United States
Germany
Japan
United Kingdom
China
COASY (NM_001042532) Human Tagged ORF Clone
CAT＃: RG229299
- TrueORF®
COASY (tGFP-tagged) - Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4
ORF Plasmid: DDK
"NM_001042532" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DPCK; NBIA6; NBP; PCH12; pOV-2; PPAT; UKR1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229299 representing NM_001042532
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACACCAAGGCTTAGAGCACAGCCCCGAGGCGCCGTCTACCAGGCCCCGTCCCCTCCCCCGGCTC CTGTCGGCCTGGGCAGCATGGCCGTATTCCGGTCGGGTCTCCTGGTGCTGACGACGCCGCTGGCCTCCCT AGCCCCTCGCCTGGCCTCCATCCTGACCTCGGCGGCCCGGCTGGTGAATCACACACTCTATGTTCACCTG CAGCCGGGCATGAGCCTGGAGGGCCCGGCTCAGCCCCAGTCCAGCCCCGTGCAGGCCACGTTTGAGGTTC TTGATTTCATCACGCACCTCTATGCTGGCGCCGACGTCCACAGGCACTTGGACGTCAGAATCCTACTGAC CAATATCCGAACCAAGAGCACCTTTCTCCCTCCCCTGCCCACCTCAGTCCAGAATCTCGCCCACCCGCCA GAAGTCGTGTTGACAGATTTCCAGACCCTGGATGGAAGCCAGTACAACCCGGTCAAACAGCAGCTAGTGC GTTACGCCACCAGCTGTTACAGCTGTTGTCCGCGACTGGCCTCGGTGCTGCTATACTCCGATTATGGGAT AGGAGAAGTGCCCGTGGAGCCCCTGGATGTCCCCTTACCCTCCACGATCAGGCCAGCTTCCCCCGTGGCC GGGTCTCCAAAGCAGCCGGTGCGTGGCTACTACCGTGGCGCTGTCGGTGGCACGTTTGACCGCCTGCACA ACGCCCACAAGGTGTTGCTCAGTGTCGCGTGCATCCTGGCCCAGGAGCAGCTTGTGGTGGGAGTAGCAGA CAAAGATCTGTTGAAGAGCAAGTTGCTCCCTGAGCTGCTCCAACCTTATACAGAACGTGTGGAACATCTG AGTGAATTCCTGGTGGACATCAAGCCCTCCTTGACTTTTGATGTCATCCCCCTGCTGGACCCCTATGGGC CCGCTGGCTCTGACCCCTCCCTGGAGTTCCTGGTGGTCAGCGAGGAGACCTATCGTGGGGGGATGGCCAT CAACCGCTTCCGCCTTGAGAATGACCTGGAGGAACTTGCTTTGTACCAGATCCAGCTGCTGAAGGACCTC AGACATACAGAGAATGAAGAGGACAAAGTCAGCTCCTCCAGCTTCCGCCAGCGAATGTTGGGGAACCTGC TTCGGCCTCCATATGAAAGGCCAGAGCTCCCCACATGTCTCTATGTAATTGGGCTGACTGGCATCAGTGG CTCTGGGAAGAGCTCAATAGCTCAGCGACTGAAGGGCCTGGGGGCGTTTGTCATTGACAGTGACCACCTG GGTCATCGGGCCTATGCCCCAGGTGGCCCTGCCTACCAGCCTGTGGTGGAGGCCTTTGGAACAGATATTC TCCATAAAGATGGCATCATCAACAGGAAGGTCCTAGGCAGCCGGGTGTTTGGGAATAAGAAGCAGCTGAA GATACTCACGGACATTATGTGGCCAATTATCGCAAAGCTGGCCCGAGAGGAGATGGATCGGGCTGTGGCT GAGGGAAAGCGTGTGTGTGTGATTGATGCCGCTGTGTTGCTTGAAGCCGGCTGGCAGAACCTGGTCCATG AGGTGTGGACTGCTGTCATCCCAGAGACTGAGGCTGTAAGACGCATTGTGGAGAGGGATGGCCTCAGTGA AGCCGCGGCTCAAAGCCGGCTGCAGAGCCAGATGAGCGGGCAGCAGCTTGTGGAACAGAGCCACGTGGTG CTCAGCACCTTGTGGGAGCCGCATATCACCCAACGCCAGGTGGAGAAAGCCTGGGCCCTCTTGCAGAAGC GCATTCCCAAGACTCATCAGGCCCTCGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229299 representing NM_001042532
Red=Cloning site Green=Tags(s) MRTPRLRAQPRGAVYQAPSPPPAPVGLGSMAVFRSGLLVLTTPLASLAPRLASILTSAARLVNHTLYVHL QPGMSLEGPAQPQSSPVQATFEVLDFITHLYAGADVHRHLDVRILLTNIRTKSTFLPPLPTSVQNLAHPP EVVLTDFQTLDGSQYNPVKQQLVRYATSCYSCCPRLASVLLYSDYGIGEVPVEPLDVPLPSTIRPASPVA GSPKQPVRGYYRGAVGGTFDRLHNAHKVLLSVACILAQEQLVVGVADKDLLKSKLLPELLQPYTERVEHL SEFLVDIKPSLTFDVIPLLDPYGPAGSDPSLEFLVVSEETYRGGMAINRFRLENDLEELALYQIQLLKDL RHTENEEDKVSSSSFRQRMLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHL GHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVA EGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVV LSTLWEPHITQRQVEKAWALLQKRIPKTHQALD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042532 |
| ORF Size | 1779 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042532.4 |
| RefSeq Size | 2331 bp |
| RefSeq ORF | 1782 bp |
| Locus ID | 80347 |
| UniProt ID | Q13057 |
| Protein Pathways | Metabolic pathways, Pantothenate and CoA biosynthesis |
| Gene Summary | Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms. [provided by RefSeq, Apr 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229299 | COASY (Myc-DDK-tagged)-Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4 |
CNY 4,416.00 |
|
| RC229299L3 | Lenti-ORF clone of COASY (Myc-DDK-tagged)-Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4 |
CNY 6,460.00 |
|
| RC229299L4 | Lenti-ORF clone of COASY (mGFP-tagged)-Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4 |
CNY 6,460.00 |
|
| SC311397 | COASY (untagged)-Human CoA synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 4 |
CNY 9,600.00 |
|
| SC327934 | COASY (untagged)-Human Coenzyme A synthase (COASY) nuclear gene encoding mitochondrial protein transcript variant 4 |
CNY 9,600.00 |
