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DBC2 (RHOBTB2) (NM_001160036) Human Tagged ORF Clone
CAT＃: RG229009
- TrueORF®
RHOBTB2 (tGFP-tagged) - Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1
ORF Plasmid: DDK
"NM_001160036" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DBC2; DEE64; EIEE64; p83 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229009 representing NM_001160036
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCTGACATGGATTATGAAAGGCCAAACGTAGAGACCATCAAGTGCGTTGTGGTGGGGGACAACG CCGTGGGTAAGACCAGGCTCATCTGTGCCCGCGCTTGCAATGCCACCCTCACCCAGTACCAGCTGCTGGC CACGCATGTGCCCACAGTATGGGCCATCGACCAATATCGTGTGTGCCAGGAGGTGCTGGAACGCTCCCGA GACGTGGTAGATGATGTCAGCGTCTCTCTGCGCCTCTGGGACACCTTTGGAGACCACCACAAAGACCGTC GCTTTGCTTATGGGAGATCTGATGTGGTGGTTCTGTGCTTCTCCATTGCCAACCCCAATTCCCTCCACCA TGTCAAGACCATGTGGTACCCAGAAATCAAGCACTTCTGCCCCCGAGCACCTGTCATCTTGGTGGGCTGC CAGTTGGACCTGCGCTACGCTGACCTGGAGGCTGTCAACAGGGCTAGGCGACCCTTGGCTAGGCCCATCA AACCTAATGAAATCCTGCCCCCAGAGAAGGGTCGGGAGGTGGCCAAGGAGCTGGGCATCCCCTACTATGA GACCAGCGTGGTGGCCCAGTTCGGCATCAAGGACGTCTTTGACAACGCCATCCGAGCTGCACTCATCTCC CGCCGCCACCTGCAGTTCTGGAAGTCCCACCTCCGCAATGTGCAGCGGCCTCTGCTGCAGGCACCCTTCC TACCCCCCAAGCCACCGCCCCCGATCATCGTGGTGCCCGACCCTCCCTCCAGCAGCGAGGAGTGCCCCGC CCACCTCCTGGAGGACCCGCTCTGCGCGGACGTCATCCTGGTGCTGCAGGAGCGGGTGCGCATCTTTGCC CACAAGATCTACCTCTCCACCTCCTCCTCCAAGTTCTATGACCTGTTCCTCATGGACCTGAGTGAGGGGG AGCTGGGGGGCCCCTCGGAGCCAGGGGGCACCCACCCAGAGGACCACCAGGGCCACTCTGATCAACACCA CCACCATCACCACCACCACCATGGGCGAGACTTCCTGCTCCGAGCAGCCAGCTTTGACGTGTGCGAGAGC GTGGATGAGGCTGGGGGCTCCGGTCCTGCTGGCCTCCGTGCTTCCACCAGCGACGGGATCTTACGGGGCA ACGGAACAGGGTACCTACCGGGCAGGGGTCGTGTGCTGTCTTCCTGGAGCCGAGCTTTTGTGAGCATCCA GGAAGAGATGGCAGAAGATCCTCTCACCTACAAATCCCGGCTGATGGTGGTGGTGAAGATGGACAGTTCC ATCCAGCCGGGGCCCTTCCGGGCTGTCCTCAAGTACCTGTACACGGGGGAGCTAGATGAGAACGAGCGTG ACCTCATGCACATTGCCCACATTGCTGAGCTGCTCGAGGTCTTTGATCTGCGCATGATGGTGGCCAACAT TCTCAACAATGAGGCCTTCATGAACCAGGAGATCACCAAGGCCTTCCACGTCCGCCGGACCAACCGGGTT AAGGAGTGCTTGGCAAAAGGCACCTTCTCAGATGTGACCTTCATCCTGGATGATGGCACCATCAGCGCCC ACAAGCCCCTGTTGATTTCCAGCTGTGACTGGATGGCTGCCATGTTTGGGGGGCCATTTGTGGAGAGCTC CACCCGGGAGGTGGTGTTTCCCTACACAAGCAAGAGCTGCATGCGGGCCGTGCTGGAATACCTCTACACC GGCATGTTCACCTCCAGCCCCGACCTGGATGACATGAAGCTCATCATTCTAGCCAACCGCCTCTGCCTGC CACACCTGGTTGCCCTCACAGAGCAGTACACAGTGACCGGGCTGATGGAAGCGACCCAGATGATGGTGGA CATCGATGGGGACGTCCTTGTGTTCCTGGAACTGGCTCAGTTCCACTGTGCGTACCAGCTGGCCGACTGG TGTCTCCACCACATCTGCACCAACTACAACAACGTGTGCCGCAAGTTCCCCCGAGACATGAAGGCCATGT CCCCAGAAAACCAGGAGTATTTCGAGAAGCATCGGTGGCCACCTGTGTGGTACCTGAAGGAGGAAGATCA TTACCAGCGGGCACGGAAGGAGCGTGAGAAGGAGGACTACCTCCACCTCAAGCGGCAGCCCAAACGGCGT TGGCTCTTCTGGAACAGTCCATCCTCCCCGTCTTCCTCGGCAGCCTCCTCCTCATCCCCATCTTCCTCCT CGGCTGTGGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229009 representing NM_001160036
Red=Cloning site Green=Tags(s) MDSDMDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSR DVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLHHVKTMWYPEIKHFCPRAPVILVGC QLDLRYADLEAVNRARRPLARPIKPNEILPPEKGREVAKELGIPYYETSVVAQFGIKDVFDNAIRAALIS RRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIIVVPDPPSSSEECPAHLLEDPLCADVILVLQERVRIFA HKIYLSTSSSKFYDLFLMDLSEGELGGPSEPGGTHPEDHQGHSDQHHHHHHHHHGRDFLLRAASFDVCES VDEAGGSGPAGLRASTSDGILRGNGTGYLPGRGRVLSSWSRAFVSIQEEMAEDPLTYKSRLMVVVKMDSS IQPGPFRAVLKYLYTGELDENERDLMHIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRV KECLAKGTFSDVTFILDDGTISAHKPLLISSCDWMAAMFGGPFVESSTREVVFPYTSKSCMRAVLEYLYT GMFTSSPDLDDMKLIILANRLCLPHLVALTEQYTVTGLMEATQMMVDIDGDVLVFLELAQFHCAYQLADW CLHHICTNYNNVCRKFPRDMKAMSPENQEYFEKHRWPPVWYLKEEDHYQRARKEREKEDYLHLKRQPKRR WLFWNSPSSPSSSAASSSSPSSSSAVV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001160036 |
| ORF Size | 2247 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001160036.1, NP_001153508.1 |
| RefSeq Size | 5261 bp |
| RefSeq ORF | 2250 bp |
| Locus ID | 23221 |
| UniProt ID | Q9BYZ6 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229009 | RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 8,776.00 |
|
| RC229009L1 | Lenti-ORF clone of RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 11,176.00 |
|
| RC229009L2 | Lenti-ORF clone of RHOBTB2 (mGFP-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 5,890.00 |
|
| RC229009L3 | Lenti-ORF clone of RHOBTB2 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 5,890.00 |
|
| RC229009L4 | Lenti-ORF clone of RHOBTB2 (mGFP-tagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 1 |
CNY 5,890.00 |
|
| SC327644 | RHOBTB2 (untagged)-Human Rho-related BTB domain containing 2 (RHOBTB2) transcript variant 1 |
CNY 7,220.00 |
