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POLE2 (NM_001197331) Human Tagged ORF Clone
CAT＃: RG231277
- TrueORF®
POLE2 (tGFP-tagged) - Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 3
ORF Plasmid: DDK
"NM_001197331" in other vectors (3)
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CNY 4,850.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DPE2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG231277 representing NM_001197331
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGGAGCGGCTGCGGAGCCGGGCGCTCTCCGCCTTCAAGTTGCGGGGCTTGCTGCTCCGTGGTG AAGCTATTAAGTACCTCACAGAAGCTCTTCAGTCTATCAGTGAATTAGAGCTTGAAGATAAACTGGAAAA GATAATTAATGCAGTTGAGAAGCAACCCTTGTCATCAAACATGATTGAACGATCTGTGGTGGAAGCAGCA GTCCAGGAATGCAGTCAGTCTGTTGATGAAACTATAGAGCACGTTTTCAATATCATAGGAGCATTTGATA TTCCACGCTTTGTGTACAATTCAGAAAGAAAAAAATTTCTTCCTCTGTTAATGACCAACCACCCTGCACC AAATTTATTTGGAACACCAAGAGATAAAGCAGAGATGTTTCGTGAGCGATATACCATTTTGCACCAGAGG ACCCACAGGCATGAATTATTTACTCCTCCGGTGATAGGTTCTCACCCTGATGAAAGCGGAAGCAAATTCC AGCTTAAAACAATAGAAACCTTATTGGGTAGTACAACCAAAATCGGAGATGCGATTGTTCTTGGAATGAT AACGCAGTTAAAAGAGGGAAAATTTTTTCTGGAAGATCCTACTGGAACAGTCCAACTAGACCTTAGTAAA GCTCAGTTCCATAGTGGTTTATACACAGAGGCATGCTTTGTCTTAGCAGAAGGTTGGTTTGAAGATCAAG TGTTTCATGTCAATGCCTTTGGATTTCCACCCACTGAGCCCTCTAGTACTACTAGGGCATACTATGGAAA TATTAATTTTTTTGGAGGTCCTTCTAATACATCTGTGAAGACTTCTGCAAAACTAAAACAGCTAGAAGAG GAGAATAAAGATGCTATGTTTGTGTTTTTATCTGATGTTTGGTTGGACCAGGTGGAAGTATTGGAAAAAC TTCGCATAATGTTTGCTGGTTATTCACCAGCACCTCCAACCTGCTTTATTCTGTGTGGTAATTTTTCATC TGCACCATATGGAAAAAATCAAGTTCAAGCTTTGAAAGATTCCCTAAAAACTTTGGCAGATATAATATGT GAATACCCAGATATTCACCAAAGTAGTCGTTTTGTGTTTGTACCTGGTCCAGAGGATCCTGGATTTGGTT CCATCTTACCAAGGCCACCACTTGCTGAAAGCATCACTAATGAATTCAGACAAAGGGTACCATTTTCAGT TTTTACTACTAATCCTTGCAGAATTCAGTACTGTACACAGGAAATTACTGTCTTCCGTGAAGACTTAGTA AATAAAATGTGCAGAAACTGCGTCCGTTTTCCTAGCAGCAATTTGGCTATTCCTAATCACTTTGTAAAGA CTATCTTATCCCAAGGACATCTGACTCCCCTACCTCTTTATGTCTGCCCAGTGTATTGGGCATATGACTA TGCTTTGAGAGTGTATCCTGTGCCCGATCTACTTGTCATTGCAGACAAATATGATCCTTTCACTACGACA AATACCGAATGCCTCTGCATAAACCCTGTAAGAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231277 representing NM_001197331
Red=Cloning site Green=Tags(s) MAPERLRSRALSAFKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSNMIERSVVEAA VQECSQSVDETIEHVFNIIGAFDIPRFVYNSERKKFLPLLMTNHPAPNLFGTPRDKAEMFRERYTILHQR THRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTTKIGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSK AQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEE ENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKTLADIIC EYPDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLV NKMCRNCVRFPSSNLAIPNHFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTT NTECLCINPVRM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001197331 |
| ORF Size | 1506 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001197331.2 |
| RefSeq Size | 1782 bp |
| RefSeq ORF | 1509 bp |
| Locus ID | 5427 |
| UniProt ID | P56282 |
| Protein Pathways | Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism |
| Gene Summary | DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency. [provided by RefSeq, Jan 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231277 | POLE2 (Myc-DDK-tagged)-Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 3 |
CNY 3,736.00 |
|
| RC231277L3 | Lenti-ORF clone of POLE2 (Myc-DDK-tagged)-Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 3 |
CNY 6,270.00 |
|
| RC231277L4 | Lenti-ORF clone of POLE2 (mGFP-tagged)-Human polymerase (DNA directed), epsilon 2 (p59 subunit) (POLE2), transcript variant 3 |
CNY 6,270.00 |
