFPAPFWEVIYGSHLQGNLSLLSPNHSLLPPHLLLNASHGAFLPLGLKVTIVGLYLAVCVGGLLGNC LVMYVILRHTKMKTATNIYIFNLALADTLVLLTLPFQGTDILLGFWPFGNALCKTVIAIDYYNMFTSTFT LTAMSVDRYVAICHPIRALDVRTSSKAQAVNVAIWALASVVGVPVAIMGSAQVEDEEIECLVEIPTPQDY WGPVFAICIFLFSFIVPVLVISVCYSLMIRRLRGVRLLSGSREKDRNLRRITRLVLVVVAVFVGCWTPVQ VFVLAQGLGVQPSSETAVAILRFCTALGYVNSCLNPILYAFLDENFKACFRKFCCASALRRDVQVSDRVR SIAKDVALACKTSETVPRPA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001200019 |
ORF Size | 1110 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001200019.2 |
RefSeq Size | 3384 bp |
RefSeq ORF | 1113 bp |
Locus ID | 4987 |
UniProt ID | P41146 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
Gene Summary | The protein encoded by this gene is a member of the 7 transmembrane-spanning G protein-coupled receptor family, and functions as a receptor for the endogenous, opioid-related neuropeptide, nociceptin/orphanin FQ. This receptor-ligand system modulates a variety of biological functions and neurobehavior, including stress responses and anxiety behavior, learning and memory, locomotor activity, and inflammatory and immune responses. A promoter region between this gene and the 5'-adjacent RGS19 (regulator of G-protein signaling 19) gene on the opposite strand functions bi-directionally as a core-promoter for both genes, suggesting co-operative transcriptional regulation of these two functionally related genes. Alternatively spliced transcript variants have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017] |
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