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ATP dependent metalloprotease YME1L1 (YME1L1) (NM_001253866) Human Tagged ORF Clone
CAT＃: RG233076
- TrueORF®
YME1L1 (tGFP-tagged) - Homo sapiens YME1-like 1 ATPase (YME1L1), transcript variant 4
ORF Plasmid: DDK
"NM_001253866" in other vectors (2)
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CNY 6,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FTSH; MEG4; OPA11; PAMP; YME1L |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233076 representing NM_001253866
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTCCTTGTCGAGCACGGTGCAACCCCAGGTTACAGTTCCTCTGAGTCATCTCATCAATGCCTTCC ATACACCAAAAAACACTTCTGTTTCTCTCAGTGGAGTGTCAGTTTCTCAAAACCAGCATCGAGATGTAGT TCCTGAGCATGAGGCTCCCAGCAGTGAGCCTTCACTTAACTTAAGGGACCTTGGATTATCTGAACTAAAA ATTGGACAGATTGATCAGCTGGTAGAAAATCTACTTCCTGGATTTTGTAAAGGCAAAAACATTTCTTCCC ATTGGCATACATCCCATGTCTCTGCACAATCCTTCTTTGAAAATAAATATGTTTTCATACAGTCTCGGGG TTTTAAAACTTTGAAATCAAGGACACGACGTCTCCAGTCTACCTCCGAGAGATTAGCTGAAACACAGAAT ATAGCGCCATCATTCGTGAAGGGGTTTCTTTTGCGGGACAGAGGATCAGATGTTGAGAGTTTGGACAAAC TCATGAAAACCAAAAATATACCTGAAGCTCACCAAGATGCATTTAAAACTGGTTTTGCGGAAGGTTTTCT GAAAGCTCAAGCACTCACACAAAAAACCAATGATTCCCTAAGGCGAACCCGTCTGATTCTCTTCGTTCTG CTGCTATTCGGCATTTATGGACTTCTAAAAAACCCATTTTTATCTGTCCGCTTCCGGACAACAACAGGGC TTGATTCTGCAGTAGATCCTGTCCAGATGAAAAATGTCACCTTTGAACATGTTAAAGGGGTGGAGGAAGC TAAACAAGAATTACAGGAAGTTGTTGAATTCTTGAAAAATCCACAAAAATTTACTATTCTTGGAGGTAAA CTTCCAAAAGGAATTCTTTTAGTTGGACCCCCAGGGACTGGAAAGACACTTCTTGCCCGAGCTGTGGCGG GAGAAGCTGATGTTCCTTTTTATTATGCTTCTGGATCCGAATTTGATGAGATGTTTGTGGGTGTGGGAGC CAGCCGTATCAGAAATCTTTTTAGGGAAGCAAAGGCGAATGCTCCTTGTGTTATATTTATTGATGAATTA GATTCTGTTGGTGGGAAGAGAATTGAATCTCCAATGCATCCATATTCAAGGCAGACCATAAATCAACTTC TTGCTGAAATGGATGGTTTTAAACCCAATGAAGGAGTTATCATAATAGGAGCCACAAACTTCCCAGAGGC ATTAGATAATGCCTTAATACGTCCTGGTCGTTTTGACATGCAAGTTACAGTTCCAAGGCCAGATGTAAAA GGTCGAACAGAAATTTTGAAATGGTATCTCAATAAAATAAAGTTTGATCAATCCGTTGATCCAGAAATTA TAGCTCGAGGTACTGTTGGCTTTTCCGGAGCAGAGTTGGAGAATCTTGTGAACCAGGCTGCATTAAAAGC AGCTGTTGATGGAAAAGAAATGGTTACCATGAAGGAGCTGGAGTTTTCCAAAGACAAAATTCTAATGGGG CCTGAAAGAAGAAGTGTGGAAATTGATAACAAAAACAAAACCATCACAGCATATCATGAATCTGGTCATG CCATTATTGCATATTACACAAAAGATGCAATGCCTATCAACAAAGCTACAATCATGCCACGGGGGCCAAC ACTTGGACATGTGTCCCTGTTACCTGAGAATGACAGATGGAATGAAACTAGAGCCCAGCTGCTTGCACAA ATGGATGTTAGTATGGGAGGAAGAGTGGCAGAGGAGCTTATATTTGGAACCGACCATATTACAACAGGTG CTTCCAGTGATTTTGATAATGCCACTAAAATAGCAAAGCGGATGGTTACCAAATTTGGAATGAGTGAAAA GCTTGGAGTTATGACCTACAGTGATACAGGGAAACTAAGTCCAGAAACCCAATCTGCCATCGAACAAGAA ATAAGAATCCTTCTAAGGGACTCATATGAACGAGCAAAACATATCTTGAAAACTCATGCAAAGGAGCATA AGAATCTCGCAGAAGCTTTATTGACCTATGAGACTTTGGATGCCAAAGAGATTCAAATTGTTCTTGAGGG GAAAAAGTTGGAAGTGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233076 representing NM_001253866
Red=Cloning site Green=Tags(s) MFSLSSTVQPQVTVPLSHLINAFHTPKNTSVSLSGVSVSQNQHRDVVPEHEAPSSEPSLNLRDLGLSELK IGQIDQLVENLLPGFCKGKNISSHWHTSHVSAQSFFENKYVFIQSRGFKTLKSRTRRLQSTSERLAETQN IAPSFVKGFLLRDRGSDVESLDKLMKTKNIPEAHQDAFKTGFAEGFLKAQALTQKTNDSLRRTRLILFVL LLFGIYGLLKNPFLSVRFRTTTGLDSAVDPVQMKNVTFEHVKGVEEAKQELQEVVEFLKNPQKFTILGGK LPKGILLVGPPGTGKTLLARAVAGEADVPFYYASGSEFDEMFVGVGASRIRNLFREAKANAPCVIFIDEL DSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVIIIGATNFPEALDNALIRPGRFDMQVTVPRPDVK GRTEILKWYLNKIKFDQSVDPEIIARGTVGFSGAELENLVNQAALKAAVDGKEMVTMKELEFSKDKILMG PERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPINKATIMPRGPTLGHVSLLPENDRWNETRAQLLAQ MDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKRMVTKFGMSEKLGVMTYSDTGKLSPETQSAIEQE IRILLRDSYERAKHILKTHAKEHKNLAEALLTYETLDAKEIQIVLEGKKLEVR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001253866 |
| ORF Size | 2049 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001253866.1, NP_001240795.1 |
| RefSeq Size | 4133 bp |
| RefSeq ORF | 2052 bp |
| Locus ID | 10730 |
| UniProt ID | Q96TA2 |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
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| FAQs |
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Resources
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