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Pyrin (MEFV) (NM_001198536) Human Tagged ORF Clone
CAT＃: RG231238
- TrueORF®
MEFV (tGFP-tagged) - Human Mediterranean fever (MEFV), transcript variant 2
ORF Plasmid: DDK
"NM_001198536" in other vectors (3)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FMF; MEF; PAAND; TRIM20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG231238 representing NM_001198536
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAAGACCCCTAGTGACCATCTGCTGTCCACCCTGGAGGAGCTGGTGCCCTATGACTTCGAGAAGT TCAAGTTCAAGCTGCAGAACACCAGTGTGCAGAAGGAGCACTCCAGGATCCCCCGGAGCCAGATCCAGAG AGCCAGGCCGGTGAAGATGGCCACTCTGCTGGTCACCTACTATGGGGAAGAGTACGCCGTGCAGCTCACC CTGCAGGTCCTGCGGGCCATCAACCAGCGCCTGCTGGCCGAGGAGCTCCACAGGGCAGCCATTCAGGGAA GGCCACCAGACACGGCTGCGAGTCCCCGCTGCCACGCCCAGGAAGGAGACCCAGTTGACGGTACCTGTGT GCGTGATTCCTGCAGCTTCCCCGAGGCAGTTTCTGGGCACCCCCAGGCCTCAGGCAGCCGCTCACCTGGC TGCCCCCGGTGCCAGGACTCCCATGAAAGGAAGAGCCCGGGAAGCCTAAGCCCCCAGCCCCTGCCACAGT GTAAGCGCCACCTGAAGCAGGTCCAGCTGCTCTTCTGTGAGGATCACGATGAGCCCATCTGCCTCATCTG CAGTCTGAGTCAGGAGCACCAAGGCCACCGGGTGCGCCCCATTGAGGAGGTCGCCCTGGAACACAAGAAG AAAATTCAGAAGCAGCTGGAGCATCTGAAGAAGCTGAGAAAATCAGGGGAGGAGCAGCGATCCTATGGGG AGGAGAAGGCAGTGAGCTTTCTGAAACAAACTGAAGCGCTGAAGCAGCGGGTGCAGAGGAAGCTGGAGCA GGTGTACTACTTCCTGGAGCAGCAAGAGCATTTCTTTGTGGCCTCACTGGAGGACGTGGGCCAGATGGTT GGGCAGATCAGGAAGGCATATGACACCCGCGTATCCCAGGACATCGCCCTGCTCGATGCGCTGATTGGGG AACTGGAGGCCAAGGAGTGCCAGTCAGAATGGGAACTTCTGCAGGACATTGGAGACATCTTGCACAGGGC TAAGACAGTGCCTGTCCCTGAAAAGTGGACCACTCCTCAAGAGATAAAACAAAAGATCCAACTCCTCCAC CAGAAGTCAGAGTTTGTGGAGAAGAGCACAAAGTACTTCTCAGAAACCCTGCGTTCAGAAATGGAAATGT TCAATGACCACAGTCCCCAACATGGCCTGGGTTCCTGGGAGGAACGGGATTATACCCAACATAGCATGCA GGGCCCTAAGCAGGGGGTTCCTTGTCTTTCCTTGTTGTCAGGACAGTGTAATTTAGCCCCTCTTAATGCT AATGCTCAGGATTTTTTTCCCTATCTGATTTTTCTCCGTAGTTCCAGAGCTGATTGGCGCTCAGGCACAT GCTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231238 representing NM_001198536
Red=Cloning site Green=Tags(s) MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLT LQVLRAINQRLLAEELHRAAIQGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSPG CPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHKK KIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQMV GQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLLH QKSEFVEKSTKYFSETLRSEMEMFNDHSPQHGLGSWEERDYTQHSMQGPKQGVPCLSLLSGQCNLAPLNA NAQDFFPYLIFLRSSRADWRSGTCC TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001198536 |
| ORF Size | 1335 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001198536.1, NP_001185465.1 |
| RefSeq Size | 3049 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 4210 |
| UniProt ID | O15553 |
| Protein Families | Druggable Genome |
| Protein Pathways | NOD-like receptor signaling pathway |
| Gene Summary | This gene encodes a protein, also known as pyrin or marenostrin, that is an important modulator of innate immunity. Mutations in this gene are associated with Mediterranean fever, a hereditary periodic fever syndrome. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231238 | MEFV (Myc-DDK-tagged)-Human Mediterranean fever (MEFV), transcript variant 2 |
CNY 3,656.00 |
|
| RC231238L3 | Lenti ORF clone of Human Mediterranean fever (MEFV), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC231238L4 | Lenti ORF clone of Human Mediterranean fever (MEFV), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
