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AP4E1 (NM_001252127) Human Tagged ORF Clone
CAT＃: RG233195
- TrueORF®
AP4E1 (tGFP-tagged) - Homo sapiens adaptor-related protein complex 4, epsilon 1 subunit (AP4E1), transcript variant 2
ORF Plasmid: DDK
"NM_001252127" in other vectors (2)
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CNY 11,690.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CPSQ4; SPG51; STUT1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233195 representing NM_001252127
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAAGGAATGTATGGTGAGACTTATATATTGTGAAATGCTTGGATATGATGCTTCCTTTGGCTATA TTCATGCAATCAAGTTAGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTAGGTTATTTGGCTGTTTCCTT ATTTCTACATGAAAGTCATGAATTATTGCTTCTCCTTGTGAATACAGTTGTAAAGGATCTGCAGAGCACT AACCTAGTAGAAGTGTGTATGGCACTGACTGTTGTTAGCCAGATTTTCCCCTGCGAAATGATTCCAGCTG TTCTTCCATTAATAGAAGATAAACTTCAACATTCTAAGGAGATTGTACGAAGAAAAGCTGTTCTGGCATT ATACAAATTCCATCTCATTGCTCCTAATCAAGTACAACATATTCATATTAAGTTTCGGAAAGCACTTTGT GACAGAGATGTTGGGGTCATGGCTGCCTCCTTGCATATATATCTTAGAATGATTAAGGAGAATTCATCTG GATATAAAGACTTGACTGGGAGTTTTGTAACCATTTTGAAGCAAGTAGTTGGAGGAAAGCTCCCAGTAGA ATTCAATTACCACAGTGTGCCAGCACCATGGTTACAAATTCAGCTCTTGAGAATACTGGGACTTCTAGGA AAAGATGATCAAAGGACAAGTGAATTAATGTATGATGTTCTTGATGAATCCTTACGAAGAGCTGAGTTAA ATCACAATGTCACATATGCTATTTTGTTTGAATGTGTGCATACAGTCTATTCTATTTATCCTAAATCGGA ATTACTTGAGAAGGCTGCCAAGTGCATTGGAAAATTTGTTCTGTCACCTAAAATAAATCTAAAATATTTA GGACTGAAGGCTCTTACCTATGTTATCCAACAGGATCCTACTCTGGCTCTTCAACACCAGATGACAATAA TTGAATGTTTAGATCATCCTGATCCCATTATTAAAAGAGAGACTCTGGAACTTCTTTACAGAATTACTAA TGCACAGAATATAACAGTTATTGTCCAGAAAATGCTTGAATATTTACATCAGAGCAAAGAAGAGTATGTC ATCGTCAATTTGGTCGGCAAAATAGCAGAGCTGGCTGAGAAATATGCTCCTGATAATGCATGGTTTATTC AGACAATGAATGCTGTGTTTTCAGTAGGAGGAGATGTAATGCATCCTGATATTCCCAATAACTTTCTGAG ACTACTAGCGGAAGGTTTTGATGATGAAACAGAAGATCAGCAATTAAGACTCTATGCAGTTCAGTCTTAT CTCACTTTACTGGATATGGAAAATGTGTTCTATCCACAGAGATTTCTTCAAGTTATGAGTTGGGTATTAG GGGAATATTCCTACCTCTTAGATAAGGAAACGCCAGAGGAAGTTATAGCTAAGCTCTACAAGTTACTTAT GAATGACTCTGTGTCTTCAGAAACAAAAGCCTGGTTAATTGCTGCTGTGACCAAATTGACATCTCAGGCG CACTCTTCTAATACAGTTGAGAGATTAATCCATGAATTTACCATATCTTTGGATACTTGTATGAGACAAC ATGCATTTGAATTAAAACATTTGCATGAGAATGTGGAACTTATGAAGAGCTTGCTTCCAGTTGACAGGAG TTGTGAAGACTTGGTGGTAGATGCTTCTTTATCTTTTCTGGATGGTTTTGTGGCTGAAGGACTCAGTCAG GGTGCAGCGCCTTACAAACCTCCCCATCAACGCCAGGAGGAAAAGCTTTCTCAGGAAAAAGTTCTCAATT TTGAACCATATGGACTCTCCTTTTCTTCATCTGGCTTCACTGGACGACAGTCTCCTGCTGGCATTTCTCT TGGTTCAGATGTATCTGGGAATAGTGCTGAGACAGGACTGAAAGAGACAAATAGCTTGAAGCTGGAAGGT ATAAAGAAATTGTGGGGGAAAGAAGGCTATCTTCCCAAGAAGGAAAGCAAAACTGGTGATGAAAGTGGAG CTCTGCCTGTTCCTCAAGAGAGTATAATGGAGAATGTAGATCAAGCTATAACTAAAAAGGATCAATCTCA AGTTCTTACCCAATCTAAAGAGGAGAAAGAAAAGCAGCTGCTGGCATCATCATTATTTGTTGGTCTAGGA TCAGAAAGTACAATCAACCTGCTGGGAAAAGCAGATACTGTCTCTCACAAGTTCAGAAGGAAATCAAAAG TCAAAGAAGCTAAAAGTGGCGAAACAACCAGTACTCATAATATGACCTGTTCTTCCTTTAGTTCTTTGTC AAATGTGGCATATGAAGATGATTATTATTCGAATACTTTGCACGATACAGGAGACAAGGAATTAAAGAAA TTTTCTCTCACTTCAGAACTTTTGGATTCTGAGTCACTCACAGAACTGCCCTTGGTTGAGAAATTCTCAT ATTGTAGTCTGTCTACACCTTCATTGTTTGCTAATAACAACATGGAAATTTTTCACCCTCCTCAATCTAC TGCAGCCTCAGTTGCCAAGGAAAGCTCTTTAGCTTCATCTTTTTTGGAAGAAACTACTGAATACATACAC TCAAATGCTATGGAAGTCTGTAATAATGAAACTATATCAGTGTCTTCTTATAAAATTTGGAAAGATGATT GTTTATTGATGGTCTGGTCAGTCACTAATAAGAGTGGTTTGGAATTGAAAAGTGCTGACTTAGAAATTTT TCCTGCAGAAAATTTCAAGGTGACTGAGCAACCTGGATGCTGTTTGCCTGTAATGGAAGCAGAAAGCACC AAAAGCTTTCAATATAGTGTGCAGATAGAAAAACCTTTTACAGAAGGAAATCTTACTGGTTTTATTAGTT ATCATATGATGGATACTCATTCTGCTCAGCTGGAATTTTCTGTAAACTTATCACTATTAGATTTCATTAG ACCATTAAAAATCTCAAGTGACGACTTTGGGAAACTCTGGTTATCCTTCGCAAATGATGTGAAACAAAAT GTAAAAATGTCAGAATCTCAAGCTGCACTTCCTTCTGCACTAAAGACTCTGCAACAGAAACTAAGACTCC ATATTATTGAGATTATAGGCAATGAAGGGCTATTGGCCTGTCAGCTGCTCCCATCCATCCCCTGCTTACT GCATTGCCGAGTTCATGCAGATGTATTAGCCCTGTGGTTCAGATCCTCCTGTTCTACTCTTCCTGACTAT TTACTGTATCAGTGTCAAAAGGTGATGGAGGGATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233195 representing NM_001252127
Red=Cloning site Green=Tags(s) MMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKDLQST NLVEVCMALTVVSQIFPCEMIPAVLPLIEDKLQHSKEIVRRKAVLALYKFHLIAPNQVQHIHIKFRKALC DRDVGVMAASLHIYLRMIKENSSGYKDLTGSFVTILKQVVGGKLPVEFNYHSVPAPWLQIQLLRILGLLG KDDQRTSELMYDVLDESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKINLKYL GLKALTYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNITVIVQKMLEYLHQSKEEYV IVNLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDDETEDQQLRLYAVQSY LTLLDMENVFYPQRFLQVMSWVLGEYSYLLDKETPEEVIAKLYKLLMNDSVSSETKAWLIAAVTKLTSQA HSSNTVERLIHEFTISLDTCMRQHAFELKHLHENVELMKSLLPVDRSCEDLVVDASLSFLDGFVAEGLSQ GAAPYKPPHQRQEEKLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDVSGNSAETGLKETNSLKLEG IKKLWGKEGYLPKKESKTGDESGALPVPQESIMENVDQAITKKDQSQVLTQSKEEKEKQLLASSLFVGLG SESTINLLGKADTVSHKFRRKSKVKEAKSGETTSTHNMTCSSFSSLSNVAYEDDYYSNTLHDTGDKELKK FSLTSELLDSESLTELPLVEKFSYCSLSTPSLFANNNMEIFHPPQSTAASVAKESSLASSFLEETTEYIH SNAMEVCNNETISVSSYKIWKDDCLLMVWSVTNKSGLELKSADLEIFPAENFKVTEQPGCCLPVMEAEST KSFQYSVQIEKPFTEGNLTGFISYHMMDTHSAQLEFSVNLSLLDFIRPLKISSDDFGKLWLSFANDVKQN VKMSESQAALPSALKTLQQKLRLHIIEIIGNEGLLACQLLPSIPCLLHCRVHADVLALWFRSSCSTLPDY LLYQCQKVMEGS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001252127 |
| ORF Size | 3186 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001252127.2 |
| RefSeq Size | 6791 bp |
| RefSeq ORF | 3189 bp |
| Locus ID | 23431 |
| UniProt ID | Q9UPM8 |
| Protein Pathways | Lysosome |
| Gene Summary | This gene encodes a member of the adaptor complexes large subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and sorting of integral membrane proteins. The encoded protein is a large subunit of adaptor protein complex-4, which is associated with both clathrin- and nonclathrin-coated vesicles. Disruption of this gene may be associated with cerebral palsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC233195 | AP4E1 (Myc-DDK tagged) - Homo sapiens adaptor-related protein complex 4, epsilon 1 subunit (AP4E1), transcript variant 2 |
CNY 10,640.00 |
|
| SC330273 | AP4E1 (untagged) - Homo sapiens adaptor-related protein complex 4, epsilon 1 subunit (AP4E1), transcript variant 2 |
CNY 10,260.00 |
