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Cullin 2 (CUL2) (NM_001198778) Human Tagged ORF Clone
CAT＃: RG231406
- TrueORF®
CUL2 (tGFP-tagged) - Human cullin 2 (CUL2), transcript variant 1
ORF Plasmid: DDK
"NM_001198778" in other vectors (3)
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CNY 8,360.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG231406 representing NM_001198778
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAGAGTAACATGGTCAACTTTTTGGCTTAGATTTCAACACTACACTTGCACAATGTCTTTGAAAC CAAGAGTAGTAGATTTTGATGAAACATGGAACAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGA ATACGTCGAAAGAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAA CCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTTGGAAAATCATGTTCGGCATTTGCATAAGA GAGTTTTGGAGTCAGAAGAACAAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGC AGACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAAGAATAAATTAACAGAAGCG GACCTTCAGTATGGCTATGGTGGTGTAGATATGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGG ATATGTGGAGGAAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCGAGAAATCAA AAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTT GAACAGTATAAGAAAAAATTCCCCTTAAAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAA CAGGAGAGTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACTGCTCACAGTATATGGAAAA GGTTCTAGGTAGATTAAAAGATGAAGAAATTCGATGTCGAAAATACCTACATCCAAGTTCATATACTAAG GTGATTCATGAATGTCAACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAATGTCATAATA TAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTACGTCTTACTCCGTGCTGTGTCCACTGGTTT ACCTCATATGATTCAGGAGCTGCAAAACCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACT CAGGAAAACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGTAAATTTGTTCAGCTTATCA ACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGCGTTGGATAAGGCCCTTACGTCAGTTGTAAATTA CAGAGAACCTAAGTCTGTTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTACTGAAGAAG TCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCTCACGAGCTTCATCACAGTGTTCAAATACA TTGATGACAAGGACGTCTTTCAAAAGTTCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATC CATGTCTATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTGTGGTTATGAGTTTACCAGC AAGCTACATCGGATGTATACAGATATGAGTGTCAGCGCTGATCTCAACAATAAGTTCAACAATTTTATCA AAAACCAAGACACAGTAATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGGTGCGTGGCC TCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGGAATTAGAAAAAAGTGTACAGATGTTTGAA TTATTTTATAGCCAACATTTCAGTGGAAGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTA AAATGAACTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAATGGCAGTTCTTCTTGCCTT TAACAACAGTGAAACTGTCAGTTATAAAGAGCTTCAGGACAGCACTCAGATGAATGAAAAGGAACTGACA AAAACAATCAAATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATATTGATGCAG AATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGAACAAAATTTAAAATTACTACATCAATGCA GAAAGACACACCACAAGAAATGGAGCAGACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAA GCTGCTATAGTTCGTATCATGAAAGCACGAAAAGTGCTTCGGCACAATGCCCTTATTCAAGAGGTGATTA GCCAGTCAAGAGCTAGGTTTAATCCCAGTATCAGCATGATTAAGAAGTGTATTGAAGTTCTGATAGACAA ACAATACATAGAACGCAGCCAGGCGTCGGCAGATGAATACAGCTACGTCGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231406 representing NM_001198778
Red=Cloning site Green=Tags(s) MYRVTWSTFWLRFQHYTCTMSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPE PLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEA DLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHV EQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTK VIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLT QENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKK SAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTS KLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFE LFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELT KTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQ AAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001198778 |
| ORF Size | 2292 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001198778.2 |
| RefSeq Size | 4172 bp |
| RefSeq ORF | 2295 bp |
| Locus ID | 8453 |
| UniProt ID | Q13617 |
| Protein Families | Druggable Genome |
| Protein Pathways | Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis |
| Gene Summary | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231406 | CUL2 (Myc-DDK-tagged)-Human cullin 2 (CUL2), transcript variant 1 |
CNY 7,600.00 |
|
| RC231406L3 | Lenti-ORF clone of CUL2 (Myc-DDK-tagged)-Human cullin 2 (CUL2), transcript variant 1 |
CNY 9,500.00 |
|
| RC231406L4 | Lenti-ORF clone of CUL2 (mGFP-tagged)-Human cullin 2 (CUL2), transcript variant 1 |
CNY 9,500.00 |
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