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FUS (NM_001170937) Human Tagged ORF Clone
CAT＃: RG230222
- TrueORF®
FUS (tGFP-tagged) - Human fused in sarcoma (FUS), transcript variant 4. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001170937" in other vectors (4)
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CNY 4,940.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ALS6; altFUS; ETM4; FUS1; HNRNPP2; POMP75; TLS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230222 ORF sequence, codon optimized.
Due to the complexity of NM_001170937, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCAATGACTATACGCAGCAGGCCACCCAATCCTACGGCGCCTACCCAACTCAGCCCGGTCAGG GTTACTCCCAGCAGTCCTCCCAACCATACGGACAGCAGAGCTATAGTGGATATAGCCAAAGCACGGATAC ATCTGGGTATGGCCAGTCATCATACAGCAGTTACGGGCAATCACAGAACACCGGCTACGGAACGCAGAGT ACCCCACAGGGTTACGGATCTACAGGCGGCTATGGCTCTAGCCAGTCATCCCAAAGTAGCTATGGGCAGC AGAGCTCTTACCCGGGGTACGGGCAGCAGCCTGCCCCGAGTTCAACCAGCGGATCCTACGGCTCTTCCAG CCAGTCTTCTAGCTACGGCCAGCCCCAGTCCGGCTCCTACTCTCAGCAGCCTTCCTATGGCGGTCAGCAG CAGTCATACGGCCAGCAGCAAAGTTATAACCCACCACAAGGTTACGGACAGCAGAACCAGTATAATTCTT CCTCAGGGGGAGGGGGCGGCGGGAATTACGGACAGGACCAGAGCTCCATGTCTAGCGGGGGGGGCTCTGG CGGCGGATATGGCAATCAGGATCAGTCCGGGGGCGGAGGGAGCGGTGGATATGGCCAGCAGGACAGGGGC GGCCGCGGGAGAGGCGGGAGCGGAGGAGGGGGAGGCGGAGGGGGCGGGGGATACAACCGGAGCAGTGGCG GTTATGAGCCTCGCGGTCGAGGGGGAGGGAGAGGGGGCAGAGGCGGTATGGGTGGAAGCGACCGCGGGGG TTTTAACAAGTTTGGAGGGCCACGGGACCAAGGCAGCAGACATGATTCCGAGCAAGATAACTCCGACAAC AACACCATTTTCGTCCAAGGCCTTGGAGAGAACGTCACAATCGAAAGCGTGGCCGATTACTTTAAGCAGA TTGGAATAATCAAAACAAACAAAAAGACCGGCCAACCTATGATTAACTTGTACACCGACCGCGAAACAGG CAAGCTTAAGGGAGAGGCAACCGTCAGTTTTGATGATCCACCCAGCGCCAAAGCGGCCATTGATTGGTTC GATGGCAAGGAGTTCTCTGGCAATCCCATTAAGGTAAGCTTTGCAACGAGGCGCGCTGATTTCAATAGAG GCGGGGGAAATGGTCGCGGAGGGCGCGGACGAGGAGGACCAATGGGGCGGGGGGGTTACGGCGGGGGGGG GAGCGGTGGCGGTGGGAGGGGGGGGTTCCCTAGCGGGGGAGGGGGTGGTGGGGGTCAGCAGAGGGCTGGG GACTGGAAATGTCCCAACCCCACTTGTGAGAATATGAACTTCAGCTGGCGAAATGAATGCAACCAGTGCA AGGCGCCGAAACCTGATGGACCTGGTGGGGGACCAGGGGGCTCACACATGGGCGGCAATTACGGGGATGA CCGAAGGGGCGGGCGCGGCGGCTATGATAGAGGCGGCTACCGCGGGCGGGGAGGTGACAGGGGTGGGTTC CGCGGGGGGAGGGGTGGCGGTGATAGGGGAGGCTTCGGTCCTGGCAAGATGGATTCCAGAGGCGAACACA GGCAGGACAGGAGGGAGAGGCCCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230222 representing NM_001170937
Red=Cloning site Green=Tags(s) MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQSQNTGYGTQS TPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQ QSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRG GRGRGGSGGGGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNSDN NTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWF DGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQQRAG DWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGF RGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001170937 |
| ORF Size | 1566 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001170937.1, NP_001164408.1 |
| RefSeq Size | 5107 bp |
| RefSeq ORF | 1569 bp |
| Locus ID | 2521 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Gene Summary | This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230222 | FUS (Myc-DDK-tagged)-Human fused in sarcoma (FUS), transcript variant 4. Note: ORF is codon optimized |
CNY 3,888.00 |
|
| RC230222L3 | Lenti-ORF clone of FUS (Myc-DDK-tagged)-Human fused in sarcoma (FUS), transcript variant 4. Note: ORF is codon optimized |
CNY 6,370.00 |
|
| RC230222L4 | Lenti-ORF clone of FUS (mGFP-tagged)-Human fused in sarcoma (FUS), transcript variant 4. Note: ORF is codon optimized |
CNY 6,370.00 |
|
| SC328860 | FUS (untagged)-Human fused in sarcoma (FUS) transcript variant 4 |
CNY 8,360.00 |
