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TJP2 (NM_001170630) Human Tagged ORF Clone
CAT＃: RG230605
- TrueORF®
TJP2 (tGFP-tagged) - Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 6
ORF Plasmid: DDK
"NM_001170630" in other vectors (4)
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CNY 10,830.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C9DUPq21.11; DFNA51; DUP9q21.11; PFIC4; X104; ZO2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230605 representing NM_001170630
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGCGAGGAGACCGCGGGTTTCCACCCCGGCGGGAGCTGTCAGGTTGGCTCCGCGCCCCAGGCA TGGAAGAGCTGATATGGGAACAGTACACTGTGACCCTACAAAAGGATTCCAAAAGAGGATTTGGAATTGC AGTGTCCGGAGGCAGAGACAACCCCCACTTTGAAAATGGAGAAACGTCAATTGTCATTTCTGATGTGCTC CCGGGTGGGCCTGCTGATGGGCTGCTCCAAGAAAATGACAGAGTGGTCATGGTCAATGGCACCCCCATGG AGGATGTGCTTCATTCGTTTGCAGTTCAGCAGCTCAGAAAAAGTGGGAAGGTCGCTGCTATTGTGGTCAA GAGGCCCCGGAAGGTCCAGGTGGCCGCACTTCAGGCCAGCCCTCCCCTGGATCAGGATGACCGGGCTTTT GAGGTGATGGACGAGTTTGATGGCAGAAGTTTCCGGAGTGGCTACAGCGAGAGGAGCCGGCTGAACAGCC ATGGGGGGCGCAGCCGCAGCTGGGAGGACAGCCCGGAAAGGGGGCGTCCCCATGAGCGGGCCCGGAGCCG GGAGCGGGACCTCAGCCGGGACCGGAGCCGTGGCCGGAGCCTGGAGCGGGGCCTGGACCAAGACCATGCG CGCACCCGAGACCGCAGCCGTGGCCGGAGCCTGGAGCGGGGCCTGGACCACGACTTTGGGCCATCCCGGG ACCGGGACCGTGACCGCAGCCGCGGCCGGAGCATTGACCAGGACTACGAGCGAGCCTATCACCGGGCCTA CGACCCAGACTACGAGCGGGCCTACAGCCCGGAGTACAGGCGCGGGGCCCGCCACGATGCCCGCTCTCGG GGACCCCGAAGCCGCAGCCGCGAGCACCCGCACTCACGGAGCCCCAGCCCCGAGCCTAGGGGGCGGCCGG GGCCCATCGGGGTCCTCCTGATGAAAAGCAGAGCGAACGAAGAGTATGGTCTCCGGCTTGGGAGTCAGAT CTTCGTAAAGGAAATGACCCGAACGGGTCTGGCAACTAAAGATGGCAACCTTCACGAAGGAGACATAATT CTCAAGATCAATGGGACTGTAACTGAGAACATGTCTTTAACGGATGCTCGAAAATTGATAGAAAAGTCAA GAGGAAAACTACAGCTAGTGGTGTTGAGAGACAGCCAGCAGACCCTCATCAACATCCCGTCATTAAATGA CAGTGACTCAGAAATAGAAGATATTTCAGAAATAGAGTCAAACCGATCATTTTCTCCAGAGGAGAGACGT CATCAGTATTCTGATTATGATTATCATTCCTCAAGTGAGAAGCTGAAGGAAAGGCCAAGTTCCAGAGAGG ACACGCCGAGCAGATTGTCCAGGATGGGTGCGACACCCACTCCCTTTAAGTCCACAGGGGATATTGCAGG CACAGTTGTCCCAGAGACCAACAAGGAACCCAGATACCAAGAGGACCCCCCAGCTCCTCAACCAAAAGCA GCCCCGAGAACTTTTCTTCGTCCTAGTCCTGAAGATGAAGCAATATATGGCCCTAATACCAAAATGGTAA GGTTCAAGAAGGGAGACAGCGTGGGCCTCCGGTTGGCTGGTGGCAATGATGTCGGGATATTTGTTGCTGG CATTCAAGAAGGGACCTCGGCGGAGCAGGAGGGCCTTCAAGAAGGAGACCAGATTCTGAAGGTGAACACA CAGGATTTCAGAGGATTAGTGCGGGAGGATGCCGTTCTCTACCTGTTAGAAATCCCTAAAGGTGAAATGG TGACCATTTTAGCTCAGAGCCGAGCCGATGTGTATAGAGACATCCTGGCTTGTGGCAGAGGGGATTCGTT TTTTATAAGAAGCCACTTTGAATGTGAGAAGGAAACTCCACAGAGCCTGGCCTTCACCAGAGGGGAGGTC TTCCGAGTGGTAGACACACTGTATGACGGCAAGCTGGGCAACTGGCTGGCTGTGAGGATTGGGAACGAGT TGGAGAAAGGCTTAATCCCCAACAAGAGCAGAGCTGAACAAATGGCCAGTGTTCAAAATGCCCAGAGAGA CAACGCTGGGGACCGGGCAGATTTCTGGAGAATGCGTGGCCAGAGGTCTGGGGTGAAGAAGAACCTGAGG AAAAGTCGGGAAGACCTCACAGCTGTTGTGTCTGTCAGCACCAAGTTCCCAGCTTATGAGAGGGTTTTGC TGCGAGAAGCTGGTTTCAAGAGACCTGTGGTCTTATTCGGCCCCATAGCTGATATAGCAATGGAAAAATT GGCTAATGAGTTACCTGACTGGTTTCAAACTGCTAAAACGGAACCAAAAGATGCAGGATCTGAGAAATCC ACTGGAGTGGTCCGGTTAAATACCGTGAGGCAAATTATTGAACAGGATAAGCATGCACTACTGGATGTGA CTCCGAAAGCTGTGGACCTGTTGAATTACACCCAGTGGTTCCCAATTGTGATTTTTTTCAACCCAGACTC CAGACAAGGTGTCAAAACCATGAGACAAAGGTTAAATCCAACGTCCAACAAAAGTTCTCGAAAGTTATTT GATCAAGCCAACAAGCTTAAAAAAACGTGTGCACACCTTTTTACAGCTACAATCAACCTAAATTCAGCCA ATGATAGCTGGTTTGGCAGCTTAAAGGACACTATTCAGCATCAGCAAGGAGAAGCGGTTTGGGTCTCTGA AGGAAAGATGGAAGGGATGGATGATGACCCCGAAGACCGCATGTCCTACTTAACCGCCATGGGCGCGGAC TATCTGAGTTGCGACAGCCGCCTCATCAGTGACTTTGAAGACACGGACGGTGAAGGAGGCGCCTACACTG ACAATGAGCTGGATGAGCCAGCCGAGGAGCCGCTGGTGTCGTCCATCACCCGCTCCTCGGAGCCGGTGCA GCACGAGGAGGTGAGGCGAGGCAGGCCACGGGCAGGAACAGGAGAGCCTGGTGTTTTCCTTGCACTCTCG TGGACAGCTGTGTGTTCAGGGTGCTGTGGAAGGCATTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230605 representing NM_001170630
Red=Cloning site Green=Tags(s) MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVL PGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAF EVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHA RTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSR GPRSRSREHPHSRSPSPEPRGRPGPIGVLLMKSRANEEYGLRLGSQIFVKEMTRTGLATKDGNLHEGDII LKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSQQTLINIPSLNDSDSEIEDISEIESNRSFSPEERR HQYSDYDYHSSSEKLKERPSSREDTPSRLSRMGATPTPFKSTGDIAGTVVPETNKEPRYQEDPPAPQPKA APRTFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNT QDFRGLVREDAVLYLLEIPKGEMVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEV FRVVDTLYDGKLGNWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRDNAGDRADFWRMRGQRSGVKKNLR KSREDLTAVVSVSTKFPAYERVLLREAGFKRPVVLFGPIADIAMEKLANELPDWFQTAKTEPKDAGSEKS TGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTMRQRLNPTSNKSSRKLF DQANKLKKTCAHLFTATINLNSANDSWFGSLKDTIQHQQGEAVWVSEGKMEGMDDDPEDRMSYLTAMGAD YLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEEVRRGRPRAGTGEPGVFLALS WTAVCSGCCGRHS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001170630 |
| ORF Size | 2979 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001170630.1, NP_001164101.1 |
| RefSeq Size | 4720 bp |
| RefSeq ORF | 2982 bp |
| Locus ID | 9414 |
| Protein Pathways | Tight junction, Vibrio cholerae infection |
| Gene Summary | This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230605 | TJP2 (Myc-DDK-tagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 6 |
CNY 9,880.00 |
|
| RC230605L3 | Lenti-ORF clone of TJP2 (Myc-DDK-tagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 6 |
CNY 11,780.00 |
|
| RC230605L4 | Lenti-ORF clone of TJP2 (mGFP-tagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 6 |
CNY 11,780.00 |
|
| SC329243 | TJP2 (untagged)-Human tight junction protein 2 (zona occludens 2) (TJP2) transcript variant 6 |
CNY 19,100.00 |
