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Cripto1 (TDGF1) (NM_001174136) Human Tagged ORF Clone
CAT＃: RG229631
- TrueORF®
TDGF1 (tGFP-tagged) - Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 2
ORF Plasmid: DDK
"NM_001174136" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CR; CR-1; CRGF; CRIPTO |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229631 representing NM_001174136
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATTTCTAAAGTCTTTGAACTGGGATTAGTTGCCGGGCTGGGCCATCAGGAATTTGCTCGTCCAT CTCGGGGATACCTGGCCTTCAGAGATGACAGCATTTGGCCCCAGGAGGAGCCTGCAATTCGGCCTCGGTC TTCCCAGCGTGTGCCGCCCATGGGGATACAGCACAGTAAGGAGCTAAACAGAACCTGCTGCCTGAATGGG GGAACCTGCATGCTGGGGTCCTTTTGTGCCTGCCCTCCCTCCTTCTACGGACGGAACTGTGAGCACGATG TGCGCAAAGAGAACTGTGGGTCTGTGCCCCATGACACCTGGCTGCCCAAGAAGTGTTCCCTGTGTAAATG CTGGCACGGTCAGCTCCGCTGCTTTCCTCAGGCATTTCTACCCGGCTGTGATGGCCTTGTGATGGATGAG CACCTCGTGGCTTCCAGGACTCCAGAACTACCACCGTCTGCACGTACTACCACTTTTATGCTAGTTGGCA TCTGCCTTTCTATACAAAGCTACTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229631 representing NM_001174136
Red=Cloning site Green=Tags(s) MAISKVFELGLVAGLGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPPMGIQHSKELNRTCCLNG GTCMLGSFCACPPSFYGRNCEHDVRKENCGSVPHDTWLPKKCSLCKCWHGQLRCFPQAFLPGCDGLVMDE HLVASRTPELPPSARTTTFMLVGICLSIQSYY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001174136 |
| ORF Size | 516 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001174136.2 |
| RefSeq Size | 1784 bp |
| RefSeq ORF | 519 bp |
| Locus ID | 6997 |
| UniProt ID | P13385 |
| Protein Families | Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane |
| Gene Summary | This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229631 | TDGF1 (Myc-DDK-tagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 2 |
CNY 3,990.00 |
|
| RC229631L1 | Lenti-ORF clone of TDGF1 (Myc-DDK-tagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 2 |
CNY 5,040.00 |
|
| RC229631L2 | Lenti-ORF clone of TDGF1 (mGFP-tagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 2 |
CNY 5,890.00 |
|
| RC229631L3 | Lenti-ORF clone of TDGF1 (Myc-DDK-tagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 2 |
CNY 5,890.00 |
|
| RC229631L4 | Lenti-ORF clone of TDGF1 (mGFP-tagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1), transcript variant 2 |
CNY 5,890.00 |
|
| SC328269 | TDGF1 (untagged)-Human teratocarcinoma-derived growth factor 1 (TDGF1) transcript variant 2 |
CNY 3,990.00 |
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