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PLRG1 (NM_001201564) Human Tagged ORF Clone
CAT＃: RG232881
- TrueORF®
PLRG1 (tGFP-tagged) - Homo sapiens pleiotropic regulator 1 (PLRG1), transcript variant 2
ORF Plasmid: DDK
"NM_001201564" in other vectors (2)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cwc1; PRL1; PRP46; PRPF46; TANGO4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232881 representing NM_001201564
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCGAGGAGGTACAGAAACATTCTGTACACACCCTTGTGTTCAGGTCGTTGAAGAGGACCCATGACA TGTTTGTAGCTGATAATGGAAAACCTGTGCCTTTAGATGAAGAGAGTCACAAACGAAAAATGGCAATCAA GCTTCGTAATGAGTATGGTCCTGTGTTGCATATGCCTACTTCAAAAGAAAATCTTAAAGAGAAGGGTCCT CAGAATGCAACGGATTCATATGTTCATAAACAGTACCCTGCCAATCAAGGACAAGAAGTTGAATACTTTG TGGCAGGGGTTGCTTTGACAGCAGATACTAAGATCCAGAGAATGCCAAGTGAATCAGCTGCACAGTCCTT AGCGGTGGCATTACCTTTGCAGACCAAGGCTGATGCAAATCGTACTGCCCCTAGTGGAAGTGAATACCGA CATCCTGGGGCTTCTGACCGTCCACAGCCTACAGCGATGAATTCAATTGTCATGGAGACTGGCAATACCA AGAACTCTGCACTGATGGCTAAAAAAGCCCCTACAATGCCAAAACCCCAGTGGCACCCACCGTGGAAACT CTACAGGGTTATCAGTGGGCATCTTGGCTGGGTTCGATGTATTGCTGTGGAACCTGGAAATCAGTGGTTT GTTACTGGATCTGCTGACAGAACTATAAAGATCTGGGACTTGGCTAGTGGCAAATTAAAACTGTCATTGA CTGGGCATATTAGTACTGTGCGGGGCGTGATAGTAAGCACAAGGAGCCCATATCTGTTCTCTTGTGGAGA AGACAAACAAGTGAAATGCTGGGATCTCGAATACAATAAGGTTATACGGCATTATCATGGACATTTAAGT GCAGTGTATGGTTTGGATTTGCACCCGACAATCGATGTGTTGGTAACCTGTAGTCGAGATTCAACTGCAC GGATTTGGGATGTGAGAACTAAAGCCAGTGTACACACATTATCTGGACATACAAATGCAGTTGCTACAGT GAGATGTCAGGCTGCAGAACCACAAATTATTACAGGAAGCCATGATACTACAATTCGATTATGGGATCTG GTGGCTGGAAAAACAAGAGTGACATTAACAAATCACAAAAAATCAGTTAGGGCTGTGGTTTTACATCCAA GACATTACACATTTGCATCTGGTTCTCCAGATAACATAAAGCAGTGGAAATTCCCTGATGGAAGTTTCAT TCAAAATCTTTCCGGTCATAATGCTATTATTAACACATTGACGGTAAATTCTGATGGAGTGCTTGTATCT GGAGCTGACAATGGCACCATGCATCTTTGGGACTGGAGAACTGGCTACAATTTTCAGAGAGTTCACGCAG CTGTGCAACCTGGGTCTTTGGACAGTGAATCAGGAATATTTGCTTGTGCTTTTGATCAGTCTGAAAGTCG ATTACTAACAGCTGAAGCTGATAAAACCATTAAAGTATACAGAGAGGATGACACAGCCACAGAAGAAACT CATCCAGTCAGCTGGAAACCAGAAATTATCAAGAGAAAGAGATTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232881 representing NM_001201564
Red=Cloning site Green=Tags(s) MVEEVQKHSVHTLVFRSLKRTHDMFVADNGKPVPLDEESHKRKMAIKLRNEYGPVLHMPTSKENLKEKGP QNATDSYVHKQYPANQGQEVEYFVAGVALTADTKIQRMPSESAAQSLAVALPLQTKADANRTAPSGSEYR HPGASDRPQPTAMNSIVMETGNTKNSALMAKKAPTMPKPQWHPPWKLYRVISGHLGWVRCIAVEPGNQWF VTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLS AVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSHDTTIRLWDL VAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLTVNSDGVLVS GADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFACAFDQSESRLLTAEADKTIKVYREDDTATEET HPVSWKPEIIKRKRF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001201564 |
| ORF Size | 1515 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001201564.1, NP_001188493.1 |
| RefSeq Size | 3347 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 5356 |
| UniProt ID | O43660 |
| Protein Families | Transcription Factors |
| Protein Pathways | Spliceosome |
| Gene Summary | This gene encodes a core component of the cell division cycle 5-like (CDC5L) complex. The CDC5L complex is part of the spliceosome and is required for pre-mRNA splicing. The encoded protein plays a critical role in alternative splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
Documents
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