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Semaphorin 3E (SEMA3E) (NM_001178129) Human Tagged ORF Clone
CAT＃: RG230452
- TrueORF®
SEMA3E (tGFP-tagged) - Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 2
ORF Plasmid: DDK
"NM_001178129" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | coll-5; M-SEMAH; M-SemaK; SEMAH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230452 representing NM_001178129
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGGATGAATATCAAGAGAGGCTCTTCGTGGGAGGCAGGGACCTTGTATATTCCCTCAGCTTGG AGAGAATCAGTGACGGCTATAAAGAGATACACTGGCCGAGTACAGCTCTAAAAATGGAAGAATGCATAAT GAAGGGAAAAGATGCGGGTGAATGTGCAAATTATGTTCGGGTTTTGCATCACTATAACAGGACACACCTT CTGACCTGTGGTACTGGAGCTTTTGATCCAGTTTGTGCCTTCATCAGAGTTGGATATCATTTGGAGGATC CTCTGTTTCACCTGGAATCACCCAGATCTGAGAGAGGAAGGGGCAGATGTCCTTTTGACCCCAGCTCCTC CTTCATCTCCACTTTAATTGGTAGTGAATTGTTTGCTGGACTCTACAGTGACTACTGGAGCAGAGACGCT GCGATCTTCCGCAGCATGGGGCGACTGGCCCATATCCGCACTGAGCATGACGATGAGCGTCTGTTGAAAG AACCAAAATTTGTAGGTTCATACATGATTCCTGACAATGAAGACAGAGATGACAACAAAGTATATTTCTT TTTTACTGAGAAGGCACTGGAGGCAGAAAACAATGCTCACGCAATTTACACCAGGGTCGGGCGACTCTGT GTGAATGATGTAGGAGGGCAGAGAATACTGGTGAATAAGTGGAGCACTTTCCTAAAAGCGAGACTCGTTT GCTCAGTACCAGGAATGAATGGAATTGACACATATTTTGATGAATTAGAGGACGTTTTTTTGCTACCTAC CAGAGATCATAAGAATCCAGTGATATTTGGACTCTTTAACACTACCAGTAATATTTTTCGAGGGCATGCT ATATGTGTCTATCACATGTCTAGCATTCGGGCAGCCTTCAACGGACCATATGCACATAAGGAAGGACCTG AATACCACTGGTCAGTCTATGAAGGAAAAGTCCCTTATCCAAGGCCTGGTTCTTGTGCCAGCAAAGTAAA TGGAGGGAGATACGGAACCACCAAGGACTATCCTGATGATGCCATCCGATTTGCAAGAAGTCATCCACTA ATGTACCAGGCCATAAAACCTGCCCATAAAAAACCAATATTGGTAAAAACAGATGGAAAATATAACCTGA AACAAATAGCAGTAGATCGAGTGGAAGCTGAGGATGGCCAATATGACGTCTTGTTTATTGGGACAGATAA TGGAATTGTGCTGAAAGTAATCACAATTTACAACCAAGAAATGGAATCAATGGAAGAAGTAATTCTAGAA GAACTTCAGATATTCAAGGATCCAGTTCCTATTATTTCTATGGAGATTTCTTCAAAGCGGCAACAGCTGT ATATTGGATCTGCTTCTGCTGTGGCTCAAGTCAGATTCCATCACTGTGACATGTATGGAAGTGCTTGTGC TGACTGCTGCCTGGCTCGAGACCCTTACTGTGCCTGGGATGGCATATCCTGCTCCCGGTATTACCCAACA GGCACACATGCAAAAAGGCGTTTCCGGAGACAAGATGTTCGACATGGAAATGCAGCTCAGCAGTGCTTTG GACAACAGTTTGTTGGGGATGCTTTGGATAAGACTGAAGAACATCTGGCTTATGGCATAGAGAACAACAG TACTTTGCTGGAATGTACCCCACGATCTTTACAAGCGAAAGTTATCTGGTTTGTACAGAAAGGACGTGAG ACAAGAAAAGAGGAGGTGAAGACAGATGACAGAGTGGTTAAGATGGACCTTGGTTTACTCTTCCTAAGGT TACACAAATCAGATGCTGGGACCTATTTTTGCCAGACAGTAGAGCATAGCTTTGTCCATACGGTCCGTAA AATCACCTTGGAGGTAGTGGAAGAGGAGAAAGTCGAGGATATGTTTAACAAGGACGATGAGGAGGACAGG CATCACAGGATGCCTTGTCCTGCTCAGAGTAGCATCTCGCAGGGAGCAAAACCATGGTACAAGGAATTCT TGCAGCTGATCGGTTATAGCAACTTCCAGAGAGTGGAAGAATACTGCGAGAAAGTATGGTGCACAGATAG AAAGAGGAAAAAGCTTAAAATGTCACCCTCCAAGTGGAAGTATGCCAACCCTCAGGAAAAGAAGCTCCGT TCCAAACCTGAGCATTACCGCCTGCCCAGGCACACGCTGGACTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230452 representing NM_001178129
Red=Cloning site Green=Tags(s) MLLDEYQERLFVGGRDLVYSLSLERISDGYKEIHWPSTALKMEECIMKGKDAGECANYVRVLHHYNRTHL LTCGTGAFDPVCAFIRVGYHLEDPLFHLESPRSERGRGRCPFDPSSSFISTLIGSELFAGLYSDYWSRDA AIFRSMGRLAHIRTEHDDERLLKEPKFVGSYMIPDNEDRDDNKVYFFFTEKALEAENNAHAIYTRVGRLC VNDVGGQRILVNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLPTRDHKNPVIFGLFNTTSNIFRGHA ICVYHMSSIRAAFNGPYAHKEGPEYHWSVYEGKVPYPRPGSCASKVNGGRYGTTKDYPDDAIRFARSHPL MYQAIKPAHKKPILVKTDGKYNLKQIAVDRVEAEDGQYDVLFIGTDNGIVLKVITIYNQEMESMEEVILE ELQIFKDPVPIISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPT GTHAKRRFRRQDVRHGNAAQQCFGQQFVGDALDKTEEHLAYGIENNSTLLECTPRSLQAKVIWFVQKGRE TRKEEVKTDDRVVKMDLGLLFLRLHKSDAGTYFCQTVEHSFVHTVRKITLEVVEEEKVEDMFNKDDEEDR HHRMPCPAQSSISQGAKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDRKRKKLKMSPSKWKYANPQEKKLR SKPEHYRLPRHTLDS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001178129 |
| ORF Size | 2145 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001178129.1, NP_001171600.1 |
| RefSeq Size | 5993 bp |
| RefSeq ORF | 2148 bp |
| Locus ID | 9723 |
| UniProt ID | O15041 |
| Protein Families | Secreted Protein |
| Protein Pathways | Axon guidance |
| Gene Summary | Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230452 | SEMA3E (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 2 |
CNY 7,130.00 |
|
| RC230452L3 | Lenti-ORF clone of SEMA3E (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 2 |
CNY 9,030.00 |
|
| RC230452L4 | Lenti-ORF clone of SEMA3E (mGFP-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 2 |
CNY 9,030.00 |
|
| SC329090 | SEMA3E (untagged)-Human sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3E (SEMA3E) transcript variant 2 |
CNY 7,220.00 |
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