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SAP97 (DLG1) (NM_001204387) Human Tagged ORF Clone
CAT＃: RG233139
- TrueORF®
DLG1 (tGFP-tagged) - Homo sapiens discs, large homolog 1 (Drosophila) (DLG1), transcript variant 4
ORF Plasmid: DDK
"NM_001204387" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DLGH1; hdlg; SAP-97; SAP97 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233139 representing NM_001204387
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTACATTTTCGGAAACAACACACTTCTATACTCTCGCGGCAGTCGAGGAGGCAATACTAGCTCTA GCCATGGCTCAGCAGGCCCAAAGCAGAAACACTGGGCAAAAAAGGGCTCGTCAGATGAACTGCAAGCTGA GCCAGAACCTTCACGCTGGCAGCAGATAGTTGCATTTTTCACTCGAAGACACAGCTTTATTGACTGCATC TCGGTAGCCACCAGCTCCACCCAGGCAAATCCTCCCCCAGTACTGGTCAACACAGATAGCTTGGAAACAC CAACTTACGTTAATGGCACAGATGCAGATTATGAATATGAAGAAATCACACTTGAAAGGGGAAATTCAGG GCTTGGTTTCAGCATTGCAGGAGGTACGGACAACCCACACATTGGAGATGACTCAAGTATTTTCATTACC AAAATTATCACAGGGGGAGCAGCCGCCCAAGATGGAAGATTGCGGGTCAATGACTGTATATTACGAGTAA ATGAAGTAGATGTTCGTGATGTAACACATAGCAAAGCAGTTGAAGCGTTGAAAGAAGCAGGGTCTATTGT ACGCTTGTATGTAAAAAGAAGGAAACCAGTGTCAGAAAAAATAATGGAAATAAAGCTCATTAAAGGTCCT AAAGGTCTTGGGTTTAGCATTGCTGGAGGTGTTGGAAATCAGCATATTCCTGGGGATAATAGCATCTATG TAACCAAAATAATTGAAGGAGGTGCAGCACATAAGGATGGCAAACTTCAGATTGGAGATAAACTTTTAGC AGTGAATAACGTATGTTTAGAAGAAGTTACTCATGAAGAAGCAGTAACTGCCTTAAAGAACACATCTGAT TTTGTTTATTTGAAAGTGGCAAAACCCACAAGTATGTATATGAATGATGGCTATGCACCACCTGATATCA CCAACTCTTCTTCTCAGCCTGTTGATAACCATGTTAGCCCATCTTCCTTCTTGGGCCAGACACCAGCATC TCCAGCCAGATACTCCCCAGTTTCTAAAGCAGTACTTGGAGATGATGAAATTACAAGGGAACCTAGAAAA GTTGTTCTTCATCGTGGCTCAACGGGCCTTGGTTTCAACATTGTAGGAGGAGAAGATGGAGAAGGAATAT TTATTTCCTTTATCTTAGCCGGAGGACCTGCTGATCTAAGTGGAGAGCTCAGAAAAGGAGATCGTATTAT ATCGGTAAACAGTGTTGACCTCAGAGCTGCTAGTCATGAGCAGGCAGCAGCTGCATTGAAAAATGCTGGC CAGGCTGTCACAATTGTTGCACAATATCGACCTGAAGAATACAGTCGTTTTGAAGCTAAAATACATGATT TACGGGAGCAGATGATGAATAGTAGTATTAGTTCAGGGTCAGGTTCTCTTCGAACTAGCCAGAAGCGATC CCTCTATGTCAGAGCCCTTTTTGATTATGACAAGACTAAAGACAGTGGGCTTCCCAGTCAGGGACTGAAC TTCAAATTTGGAGATATCCTCCATGTTATTAATGCTTCTGATGATGAATGGTGGCAAGCCAGGCAGGTTA CACCAGATGGTGAGAGCGATGAGGTCGGAGTGATTCCCAGTAAACGCAGAGTTGAGAAGAAAGAACGAGC CCGATTAAAAACAGTGAAATTCAATTCTAAAACGAGAGATAAAGGGGAGATCCCTGACGACATGGGATCA AAAGGCCTGAAGCATGTAACTTCTAATGCCAGCGATAGTGAAAGTAGTTACCTAATCTTGATTACAGATG AGTATGGCTGCTCAAAAGGTGGTCAAGAAGAATACGTCTTATCTTATGAACCAGTGAATCAACAAGAAGT TAATTATACTCGACCAGTGATCATATTGGGACCTATGAAAGACAGGATAAATGATGACTTGATCTCAGAA TTTCCTGACAAATTTGGATCCTGTGTTCCTCATACAACTAGACCAAAACGAGATTATGAGGTAGATGGAA GAGATTATCATTTTGTGACTTCAAGAGAGCAGATGGAAAAAGATATCCAGGAACATAAATTCATTGAAGC TGGCCAGTATAACAATCATCTATATGGAACAAGTGTTCAGTCTGTACGAGAAGTAGCAGAAAAGGGCAAA CACTGTATCCTTGATGTGTCTGGAAATGCCATAAAGAGATTACAGATTGCACAGCTTTACCCTATCTCCA TTTTTATTAAACCCAAATCCATGGAAAATATCATGGAAATGAATAAGCGTCTAACAGAAGAACAAGCCAG AAAAACATTTGAGAGAGCCATGAAACTGGAACAGGAGTTTACTGAACATTTCACAGCTATTGTACAGGGG GATACGCTGGAAGACATTTACAACCAAGTGAAACAGATCATAGAAGAACAATCTGGTTCTTACATCTGGG TTCCGGCAAAAGAAAAGCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233139 representing NM_001204387
Red=Cloning site Green=Tags(s) MNYIFGNNTLLYSRGSRGGNTSSSHGSAGPKQKHWAKKGSSDELQAEPEPSRWQQIVAFFTRRHSFIDCI SVATSSTQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFIT KIITGGAAAQDGRLRVNDCILRVNEVDVRDVTHSKAVEALKEAGSIVRLYVKRRKPVSEKIMEIKLIKGP KGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNNVCLEEVTHEEAVTALKNTSD FVYLKVAKPTSMYMNDGYAPPDITNSSSQPVDNHVSPSSFLGQTPASPARYSPVSKAVLGDDEITREPRK VVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAG QAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSISSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLN FKFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRDKGEIPDDMGS KGLKHVTSNASDSESSYLILITDEYGCSKGGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRINDDLISE FPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVREVAEKGK HCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTEEQARKTFERAMKLEQEFTEHFTAIVQG DTLEDIYNQVKQIIEEQSGSYIWVPAKEKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001204387 |
| ORF Size | 2400 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001204387.1, NP_001191316.1 |
| RefSeq Size | 4798 bp |
| RefSeq ORF | 2403 bp |
| Locus ID | 1739 |
| UniProt ID | Q12959 |
| Protein Families | Druggable Genome |
| Protein Pathways | T cell receptor signaling pathway |
| Gene Summary | This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. A multitude of transcript variants deriving from alternative splicing and the use of multiple alternate promoter have been observed, including some splice variants that may be specific to brain and other tissues. An upstream uORF may regulate translation at some splice variants of this gene. [provided by RefSeq, Sep 2018] |
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