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Versican (VCAN) (NM_001164098) Human Tagged ORF Clone
CAT＃: RG228691
- TrueORF®
VCAN (tGFP-tagged) - Human versican (VCAN), transcript variant 4
ORF Plasmid: DDK
"NM_001164098" in other vectors (4)
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CNY 17,960.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CSPG2; ERVR; GHAP; PG-M; WGN; WGN1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG228691 representing NM_001164098
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCATAAATATAAAGAGCATCTTATGGATGTGTTCAACCTTAATAGTAACCCATGCGCTACATAAAG TCAAAGTGGGAAAAAGCCCACCGGTGAGGGGCTCCCTCTCTGGAAAAGTCAGCCTACCTTGTCATTTTTC AACGATGCCTACTTTGCCACCCAGTTACAACACCAGTGAATTTCTCCGCATCAAATGGTCTAAGATTGAA GTGGACAAAAATGGAAAAGATTTGAAAGAGACTACTGTCCTTGTGGCCCAAAATGGAAATATCAAGATTG GTCAGGACTACAAAGGGAGAGTGTCTGTGCCCACACATCCCGAGGCTGTGGGCGATGCCTCCCTCACTGT GGTCAAGCTGCTGGCAAGTGATGCGGGTCTTTACCGCTGTGACGTCATGTACGGGATTGAAGACACACAA GACACGGTGTCACTGACTGTGGATGGGGTTGTGTTTCACTACAGGGCGGCAACCAGCAGGTACACACTGA ATTTTGAGGCTGCTCAGAAGGCTTGTTTGGACGTTGGGGCAGTCATAGCAACTCCAGAGCAGCTCTTTGC TGCCTATGAAGATGGATTTGAGCAGTGTGACGCAGGCTGGCTGGCTGATCAGACTGTCAGATATCCCATC CGGGCTCCCAGAGTAGGCTGTTATGGAGATAAGATGGGAAAGGCAGGAGTCAGGACTTATGGATTCCGTT CTCCCCAGGAAACTTACGATGTGTATTGTTATGTGGATCATCTGGATGGTGATGTGTTCCACCTCACTGT CCCCAGTAAATTCACCTTCGAGGAGGCTGCAAAAGAGTGTGAAAACCAGGATGCCAGGCTGGCAACAGTG GGGGAACTCCAGGCGGCATGGAGGAACGGCTTTGACCAGTGCGATTACGGGTGGCTGTCGGATGCCAGCG TGCGCCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGTGGTCTACTTGGGGTGAGAACCCTGTATCG TTTTGAGAACCAGACAGGCTTCCCTCCCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACCTAAAGAG GCTACAACCATCGATTTGAGTATCCTCGCAGAAACTGCATCACCCAGTTTATCCAAAGAACCACAAATGG TTTCTGATAGAACTACACCAATCATCCCTTTAGTTGATGAATTACCTGTCATTCCAACAGAGTTCCCTCC CGTGGGAAATATTGTCAGTTTTGAACAGAAAGCCACAGTCCAACCTCAGGCTATCACAGATAGTTTAGCC ACCAAATTACCCACACCTACTGGCAGTACCAAGAAGCCCTGGGATATGGATGACTACTCACCTTCTGCTT CAGGACCTCTTGGAAAGCTAGACATATCAGAAATTAAGGAAGAAGTGCTCCAGAGTACAACTGGCGTCTC TCATTATGCTACGGATTCATGGGATGGTGTCGTGGAAGATAAACAAACACAAGAATCGGTTACACAGATT GAACAAATAGAAGTGGGTCCTTTGGTAACATCTATGGAAATCTTAAAGCACATTCCTTCCAAGGAATTCC CTGTAACTGAAACACCATTGGTAACTGCAAGAATGATCCTGGAATCCAAAACTGAAAAGAAAATGGTAAG CACTGTTTCTGAATTGGTAACCACAGGTCACTATGGATTCACCTTGGGAGAAGAGGATGATGAAGACAGA ACACTTACAGTTGGATCTGATGAGAGCACCTTGATCTTTGACCAAATTCCTGAAGTCATTACGGTGTCAA AGACTTCAGAAGACACCATCCACACTCATTTAGAAGACTTGGAGTCAGTCTCAGCATCCACAACTGTTTC CCCTTTAATTATGCCTGATAATAATGGATCATCCATGGATGACTGGGAAGAGAGACAAACTAGTGGTAGG ATAACGGAAGAGTTTCTTGGCAAATATCTGTCTACTACACCTTTTCCATCACAGCATCGTACAGAAATAG AATTGTTTCCTTATTCTGGTGATAAAATATTAGTAGAGGGAATTTCCACAGTTATTTATCCTTCTCTACA AACAGAAATGACACATAGAAGAGAAAGAACAGAAACACTAATACCAGAGATGAGAACAGATACTTATACA GATGAAATACAAGAAGAGATCACTAAAAGTCCATTTATGGGAAAAACAGAAGAAGAAGTCTTCTCTGGGA TGAAACTCTCTACATCTCTCTCAGAGCCAATTCATGTTACAGAGTCTTCTGTGGAAATGACCAAGTCTTT TGATTTCCCAACATTGATAACAAAGTTAAGTGCAGAGCCAACAGAAGTAAGAGATATGGAGGAAGACTTT ACAGCAACTCCAGGTACTACAAAATATGATGAAAATATTACAACAGTGCTTTTGGCCCATGGTACTTTAA GTGTTGAAGCAGCCACTGTATCAAAATGGTCATGGGATGAAGATAATACAACATCCAAGCCTTTAGAGTC TACAGAACCTTCAGCCTCTTCAAAATTGCCCCCTGCCTTACTCACAACTGTGGGGATGAATGGAAAGGAT AAAGACATCCCAAGTTTCACTGAAGATGGAGCAGATGAATTTACTCTTATTCCAGATAGTACTCAAAAGC AGTTAGAGGAGGTTACTGATGAAGACATAGCAGCCCATGGAAAATTCACAATTAGATTTCAGCCAACTAC ATCAACTGGTATTGCAGAAAAGTCAACTTTGAGAGATTCTACAACTGAAGAAAAAGTTCCACCTATCACA AGCACTGAAGGCCAAGTTTATGCAACCATGGAAGGAAGTGCTTTGGGTGAAGTAGAAGATGTGGACCTCT CTAAGCCAGTATCTACTGTTCCCCAATTTGCACACACTTCAGAGGTGGAAGGATTAGCATTTGTTAGTTA TAGTAGCACCCAAGAGCCTACTACTTATGTAGACTCTTCCCATACCATTCCTCTTTCTGTAATTCCCAAG ACAGACTGGGGAGTGTTAGTACCTTCTGTTCCATCAGAAGATGAAGTTCTAGGTGAACCCTCTCAAGACA TACTTGTCATTGATCAGACTCGCCTTGAAGCGACTATTTCTCCAGAAACTATGAGAACAACAAAAATCAC AGAGGGAACAACTCAGGAAGAATTCCCTTGGAAAGAACAGACTGCAGAGAAACCAGTTCCTGCTCTCAGT TCTACAGCTTGGACTCCCAAGGAGGCAGTAACACCACTGGATGAACAAGAGGGCGATGGATCAGCATATA CAGTCTCTGAAGATGAATTGTTGACAGGTTCTGAGAGGGTCCCAGTTTTAGAAACAACTCCAGTTGGAAA AATTGATCACAGTGTGTCTTATCCACCAGGTGCTGTAACTGAGCACAAAGTGAAAACAGATGAAGTGGTA ACACTAACACCACGCATTGGGCCAAAAGTATCTTTAAGTCCAGGGCCTGAACAAAAATATGAAACAGAAG GTAGTAGTACAACAGGATTTACATCATCTTTGAGTCCTTTTAGTACCCACATTACCCAGCTTATGGAAGA AACCACTACTGAGAAAACATCCCTAGAGGATATTGATTTAGGCTCAGGATTATTTGAAAAGCCCAAAGCC ACAGAACTCATAGAATTTTCAACAATCAAAGTCACAGTTCCAAGTGATATTACCACTGCCTTCAGTTCAG TAGACAGACTTCACACAACTTCAGCATTCAAGCCATCTTCCGCGATCACTAAGAAACCACCTCTCATCGA CAGGGAACCTGGTGAAGAAACAACCAGTGACATGGTAATCATTGGAGAATCAACATCTCATGTTCCTCCC ACTACCCTTGAAGATATTGTAGCCAAGGAAACAGAAACCGATATTGATAGAGAGTATTTCACGACTTCAA GTCCTCCTGCTACACAGCCAACAAGACCACCCACTGTGGAAGACAAAGAGGCCTTTGGACCTCAGGCGCT TTCTACGCCACAGCCCCCAGCAAGCACAAAATTTCACCCTGACATTAATGTTTATATTATTGAGGTCAGA GAAAATAAGACAGGACCTGATCGCTGCAAAATGAACCCGTGCCTTAACGGAGGCACCTGTTATCCTACTG AAACTTCCTACGTATGCACCTGTGTGCCAGGATACAGCGGAGACCAGTGTGAACTTGATTTTGATGAATG TCACTCTAATCCCTGTCGTAATGGAGCCACTTGTGTTGATGGTTTTAACACATTCAGGTGCCTCTGCCTT CCAAGTTATGTTGGTGCACTTTGTGAGCAAGATACCGAGACATGTGACTATGGCTGGCACAAATTCCAAG GGCAGTGCTACAAATACTTTGCCCATCGACGCACATGGGATGCAGCTGAACGGGAATGCCGTCTGCAGGG TGCCCATCTCACAAGCATCCTGTCTCACGAAGAACAAATGTTTGTTAATCGTGTGGGCCATGATTATCAG TGGATAGGCCTCAATGACAAGATGTTTGAGCATGACTTCCGTTGGACTGATGGCAGCACACTGCAATACG AGAATTGGAGACCCAACCAGCCAGACAGCTTCTTTTCTGCTGGAGAAGACTGTGTTGTAATCATTTGGCA TGAGAATGGCCAGTGGAATGATGTTCCCTGCAATTACCATCTCACCTATACGTGCAAGAAAGGAACAGTC GCTTGCGGCCAGCCCCCTGTTGTAGAAAATGCCAAGACCTTTGGAAAGATGAAACCTCGTTATGAAATCA ACTCCCTGATTAGATACCACTGCAAAGATGGTTTCATTCAACGTCACCTTCCAACTATCCGGTGCTTAGG AAATGGAAGATGGGCTATACCTAAAATTACCTGCATGAACCCATCTGCATACCAAAGGACTTATTCTATG AAATACTTTAAAAATTCCTCATCAGCAAAGGACAATTCAATAAATACATCCAAACATGATCATCGTTGGA GCCGGAGGTGGCAGGAGTCGAGGCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228691 representing NM_001164098
Red=Cloning site Green=Tags(s) MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIE VDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQ DTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPI RAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATV GELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKPKE ATTIDLSILAETASPSLSKEPQMVSDRTTPIIPLVDELPVIPTEFPPVGNIVSFEQKATVQPQAITDSLA TKLPTPTGSTKKPWDMDDYSPSASGPLGKLDISEIKEEVLQSTTGVSHYATDSWDGVVEDKQTQESVTQI EQIEVGPLVTSMEILKHIPSKEFPVTETPLVTARMILESKTEKKMVSTVSELVTTGHYGFTLGEEDDEDR TLTVGSDESTLIFDQIPEVITVSKTSEDTIHTHLEDLESVSASTTVSPLIMPDNNGSSMDDWEERQTSGR ITEEFLGKYLSTTPFPSQHRTEIELFPYSGDKILVEGISTVIYPSLQTEMTHRRERTETLIPEMRTDTYT DEIQEEITKSPFMGKTEEEVFSGMKLSTSLSEPIHVTESSVEMTKSFDFPTLITKLSAEPTEVRDMEEDF TATPGTTKYDENITTVLLAHGTLSVEAATVSKWSWDEDNTTSKPLESTEPSASSKLPPALLTTVGMNGKD KDIPSFTEDGADEFTLIPDSTQKQLEEVTDEDIAAHGKFTIRFQPTTSTGIAEKSTLRDSTTEEKVPPIT STEGQVYATMEGSALGEVEDVDLSKPVSTVPQFAHTSEVEGLAFVSYSSTQEPTTYVDSSHTIPLSVIPK TDWGVLVPSVPSEDEVLGEPSQDILVIDQTRLEATISPETMRTTKITEGTTQEEFPWKEQTAEKPVPALS STAWTPKEAVTPLDEQEGDGSAYTVSEDELLTGSERVPVLETTPVGKIDHSVSYPPGAVTEHKVKTDEVV TLTPRIGPKVSLSPGPEQKYETEGSSTTGFTSSLSPFSTHITQLMEETTTEKTSLEDIDLGSGLFEKPKA TELIEFSTIKVTVPSDITTAFSSVDRLHTTSAFKPSSAITKKPPLIDREPGEETTSDMVIIGESTSHVPP TTLEDIVAKETETDIDREYFTTSSPPATQPTRPPTVEDKEAFGPQALSTPQPPASTKFHPDINVYIIEVR ENKTGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCL PSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQ WIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTV ACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSM KYFKNSSSAKDNSINTSKHDHRWSRRWQESRR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164098 |
| ORF Size | 4926 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164098.1, NP_001157570.1 |
| RefSeq Size | 7154 bp |
| RefSeq ORF | 4929 bp |
| Locus ID | 1462 |
| UniProt ID | P13611 |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| Gene Summary | This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228691 | VCAN (Myc-DDK-tagged)-Human versican (VCAN), transcript variant 4 |
CNY 16,340.00 |
|
| RC228691L3 | Lenti-ORF clone of VCAN (Myc-DDK-tagged)-Human versican (VCAN), transcript variant 4 |
CNY 18,240.00 |
|
| RC228691L4 | Lenti-ORF clone of VCAN (mGFP-tagged)-Human versican (VCAN), transcript variant 4 |
CNY 18,240.00 |
|
| SC327326 | VCAN (untagged)-Human versican (VCAN) transcript variant 4 |
CNY 31,730.00 |
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