United States
Germany
Japan
United Kingdom
China
GPR1 (NM_001261453) Human Tagged ORF Clone
CAT＃: RG233854
- TrueORF®
GPR1 (tGFP-tagged) - Homo sapiens G protein-coupled receptor 1 (GPR1), transcript variant 4
ORF Plasmid: DDK
"NM_001261453" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233854 representing NM_001261453
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGATTTGGAGGAAACATTATTTGAAGAATTTGAAAACTATTCCTATGACCTAGACTATTACTCTC TGGAGTCTGATTTGGAGGAGAAAGTCCAGCTGGGAGTTGTTCACTGGGTCTCCCTGGTGTTATATTGTTT GGCTTTTGTTCTGGGAATTCCAGGAAATGCCATCGTCATTTGGTTCACGGGGTTCAAGTGGAAGAAGACA GTCACCACTCTGTGGTTCCTCAATCTAGCCATTGCGGATTTCATTTTTCTTCTCTTTCTGCCCCTGTACA TCTCCTATGTGGCCATGAATTTCCACTGGCCCTTTGGCATCTGGCTGTGCAAAGCCAATTCCTTCACTGC CCAGTTGAACATGTTTGCCAGTGTTTTTTTCCTGACAGTGATCAGCCTGGACCACTATATCCACTTGATC CATCCTGTCTTATCTCATCGGCATCGAACCCTCAAGAACTCTCTGATTGTCATTATATTCATCTGGCTTT TGGCTTCTCTAATTGGCGGTCCTGCCCTGTACTTCCGGGACACTGTGGAGTTCAATAATCATACTCTTTG CTATAACAATTTTCAGAAGCATGATCCTGACCTCACTTTGATCAGGCACCATGTTCTGACTTGGGTGAAA TTTATCATTGGCTATCTCTTCCCTTTGCTAACAATGAGTATTTGCTACTTGTGTCTCATCTTCAAGGTGA AGAAGCGAAGCATCCTGATCTCCAGTAGGCATTTCTGGACAATTCTGGTTGTGGTTGTGGCCTTTGTGGT TTGCTGGACTCCTTATCACCTGTTTAGCATTTGGGAGCTCACCATTCACCACAATAGCTATTCCCACCAT GTGATGCAGGCTGGAATCCCCCTCTCCACTGGTTTGGCATTCCTCAATAGTTGCTTGAACCCCATCCTTT ATGTCCTAATTAGTAAGAAGTTCCAAGCTCGCTTCCGGTCCTCAGTTGCTGAGATACTCAAGTACACACT GTGGGAAGTCAGCTGTTCTGGCACAGTGAGTGAACAGCTCAGGAACTCAGAAACCAAGAATCTGTGTCTC CTGGAAACAGCTCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233854 representing NM_001261453
Red=Cloning site Green=Tags(s) MEDLEETLFEEFENYSYDLDYYSLESDLEEKVQLGVVHWVSLVLYCLAFVLGIPGNAIVIWFTGFKWKKT VTTLWFLNLAIADFIFLLFLPLYISYVAMNFHWPFGIWLCKANSFTAQLNMFASVFFLTVISLDHYIHLI HPVLSHRHRTLKNSLIVIIFIWLLASLIGGPALYFRDTVEFNNHTLCYNNFQKHDPDLTLIRHHVLTWVK FIIGYLFPLLTMSICYLCLIFKVKKRSILISSRHFWTILVVVVAFVVCWTPYHLFSIWELTIHHNSYSHH VMQAGIPLSTGLAFLNSCLNPILYVLISKKFQARFRSSVAEILKYTLWEVSCSGTVSEQLRNSETKNLCL LETAQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001261453 |
| ORF Size | 1065 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001261453.1, NP_001248382.1 |
| RefSeq Size | 2581 bp |
| RefSeq ORF | 1068 bp |
| Locus ID | 2825 |
| UniProt ID | P46091 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | Receptor for the inflammation-associated leukocyte chemoattractant chemerin/RARRES2 suggesting a role for this receptor in the regulation of inflammation (PubMed:18165312). Receptor for TAFA1, mediates its effects on neuronal stem-cell proliferation and differentiation via the activation of ROCK/ERK and ROCK/STAT3 signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
