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PDE1C (NM_001191059) Human Tagged ORF Clone
CAT＃: RG233095
- TrueORF®
PDE1C (tGFP-tagged) - Homo sapiens phosphodiesterase 1C, calmodulin-dependent 70kDa (PDE1C), transcript variant 5
ORF Plasmid: DDK
"NM_001191059" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | cam-PDE 1C; DFNA74; hCam-3; Hcam3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233095 representing NM_001191059
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGCCAACCAAGGAGATTGAAGAATTTGAGAGCAACTCTCTGAAATACCTGCAACCGGAACAGA TCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACGTCCCAGAGATTACGGTCTTT GGTCAAACAATTAGAGAGAGGGGAAGCTTCAGTGGTAGATCTTAAGAAGAATTTGGAATATGCAGCCACA GTGCTTGAATCTGTGTATATTGATGAAACAAGGAGACTCCTGGATACAGAGGATGAGCTCAGTGACATTC AGTCAGATGCTGTGCCTTCTGAGGTCCGAGACTGGCTGGCCTCCACCTTCACGCGGCAGATGGGGATGAT GCTCAGGAGGAGCGACGAGAAGCCCCGGTTCAAGAGCATCGTTCACGCAGTGCAGGCTGGGATATTTGTG GAGAGAATGTATAGACGGACATCAAACATGGTTGGACTGAGCTATCCACCAGCTGTTATTGAGGCATTAA AGGATGTGGACAAGTGGTCCTTTGACGTCTTTTCCCTCAATGAGGCCAGTGGGGATCATGCACTGAAATT TATTTTCTATGAACTACTCACACGTTATGATCTGATCAGCCGTTTCAAGATCCCCATTTCTGCACTTGTC TCATTTGTGGAGGCCCTGGAAGTGGGATACAGCAAGCACAAAAATCCTTACCATAACTTAATGCACGCTG CCGATGTTACACAGACAGTGCATTACCTCCTCTATAAGACAGGAGTGGCGAACTGGCTGACGGAGCTGGA GATCTTTGCTATAATCTTCTCAGCTGCCATCCATGACTACGAGCATACCGGAACCACCAACAATTTCCAC ATTCAGACTCGGTCTGATCCAGCTATTCTGTATAATGACAGATCTGTACTGGAGAATCACCATTTAAGTG CAGCTTATCGCCTTCTGCAAGATGACGAGGAAATGAATATTTTGATTAACCTCTCAAAGGATGACTGGAG GGAGTTTCGAACCTTGGTAATTGAAATGGTGATGGCCACAGATATGTCTTGTCACTTCCAACAAATCAAA GCAATGAAGACTGCTCTGCAGCAGCCAGAAGCCATTGAAAAGCCAAAAGCCTTATCCCTTATGCTGCATA CAGCAGATATTAGCCATCCAGCAAAAGCATGGGACCTCCATCATCGCTGGACAATGTCACTCCTGGAGGA GTTCTTCAGACAGGGTGACAGAGAAGCAGAGCTGGGGCTGCCTTTTTCTCCTCTGTGTGACCGAAAGTCC ACTATGGTTGCTCAGTCACAAGTAGGTTTCATTGATTTCATCGTGGAACCCACCTTCACTGTGCTTACGG ACATGACCGAGAAGATTGTGAGTCCATTAATCGATGAAACCTCTCAAACTGGTGGGACAGGACAGAGGCG TTCGAGTTTGAATAGCATCAGCTCGTCAGATGCCAAGCGATCAGGTGTCAAGACCTCTGGTTCAGAGGGA AGTGCCCCGATCAACAATTCTGTCATCTCCGTTGACTATAAGAGCTTTAAAGCTACTTGGACGGAAGTGG TGCACATCAATCGGGAGAGATGGAGGGCCAAGGTACCCAAAGAGGAGAAGGCCAAGAAGGAAGCAGAGGA AAAGGCTCGCCTGGCCGCAGAGGAGCAGCAAAAGGAAATGGAAGCCAAAAGCCAGGCTGAAGAAGGCGCA TCTGGCAAAGCTGAGAAAAAGACGTCTGGAGAAACTAAGAATCAAGTCAATGGAACACGGGCAAACAAAA GTGACAACCCTCGTGGGAAAAACTCCAAAGCCGAGAAGTCATCAGGAGAACAGCAACAGAATGGTGACTT CAAAGATGGTAAAAATAAGACAGACAAGAAGGATCACTCTAACATCGGAAATGATTCAAAGAAAACAGAT GGCACAAAACAGCGTTCTCACGGCTCACCAGCCCCAAGCACCAGCTCCACGTGTCGCCTTACGTTGCCAG TCATCAAGCCTCCTTTGCGTCATTTTAAACGCCCTGCTTACGCATCTAGCTCCTATGCACCTTCAGTCTC AAAGAAAACTGATGAGCATCCTGCAAGGTACAAGATGCTGGATCAGAGGATCAAAATGAAAAAGATTCAG AACATCTCACATAACTGGAACAGAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233095 representing NM_001191059
Red=Cloning site Green=Tags(s) MESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAAT VLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFV ERMYRRTSNMVGLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALV SFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFH IQTRSDPAILYNDRSVLENHHLSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIK AMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKS TMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEG SAPINNSVISVDYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEMEAKSQAEEGA SGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQQQNGDFKDGKNKTDKKDHSNIGNDSKKTD GTKQRSHGSPAPSTSSTCRLTLPVIKPPLRHFKRPAYASSSYAPSVSKKTDEHPARYKMLDQRIKMKKIQ NISHNWNRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001191059 |
| ORF Size | 2127 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001191059.3 |
| RefSeq Size | 2888 bp |
| RefSeq ORF | 2130 bp |
| Locus ID | 5137 |
| UniProt ID | Q14123 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Olfactory transduction, Progesterone-mediated oocyte maturation, Purine metabolism |
| Gene Summary | This gene encodes an enzyme that belongs to the 3'5'-cyclic nucleotide phosphodiesterase family. Members of this family catalyze hydrolysis of the cyclic nucleotides, cyclic adenosine monophosphate and cyclic guanosine monophosphate, to the corresponding nucleoside 5'-monophosphates. The enzyme encoded by this gene regulates proliferation and migration of vascular smooth muscle cells, and neointimal hyperplasia. This enzyme also plays a role in pathological vascular remodeling by regulating the stability of growth factor receptors, such as PDGF-receptor-beta. [provided by RefSeq, Jul 2016] |
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