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Calumenin (CALU) (NM_001199671) Human Tagged ORF Clone
CAT＃: RG233758
- TrueORF®
CALU (tGFP-tagged) - Homo sapiens calumenin (CALU), transcript variant 3
ORF Plasmid: DDK
"NM_001199671" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233758 representing NM_001199671
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAAACTGATCTAATTATCATGGACCTGCGACAGTTTCTTATGTGCCTGTCCCTGTGCACAGCCT TTGCCTTGAGCAAACCCACAGAAAAGAAGGACCGTGTACATCATGAGCCTCAGCTCAGTGACAAGGTTCA CAATGATGCTCAGAGTTTTGATTATGACCATGATGCCTTCTTGGGTGCTGAAGAAGCAAAGACCTTTGAT CAGCTGACACCAGAAGAGAGCAAGGAAAGGCTTGGAAAGATTGTAAGTAAAATAGATGGCGACAAGGACG GGTTTGTCACTGTGGATGAGCTCAAAGACTGGATTAAATTTGCACAAAAGCGCTGGATTTACGAGGATGT AGAGCGACAGTGGAAGGGGCATGACCTCAATGAGGACGGCCTCGTTTCCTGGGAGGAGTATAAAAATGCC ACCTACGGCTACGTTTTAGATGATCCAGATCCTGATGATGGATTTAACTATAAACAGATGATGGTTAGAG ATGAGCGGAGGTTTAAAATGGCAGACAAGGATGGAGACCTCATTGCCACCAAGGAGGAGTTCACAGCTTT CCTGCACCCTGAGGAGTATGACTACATGAAAGATATAGTAGTACAGGAAACAATGGAAGATATAGATAAG AATGCTGATGGTTTCATTGATCTAGAAGAGTATATTGGTGACATGTACAGCCATGATGGGAATACTGATG AGCCAGAATGGGTAAAGACAGAGCGAGAGCAGTTTGTTGAGTTTCGGGATAAGAACCGTGATGGGAAGAT GGACAAGGAAGAGACCAAAGACTGGATCCTTCCCTCAGACTATGATCATGCAGAGGCAGAAGCCAGGCAC CTGGTCTATGAATCAGACCAAAACAAGGATGGCAAGCTTACCAAGGAGGAGATCGTTGACAAGTATGACT TATTTGTTGGCAGCCAGGCCACAGATTTTGGGGAGGCCTTAGTACGGCATGATGAGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233758 representing NM_001199671
Red=Cloning site Green=Tags(s) MKETDLIIMDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFD QLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNA TYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDK NADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARH LVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199671 |
| ORF Size | 969 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001199671.2 |
| RefSeq Size | 6181 bp |
| RefSeq ORF | 972 bp |
| Locus ID | 813 |
| UniProt ID | O43852 |
| Protein Families | Secreted Protein |
| Gene Summary | The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008] |
Documents
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Resources
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