United States
Germany
Japan
United Kingdom
China
HSPC302 (TBCK) (NM_001163437) Human Tagged ORF Clone
CAT＃: RG229029
- TrueORF®
TBCK (tGFP-tagged) - Human TBC1 domain containing kinase (TBCK), transcript variant 3
ORF Plasmid: DDK
"NM_001163437" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,410.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HSPC302; IHPRF3; TBCKL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229029 representing NM_001163437
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCCCCTGAAGGACGCTGAAATGGGAGCCTTTACCTTCTTTGCCTCGGCTCTGCCACATGATGTTT GTGGAAGCAATGGACTTCCTCTCACACCAAATTCCATCAAAATTTTAGGGCGCTTTCAAATCCTTAAAAC CATCACCCATCCCAGACTCTGCCAGTATGTGGATATTTCTAGGGGAAAGCATGAACGACTAGTGGTCGTG GCTGAACATTGTGAACGTAGTCTGGAAGACTTGCTTCGAGAAAGGAAACCTGTGAGCTGTTCAACGGTTT TGTGTATAGCATTTGAGGTTCTTCAGGGCTTGCAGTATATGAACAAACATGGTATAGTACACAGGGCATT GTCTCCTCATAATATCCTGTTGGACCGAAAGGGACATATTAAATTGGCTAAATTTGGACTTTATCACATG ACAGCTCATGGTGATGATGTTGATTTCCCAATAGGGTATCCCTCGTACTTGGCCCCTGAGGGAAGAAAAT TATTTCAGAGCTTGGATATTTCTGAAAGACTAAAATTTTTGCTTACTTTGGATTGTGTAGATGACACTTT AATAGTTCTGGCTGAAGAGCATGGTTGTTTGGACATTATAAAGGAGCTTCCTGAAACTGTGATAGATCTT TTGAATAAGTGCCTTACCTTCCATCCTTCTAAGAGGCCAACCCCAGATGAATTAATGAAGGACAAAGTAT TCAGTGAGGTATCACCTTTATATACCCCCTTTACCAAACCTGCCAGTCTGTTTTCATCTTCTCTGAGATG TGCTGATTTAACTCTGCCTGAGGATATCAGTCAGTTGTGTAAAGATATAAATAATGATTACCTGGCAGAA AGATCTATTGAAGAAGTGTATTACCTTTGGTGTTTGGCTGGAGGTGACTTGGAGAAAGAGCTTGTCAACA AGGAAATCATTCGATCCAAACCACCTATCTGCACACTCCCCAATTTTCTCTTTGAGGATGGTGAAAGCTT TGGACAAGGTCGAGATAGAAGCTCGCTTTTAGATGATACCACTGTGACATTGTCGTTATGCCAGCTAAGA AATAGATTGAAAGATGTTGGTGGAGAAGCATTTTACCCATTACTTGAAGATGACCAGTCTAATTTACCTC ATTCAAACAGCAATAATGAGTTGTCTGCAGCTGCCACGCTCCCTTTAATCATCAGAGAGAAGGATACAGA GTACCAACTAAATAGAATTATTCTCTTCGACAGGCTGCTAAAGGCTTATCCATATAAAAAAAACCAAATC TGGAAAGAAGCAAGAGTTGACATTCCTCCTCTTATGAGAGGTTTAACCTGGGCTGCTCTTCTGGGAGTTG AGGGAGCTATTCATGCCAAGTACGATGCAATTGATAAAGACACTCCAATTCCTACAGATAGACAAATTGA AGTGGATATTCCTCGCTGTCATCAGTACGATGAACTGTTATCATCACCAGAAGGTCATGCAAAATTTAGG CGTGTATTAAAAGCCTGGGTAGTGTCTCATCCTGATCTTGTGTATTGGCAAGGTCTTGACTCACTTTGTG CTCCATTCCTATATCTAAACTTCAATAATGAAGCCTTGGCTTATGCATGTATGTCTGCTTTTATTCCCAA ATACCTGTATAACTTCTTCTTAAAAGACAACTCACATGTAATACAAGAGTATCTGACTGTCTTCTCTCAG ATGATTGCATTTCATGATCCAGAGCTGAGTAATCATCTCAATGAGATTGGTTTCATTCCAGATCTCTATG CCATCCCTTGGTTTCTTACCATGTTTACTCATGTATTTCCACTACACAAAATTTTCCACCTCTGGGATAC CTTACTACTTGGGAATTCCTCTTTCCCATTCTGTATTGGAGTAGCAATTCTTCAGCAGCTGCGGGACCGG CTTTTGGCTAATGGCTTTAATGAGTGTATTCTTCTCTTCTCCGATTTACCAGAAATTGACATTGAACGCT GTGTGAGAGAATCTATCAACCTGTTTTGTTGGACTCCTAAAAGTGCTACTTACAGACAGCATGCTCAACC TCCAAAGCCATCTTCTGACAGCAGTGGAGGCAGAAGTTCGGCACCTTATTTCTCTGCTGAGTGTCCAGAT CCTCCAAAGACAGATCTGTCAAGAGAATCCATCCCATTAAATGACCTGAAGTCAGAAGTATCACCACGGA TTTCAGCAGAGGACCTGATTGACTTGTGTGAGCTCACAGTGACAGGCCACTTCAAAACACCCAGCAAGAA AACAAAGTCCAGTAAACCAAAGCTCCTGGTGGTTGACATCCGGAATAGTGAAGACTTTATTCGTGGTCAC ATTTCAGGAAGCATCAACATTCCATTCAGTGCTGCCTTCACTGCAGAAGGGGAGCTTACCCAGGGCCCTT ACACTGCTATGCTCCAGAACTTCAAAGGGAAGGTCATTGTCATCGTGGGGCATGTGGCAAAACACACAGC TGAGTTTGCAGCTCACCTTGTGAAGATGAAATATCCAAGAATCTGTATTCTAGATGGTGGCATTAATAAA ATAAAGCCAACAGGCCTCCTCACCATCCCATCTCCTCAAATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229029 representing NM_001163437
Red=Cloning site Green=Tags(s) MFPLKDAEMGAFTFFASALPHDVCGSNGLPLTPNSIKILGRFQILKTITHPRLCQYVDISRGKHERLVVV AEHCERSLEDLLRERKPVSCSTVLCIAFEVLQGLQYMNKHGIVHRALSPHNILLDRKGHIKLAKFGLYHM TAHGDDVDFPIGYPSYLAPEGRKLFQSLDISERLKFLLTLDCVDDTLIVLAEEHGCLDIIKELPETVIDL LNKCLTFHPSKRPTPDELMKDKVFSEVSPLYTPFTKPASLFSSSLRCADLTLPEDISQLCKDINNDYLAE RSIEEVYYLWCLAGGDLEKELVNKEIIRSKPPICTLPNFLFEDGESFGQGRDRSSLLDDTTVTLSLCQLR NRLKDVGGEAFYPLLEDDQSNLPHSNSNNELSAAATLPLIIREKDTEYQLNRIILFDRLLKAYPYKKNQI WKEARVDIPPLMRGLTWAALLGVEGAIHAKYDAIDKDTPIPTDRQIEVDIPRCHQYDELLSSPEGHAKFR RVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLTVFSQ MIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAILQQLRDR LLANGFNECILLFSDLPEIDIERCVRESINLFCWTPKSATYRQHAQPPKPSSDSSGGRSSAPYFSAECPD PPKTDLSRESIPLNDLKSEVSPRISAEDLIDLCELTVTGHFKTPSKKTKSSKPKLLVVDIRNSEDFIRGH ISGSINIPFSAAFTAEGELTQGPYTAMLQNFKGKVIVIVGHVAKHTAEFAAHLVKMKYPRICILDGGINK IKPTGLLTIPSPQI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001163437 |
| ORF Size | 2562 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001163437.2 |
| RefSeq Size | 3424 bp |
| RefSeq ORF | 2565 bp |
| Locus ID | 93627 |
| UniProt ID | Q8TEA7 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a protein that contains a protein kinase domain, a Rhodanase-like domain and the Tre-2/Bub2/Cdc16 (TBC) domain. The encoded protein is thought to play a role in actin organization, cell growth and cell proliferation by regulating the mammalian target of the rapamycin (mTOR) signaling pathway. This protein may also be involved in the transcriptional regulation of the components of the mTOR complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229029 | TBCK (Myc-DDK-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 3 |
CNY 8,550.00 |
|
| RC229029L3 | Lenti-ORF clone of TBCK (Myc-DDK-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 3 |
CNY 10,450.00 |
|
| RC229029L4 | Lenti-ORF clone of TBCK (mGFP-tagged)-Human TBC1 domain containing kinase (TBCK), transcript variant 3 |
CNY 10,450.00 |
|
| SC327664 | TBCK (untagged)-Human TBC1 domain containing kinase (TBCK) transcript variant 3 |
CNY 7,220.00 |
其它HSPC302产品
