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GPR161 (NM_001267614) Human Tagged ORF Clone
CAT＃: RG232681
- TrueORF®
GPR161 (tGFP-tagged) - Homo sapiens G protein-coupled receptor 161 (GPR161), transcript variant 7
ORF Plasmid: DDK
"NM_001267614" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RE2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232681 representing NM_001267614
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGTGCCCGTCGGCTGGATGAATGAAAGCTACTATGCTGTCCTGTACCCCATGGTGTACCCCATGA AGATCACAGGGAACCGGGCTGTGATGGCACTTGTCTACATCTGGCTTCACTCGCTCATCGGCTGCCTGCC ACCCCTGTTTGGTTGGTCATCCGTGGAGTTTGACGAGTTCAAATGGATGTGTGTGGCTGCTTGGCACCGG GAGCCTGGCTACACGGCCTTCTGGCAGATCTGGTGTGCCCTCTTCCCCTTTCTGGTCATGCTGGTGTGCT ATGGCTTCATCTTCCGCGTGGCCAGGGTCAAGGCACGCAAGGTGCACTGTGGCACAGTCGTCATCGTGGA GGAGGATGCTCAGAGGACCGGGAGGAAGAACTCCAGCACCTCCACCTCCTCTTCAGGCAGCAGGAGGAAT GCCTTTCAGGGTGTGGTCTACTCGGCCAACCAGTGCAAAGCCCTCATCACCATCCTGGTGGTCCTCGGTG CCTTCATGGTCACCTGGGGCCCCTACATGGTTGTCATCGCCTCTGAGGCCCTCTGGGGGAAAAGCTCCGT CTCCCCGAGCCTGGAGACTTGGGCCACATGGCTGTCCTTTGCCAGCGCTGTCTGCCACCCCCTGATCTAT GGACTCTGGAACAAGACAGTTCGCAAAGAACTACTGGGCATGTGCTTTGGGGACCGGTATTATCGGGAAC CATTTGTGCAACGACAGAGGACTTCCAGGCTCTTCAGCATTTCCAACAGGATCACAGACCTGGGCCTGTC CCCACACCTCACTGCGCTCATGGCAGGTGGACAGCCCCTGGGGCACAGCAGCAGCACGGGGGACACTGGC TTCAGCTGCTCCCAGGACTCAGGGACAGATATGATGCTGCTTGAGGACTACACGTCTGATGACAACCCTC CCTCTCACTGCACTTGCCCACCCAAGAGAAGGAGCTCGGTGACATTTGAGGATGAAGTGGAACAAATCAA AGAAGCTGCCAAGAACTCGATTCTTCATGTGAAAGCTGAAGTACACAAGTCCTTGGACAGTTACGCAGCA AGCTTGGCCAAAGCCATTGAGGCCGAAGCCAAAATCAACTTATTTGGGGAGGAGGCTTTGCCAGGGGTCT TGGTTACAGCACGGACTGTCCCGGGGGGCGGCTTCGGGGGCCGCCGAGGCAGCAGAACTCTTGTGAGCCA GAGGCTGCAGTTGCAGAGCATCGAAGAAGGAGATGTTTTAGCTGCCGAGCAGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232681 representing NM_001267614
Red=Cloning site Green=Tags(s) MLVPVGWMNESYYAVLYPMVYPMKITGNRAVMALVYIWLHSLIGCLPPLFGWSSVEFDEFKWMCVAAWHR EPGYTAFWQIWCALFPFLVMLVCYGFIFRVARVKARKVHCGTVVIVEEDAQRTGRKNSSTSTSSSGSRRN AFQGVVYSANQCKALITILVVLGAFMVTWGPYMVVIASEALWGKSSVSPSLETWATWLSFASAVCHPLIY GLWNKTVRKELLGMCFGDRYYREPFVQRQRTSRLFSISNRITDLGLSPHLTALMAGGQPLGHSSSTGDTG FSCSQDSGTDMMLLEDYTSDDNPPSHCTCPPKRRSSVTFEDEVEQIKEAAKNSILHVKAEVHKSLDSYAA SLAKAIEAEAKINLFGEEALPGVLVTARTVPGGGFGGRRGSRTLVSQRLQLQSIEEGDVLAAEQR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001267614 |
| ORF Size | 1245 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001267614.1, NP_001254543.1 |
| RefSeq Size | 7397 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 23432 |
| UniProt ID | Q8N6U8 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. [provided by RefSeq, Mar 2017] |
Documents
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