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Cytochrome P450 2C8 (CYP2C8) (NM_001198854) Human Tagged ORF Clone
CAT＃: RG231201
- TrueORF®
CYP2C8 (tGFP-tagged) - Human cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), transcript variant 3
ORF Plasmid: DDK
"NM_001198854" in other vectors (3)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CPC8; CYP2C8DM; CYPIIC8; MP-12/MP-20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG231201 representing NM_001198854
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTCCAGCCAATTGCAAAGGGAATCATTTCCAGCAATGGAAAGAGATGGAAGGAGATCCGGCGTT TCTCCCTCACAACCTTGCGGAATTTTGGGATGGGGAAGAGGAGCATTGAGGACCGTGTTCAAGAGGAAGC TCACTGCCTTGTGGAGGAGTTGAGAAAAACCAAGGCTTCACCCTGTGATCCCACTTTCATCCTGGGCTGT GCTCCCTGCAATGTGATCTGCTCCGTTGTTTTCCAGAAACGATTTGATTATAAAGATCAGAATTTTCTCA CCCTGATGAAAAGATTCAATGAAAACTTCAGGATTCTGAACTCCCCATGGATCCAGGTCTGCAATAATTT CCCTCTACTCATTGATTGTTTCCCAGGAACTCACAACAAAGTGCTTAAAAATGTTGCTCTTACACGAAGT TACATTAGGGAGAAAGTAAAAGAACACCAAGCATCACTGGATGTTAACAATCCTCGGGACTTTATCGATT GCTTCCTGATCAAAATGGAGCAGGAAAAGGACAACCAAAAGTCAGAATTCAATATTGAAAACTTGGTTGG CACTGTAGCTGATCTATTTGTTGCTGGAACAGAGACAACAAGCACCACTCTGAGATATGGACTCCTGCTC CTGCTGAAGCACCCAGAGGTCACAGCTAAAGTCCAGGAAGAGATTGATCATGTAATTGGCAGACACAGGA GCCCCTGCATGCAGGATAGGAGCCACATGCCTTACACTGATGCTGTAGTGCACGAGATCCAGAGATACAG TGACCTTGTCCCCACCGGTGTGCCCCATGCAGTGACCACTGATACTAAGTTCAGAAACTACCTCATCCCC AAGGGCACAACCATAATGGCATTACTGACTTCCGTGCTACATGATGACAAAGAATTTCCTAATCCAAATA TCTTTGACCCTGGCCACTTTCTAGATAAGAATGGCAACTTTAAGAAAAGTGACTACTTCATGCCTTTCTC AGCAGGAAAACGAATTTGTGCAGGAGAAGGACTTGCCCGCATGGAGCTATTTTTATTTCTAACCACAATT TTACAGAACTTTAACCTGAAATCTGTTGATGATTTAAAGAACCTCAATACTACTGCAGTTACCAAAGGGA TTGTTTCTCTGCCACCCTCATACCAGATCTGCTTCATCCCTGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231201 representing NM_001198854
Red=Cloning site Green=Tags(s) MFLQPIAKGIISSNGKRWKEIRRFSLTTLRNFGMGKRSIEDRVQEEAHCLVEELRKTKASPCDPTFILGC APCNVICSVVFQKRFDYKDQNFLTLMKRFNENFRILNSPWIQVCNNFPLLIDCFPGTHNKVLKNVALTRS YIREKVKEHQASLDVNNPRDFIDCFLIKMEQEKDNQKSEFNIENLVGTVADLFVAGTETTSTTLRYGLLL LLKHPEVTAKVQEEIDHVIGRHRSPCMQDRSHMPYTDAVVHEIQRYSDLVPTGVPHAVTTDTKFRNYLIP KGTTIMALLTSVLHDDKEFPNPNIFDPGHFLDKNGNFKKSDYFMPFSAGKRICAGEGLARMELFLFLTTI LQNFNLKSVDDLKNLNTTAVTKGIVSLPPSYQICFIPV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001198854 |
| ORF Size | 1164 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001198854.1, NP_001185783.1 |
| RefSeq Size | 1799 bp |
| RefSeq ORF | 1167 bp |
| Locus ID | 1558 |
| UniProt ID | P10632 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231201 | CYP2C8 (Myc-DDK-tagged)-Human cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), transcript variant 3 |
CNY 3,656.00 |
|
| RC231201L3 | Lenti-ORF clone of CYP2C8 (Myc-DDK-tagged)-Human cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), transcript variant 3 |
CNY 5,890.00 |
|
| RC231201L4 | Lenti-ORF clone of CYP2C8 (mGFP-tagged)-Human cytochrome P450, family 2, subfamily C, polypeptide 8 (CYP2C8), transcript variant 3 |
CNY 5,890.00 |
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