United States
Germany
Japan
United Kingdom
China
GGA3 (NM_001172703) Human Tagged ORF Clone
CAT＃: RG230394
- TrueORF®
GGA3 (tGFP-tagged) - Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 1
ORF Plasmid: DDK
"NM_001172703" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230394 representing NM_001172703
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAACTGTGGGAGGAGATTTCATAACGAAGTGGGGAAGTTCCGCTTTTTGAATGAGTTAATCAAAG TCGTCTCTCCAAAGTACCTGGGGGACAGGGTGTCTGAGAAAGTGAAGACCAAGGTTATTGAGCTGCTGTA CAGCTGGACCATGGCCCTGCCAGAAGAAGCAAAGATCAAAGACGCCTACCACATGCTGAAGAGACAGGGC ATAGTGCAGTCTGACCCACCAATTCCTGTGGATAGGACGCTGATCCCCTCTCCACCACCTCGTCCCAAAA ACCCTGTTTTTGATGATGAGGAGAAGTCCAAGCTTTTAGCCAAGCTGCTGAAAAGCAAAAACCCAGATGA CCTGCAGGAGGCCAACAAGCTCATCAAGTCCATGGTGAAGGAAGACGAGGCACGGATCCAGAAGGTGACC AAGCGTCTGCACACGTTAGAGGAAGTTAACAACAACGTGAGACTGCTCAGTGAGATGCTGCTTCATTACA GCCAGGAGGACTCTTCGGACGGGGACAGAGAGCTGATGAAGGAGCTGTTTGATCAGTGTGAGAACAAGAG GCGGACTTTATTTAAACTCGCCAGTGAGACTGAGGACAATGATAACAGTTTGGGGGACATCCTGCAAGCC AGTGACAACCTCTCCCGGGTCATCAACTCTTACAAAACAATTATTGAAGGGCAGGTCATCAATGGCGAGG TGGCTACCTTAACCCTGCCTGACTCGGAAGGAAACAGTCAGTGCAGTAACCAAGGCACGCTCATCGACCT TGCGGAGCTGGACACGACCAACAGTTTGTCCTCCGTGTTGGCCCCAGCACCTACTCCACCCTCCTCAGGC ATCCCAATCCTCCCTCCACCACCCCAGGCCTCAGGACCTCCACGGAGCCGCTCCTCTAGCCAGGCCGAGG CCACCCTGGGGCCCAGCAGCACAAGCAACGCCCTCTCCTGGCTGGACGAGGAGCTACTCTGCTTGGGCCT CGCCGACCCAGCCCCTAATGTTCCTCCCAAAGAGTCAGCTGGGAACAGCCAGTGGCACCTGCTCCAGAGG GAACAGTCCGACCTGGACTTCTTCAGCCCCAGGCCGGGGACCGCTGCCTGTGGCGCCTCCGATGCTCCTC TGCTCCAGCCCTCAGCCCCCTCCTCAAGCAGCTCCCAAGCTCCACTGCCGCCTCCCTTCCCAGCTCCTGT GGTCCCAGCCAGTGTTCCTGCCCCCAGTGCGGGCTCCTCCTTGTTTTCTACTGGAGTGGCCCCAGCCTTG GCCCCAAAAGTTGAGCCCGCAGTCCCTGGGCACCATGGCTTGGCGTTGGGCAACAGCGCGCTGCACCACC TGGATGCCCTCGATCAGCTTCTAGAAGAGGCCAAAGTGACCTCGGGCTTGGTGAAACCCACTACCTCCCC TCTCATCCCCACCACCACCCCAGCCAGGCCCCTCCTGCCCTTCTCCACGGGGCCCGGCAGCCCGCTCTTC CAGCCACTGAGTTTCCAGTCCCAGGGCAGCCCCCCGAAGGGGCCTGAGCTCTCCCTGGCCAGCATCCACG TGCCCCTGGAATCGATCAAGCCTAGCAGTGCCCTTCCTGTGACAGCCTACGATAAAAACGGCTTCCGCAT CCTCTTCCACTTTGCCAAGGAGTGTCCCCCAGGACGACCTGACGTGCTGGTGGTGGTGGTGTCCATGCTG AACACGGCTCCCTTACCTGTCAAGAGCATCGTGCTGCAGGCTGCAGTGCCCAAGTCAATGAAAGTGAAGT TGCAGCCACCTTCTGGGACAGAACTCTCTCCATTTAGCCCCATCCAGCCACCTGCAGCCATCACCCAGGT CATGTTGCTGGCCAATCCACTGAAGGAGAAGGTGCGGCTTCGGTATAAGCTGACCTTCGCCCTGGGGGAG CAGCTGAGCACAGAGGTGGGCGAGGTGGACCAGTTCCCTCCTGTGGAACAGTGGGGGAACCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230394 representing NM_001172703
Red=Cloning site Green=Tags(s) MKNCGRRFHNEVGKFRFLNELIKVVSPKYLGDRVSEKVKTKVIELLYSWTMALPEEAKIKDAYHMLKRQG IVQSDPPIPVDRTLIPSPPPRPKNPVFDDEEKSKLLAKLLKSKNPDDLQEANKLIKSMVKEDEARIQKVT KRLHTLEEVNNNVRLLSEMLLHYSQEDSSDGDRELMKELFDQCENKRRTLFKLASETEDNDNSLGDILQA SDNLSRVINSYKTIIEGQVINGEVATLTLPDSEGNSQCSNQGTLIDLAELDTTNSLSSVLAPAPTPPSSG IPILPPPPQASGPPRSRSSSQAEATLGPSSTSNALSWLDEELLCLGLADPAPNVPPKESAGNSQWHLLQR EQSDLDFFSPRPGTAACGASDAPLLQPSAPSSSSSQAPLPPPFPAPVVPASVPAPSAGSSLFSTGVAPAL APKVEPAVPGHHGLALGNSALHHLDALDQLLEEAKVTSGLVKPTTSPLIPTTTPARPLLPFSTGPGSPLF QPLSFQSQGSPPKGPELSLASIHVPLESIKPSSALPVTAYDKNGFRILFHFAKECPPGRPDVLVVVVSML NTAPLPVKSIVLQAAVPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLLANPLKEKVRLRYKLTFALGE QLSTEVGEVDQFPPVEQWGNL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001172703 |
| ORF Size | 1953 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001172703.3 |
| RefSeq Size | 3921 bp |
| RefSeq ORF | 1956 bp |
| Locus ID | 23163 |
| UniProt ID | Q9NZ52 |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome |
| Gene Summary | This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230394 | GGA3 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 1 |
CNY 4,560.00 |
|
| RC230394L3 | Lenti-ORF clone of GGA3 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 1 |
CNY 6,460.00 |
|
| RC230394L4 | Lenti-ORF clone of GGA3 (mGFP-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 1 |
CNY 6,460.00 |
|
| SC329032 | GGA3 (untagged)-Human golgi-associated gamma adaptin ear containing ARF binding protein 3 (GGA3) transcript variant 1 |
CNY 10,450.00 |
