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DCTN1 (NM_001190837) Human Tagged ORF Clone
CAT＃: RG231480
- TrueORF®
DCTN1 (tGFP-tagged) - Human dynactin 1 (DCTN1), transcript variant 6
ORF Plasmid: DDK
"NM_001190837" in other vectors (3)
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CNY 13,870.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DAP-150; DP-150; P135 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG231480 representing NM_001190837
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAGAGCAAGAGGCACGTGTACAGCCGGACGCCCAGCGGCAGCAGGATGAGTGCGGAGGCAAGCG CCCGGCCTCTGCGGGTGGGCTCCCGTGTAGAGGTGATTGGAAAAGGCCACCGAGGCACTGTGGCCTATGT TGGAGCCACACTGTTTGCCACTGGCAAATGGGTAGGCGTGATTCTGGATGAAGCAAAGGGCAAAAATGAT GGAACTGTTCAAGGCAGGAAGTACTTCACTTGTGATGAAGGGCATGGCATCTTTGTGCGCCAGTCCCAGA TCCAGGTATTTGAAGATGGAGCAGATACTACTTCCCCAGAGACACCTGATTCTTCTGCTTCAAAAGTCCT CAAAAGAGAGGGAACTGATACAACTGCAAAGACTAGCAAACTGGCACCGACAGCCCGAAAGACCACAACT CGGCGACCCAAGCCCACGCGCCCAGCCAGTACTGGGGTGGCTGGGGCCAGTAGCTCCCTGGGCCCCTCTG GCTCAGCGTCAGCAGGTGAGCTGAGCAGCAGTGAGCCCAGCACCCCGGCTCAGACTCCGCTGGCAGCACC CATCATCCCCACGCCGGTCCTCACCTCTCCTGGAGCAGTCCCCCCGCTTCCTTCCCCATCCAAGGAGGAG GAGGGACTAAGGGCTCAGGTGCGGGACCTGGAGGAGAAACTAGAGACCCTGAGACTGAAACGGGCAGAAG ACAAAGCAAAGCTAAAAGAGCTGGAGAAACACAAAATCCAGCTGGAGCAGGTGCAGGAATGGAAGAGCAA AATGCAGGAGCAGCAGGCCGACCTGCAGCGGCGCCTCAAGGAGGCGAGAAAGGAAGCCAAGGAGGCGCTG GAGGCAAAGGAACGCTATATGGAGGAGATGGCTGATACTGCTGATGCCATTGAGATGGCCACTTTGGACA AGGAGATGGCTGAAGAGCGGGCTGAGTCCCTGCAGCAGGAGGTGGAGGCACTGAAGGAGCGGGTGGACGA GCTCACTACTGACTTAGAGATCCTCAAGGCTGAGATTGAAGAGAAGGGCTCAGATGGCGCTGCATCCAGT TATCAGCTCAAGCAGCTTGAGGAGCAGAATGCCCGCCTGAAGGATGCCCTGGTGAGGATGCGGGATCTTT CTTCCTCAGAGAAGCAGGAGCATGTGAAGCTCCAGAAGCTCATGGAAAAGAAGAACCAAGAGCTGGAAGT TGTGAGGCAACAGCGGGAGCGTCTGCAGGAGGAGCTAAGCCAGGCAGAGAGCACCATTGATGAGCTCAAG GAGCAGGTGGATGCTGCTCTGGGTGCTGAGGAGATGGTGGAGATGCTGACAGATCGGAACCTGAATCTGG AAGAGAAAGTGCGCGAGTTGAGGGAGACTGTGGGAGACTTGGAAGCGATGAATGAGATGAACGATGAGCT GCAGGAGAATGCACGTGAGACAGAACTGGAGCTGCGGGAGCAGCTGGACATGGCAGGCGCGCGGGTTCGT GAGGCCCAGAAGCGTGTGGAGGCAGCCCAGGAGACGGTTGCAGACTACCAGCAGACCATCAAGAAGTACC GCCAGCTGACCGCCCATCTACAGGATGTGAATCGGGAACTGACAAACCAGCAGGAAGCATCTGTGGAGAG GCAACAGCAGCCACCTCCAGAGACCTTTGACTTCAAAATCAAGTTTGCTGAGACTAAGGCCCATGCCAAG GCAATTGAGATGGAATTGAGGCAGATGGAGGTGGCCCAGGCCAATCGACACATGTCCCTGCTGACAGCCT TCATGCCTGACAGCTTCCTTCGGCCAGGTGGGGACCATGACTGCGTTCTGGTGCTGTTGCTCATGCCTCG TCTCATTTGCAAGGCAGAGCTGATCCGGAAGCAGGCCCAGGAGAAGTTTGAACTAAGTGAGAACTGTTCA GAGCGGCCTGGGCTGCGAGGAGCTGCTGGGGAGCAACTCAGCTTTGCTGCTGGACTGGTGTACTCGCTGA GCCTGCTGCAGGCCACGCTACACCGCTATGAGCATGCCCTCTCTCAGTGCAGTGTGGATGTGTATAAGAA AGTGGGCAGCCTGTACCCTGAGATGAGTGCCCATGAGCGCTCCTTGGATTTCCTCATTGAACTGCTGCAC AAGGATCAGCTGGATGAGACTGTCAATGTGGAGCCTCTCACCAAGGCCATCAAGTACTATCAGCATCTGT ACAGCATCCACCTTGCCGAACAGCCTGAGGACTGTACTATGCAGCTGGCTGACCACATTAAGTTCACGCA GAGTGCTCTGGACTGCATGAGTGTGGAGGTAGGACGGCTGCGTGCCTTCTTGCAGGGTGGGCAGGAGGCT ACAGATATTGCCCTCCTGCTCCGGGATCTGGAAACTTCATGCAGTGACATCCGCCAGTTCTGCAAGAAGA TCCGAAGGCGAATGCCAGGGACAGATGCTCCTGGGATCCCAGCTGCACTGGCCTTTGGACCACAGGTATC TGACACGCTCCTAGACTGCAGGAAACACTTGACGTGGGTCGTGGCTGTGCTGCAGGAGGTGGCAGCTGCT GCTGCCCAGCTCATTGCCCCACTGGCAGAGAATGAGGGGCTACTTGTGGCTGCTCTGGAGGAACTGGCTT TCAAAGCAAGCGAGCAGATCTATGGGACCCCCTCCAGCAGCCCCTATGAGTGTCTGCGCCAGTCATGCAA CATCCTCATCAGTACCATGAACAAGCTGGCCACAGCCATGCAGGAGGGGGAGTATGATGCAGAGCGGCCC CCCAGCAAGCCTCCACCGGTTGAACTGCGGGCTGCTGCCCTTCGTGCAGAGATCACAGATGCTGAAGGCC TGGGTTTGAAGCTCGAAGATCGAGAGACAGTTATTAAGGAGTTGAAGAAGTCACTCAAGATTAAGGGAGA GGAGCTAAGTGAGGCCAATGTGCGGCTGAGCCTCCTGGAGAAGAAGTTGGACAGTGCTGCCAAGGATGCA GATGAGCGCATCGAGAAAGTCCAGACTCGGCTGGAGGAGACCCAGGCACTGCTGCGAAAGAAGGAGAAAG AGTTTGAGGAGACAATGGATGCACTCCAGGCTGACATCGACCAGCTGGAGGCAGAGAAGGCAGAACTAAA GCAGCGTCTGAACAGCCAGTCCAAACGCACGATTGAGGGACTCCGGGGCCCTCCTCCTTCAGGCATTGCT ACTCTGGTCTCTGGCATTGCTGGTGAAGAACAGCAGCGAGGAGCCATCCCTGGGCAGGCTCCAGGGTCTG TGCCAGGCCCAGGGCTGGTGAAGGACTCACCACTGCTGCTTCAGCAGATCTCTGCCATGAGGCTGCACAT CTCCCAGCTCCAGCATGAGAACAGCATCCTCAAGGGAGCCCAGATGAAGGCATCCTTGGCATCCCTGCCC CCTCTGCATGTTGCAAAGCTATCCCATGAGGGCCCTGGCAGTGAGTTACCAGCTGGAGCGCTGTATCGTA AGACCAGCCAGCTGCTGGAGACATTGAATCAATTGAGCACACACACGCACGTAGTAGACATCACTCGCAC CAGCCCTGCTGCCAAGAGCCCGTCGGCCCAACTTATGGAGCAAGTGGCTCAGCTTAAGTCCCTGAGTGAC ACCGTCGAGAAGCTCAAGGATGAGGTCCTCAAGGAGACAGTATCTCAGCGCCCTGGAGCCACAGTACCCA CTGACTTTGCCACCTTCCCTTCATCAGCCTTCCTCAGGGCCAAGGAGGAGCAGCAGGATGACACAGTCTA CATGGGCAAAGTGACCTTCTCATGTGCGGCTGGTTTTGGACAGCGACACCGGCTGGTGCTGACCCAGGAG CAGCTGCACCAGCTTCACAGTCGCCTCATCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231480 representing NM_001190837
Red=Cloning site Green=Tags(s) MAQSKRHVYSRTPSGSRMSAEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKND GTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGTDTTAKTSKLAPTARKTTT RRPKPTRPASTGVAGASSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSKEE EGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEAL EAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASS YQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELK EQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVR EAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAK AIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFELSENCS ERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLH KDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDCTMQLADHIKFTQSALDCMSVEVGRLRAFLQGGQEA TDIALLLRDLETSCSDIRQFCKKIRRRMPGTDAPGIPAALAFGPQVSDTLLDCRKHLTWVVAVLQEVAAA AAQLIAPLAENEGLLVAALEELAFKASEQIYGTPSSSPYECLRQSCNILISTMNKLATAMQEGEYDAERP PSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDA DERIEKVQTRLEETQALLRKKEKEFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIA TLVSGIAGEEQQRGAIPGQAPGSVPGPGLVKDSPLLLQQISAMRLHISQLQHENSILKGAQMKASLASLP PLHVAKLSHEGPGSELPAGALYRKTSQLLETLNQLSTHTHVVDITRTSPAAKSPSAQLMEQVAQLKSLSD TVEKLKDEVLKETVSQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGFGQRHRLVLTQE QLHQLHSRLIS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001190837 |
| ORF Size | 3813 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001190837.2 |
| RefSeq Size | 4497 bp |
| RefSeq ORF | 3816 bp |
| Locus ID | 1639 |
| UniProt ID | Q14203 |
| Protein Families | Druggable Genome |
| Protein Pathways | Huntington's disease |
| Gene Summary | This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231480 | DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 6 |
CNY 12,640.00 |
|
| RC231480L3 | Lenti-ORF clone of DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 6 |
CNY 14,540.00 |
|
| RC231480L4 | Lenti-ORF clone of DCTN1 (mGFP-tagged)-Human dynactin 1 (DCTN1), transcript variant 6 |
CNY 14,540.00 |
