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KCNQ5 (NM_001160132) Human Tagged ORF Clone
CAT＃: RG228621
- TrueORF®
KCNQ5 (tGFP-tagged) - Human potassium voltage-gated channel, KQT-like subfamily, member 5 (KCNQ5), transcript variant 3
ORF Plasmid: DDK
"NM_001160132" in other vectors (4)
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CNY 10,740.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Kv7.5; MRD46 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG228621 representing NM_001160132
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCCACCACGCGGGAGGAGAGGAGGGCGGCGCCGCCGGGCTCTGGGTGAAGAGCGGCGCAGCGG CGGCGGCGGCGGGCGGGGGGCGCTTGGGCAGCGGCATGAAGGATGTGGAGTCCGGCCGGGGCAGGGTGCT GCTGAACTCGGCAGCCGCCAGGGGCGACGGCCTGCTACTGCTGGGCACCCGCGCGGCCACGCTCGGTGGC GGCGGCGGTGGCCTGAGGGAGAGCCGCCGGGGCAAGCAGGGGGCCCGGATGAGCCTGCTGGGGAAGCCGC TCTCTTACACGAGTAGCCAGAGCTGCCGGCGCAACGTCAAGTACCGGCGGGTGCAGAACTACCTGTACAA CGTGCTGGAGAGACCCCGCGGCTGGGCGTTCATCTACCACGCTTTCGTTTTTCTCCTTGTCTTTGGTTGC TTGATTTTGTCAGTGTTTTCTACCATCCCTGAGCACACAAAATTGGCCTCAAGTTGCCTCTTGATCCTGG AGTTCGTGATGATTGTCGTCTTTGGTTTGGAGTTCATCATTCGAATCTGGTCTGCGGGTTGCTGTTGTCG ATATAGAGGATGGCAAGGAAGACTGAGGTTTGCTCGAAAGCCCTTCTGTGTTATAGATACCATTGTTCTT ATCGCTTCAATAGCAGTTGTTTCTGCAAAAACTCAGGGTAATATTTTTGCCACGTCTGCACTCAGAAGTC TCCGTTTCCTACAGATCCTCCGCATGGTGCGCATGGACCGAAGGGGAGGCACTTGGAAATTACTGGGTTC AGTGGTTTATGCTCACAGCAAGGAATTAATCACAGCTTGGTACATAGGATTTTTGGTTCTTATTTTTTCG TCTTTCCTTGTCTATCTGGTGGAAAAGGATGCCAATAAAGAGTTTTCTACATATGCAGATGCTCTCTGGT GGGGCACAATTACATTGACAACTATTGGCTATGGAGACAAAACTCCCCTAACTTGGCTGGGAAGATTGCT TTCTGCAGGCTTTGCACTCCTTGGCATTTCTTTCTTTGCACTTCCTGCCGGCATTCTTGGCTCAGGTTTT GCATTAAAAGTACAAGAACAACACCGCCAGAAACACTTTGAGAAAAGAAGGAACCCAGCTGCCAACCTCA TTCAGTGTGTTTGGCGTAGTTACGCAGCTGATGAGAAATCTGTTTCCATTGCAACCTGGAAGCCACACTT GAAGGCCTTGCACACCTGCAGCCCTACCAATAAGTTCTGTAGTAATAAGCAGAAGCTCTTCAGAATGTAC ACCTCACGGAAGCAGAGTCAGAAGCTAAGTTTTAAGGAGCGAGTGCGCATGGCTAGCCCCAGGGGCCAGA GTATTAAGAGCCGACAAGCCTCAGTAGGTGACAGGAGGTCCCCAAGCACCGACATCACAGCCGAGGGCAG TCCCACCAAAGTGCAGAAGAGCTGGAGCTTCAACGACCGAACCCGCTTCCGGCCCTCGCTGCGCCTCAAA AGTTCTCAGCCAAAACCAGTGATAGATGCTGACACAGCCCTTGGCACTGATGATGTATATGATGAAAAAG GATGCCAGTGTGATGTATCAGTGGAAGACCTCACCCCACCACTTAAAACTGTCATTCGAGCTATCAGAAT TATGAAATTTCATGTTGCAAAACGGAAGTTTAAGGAAACATTACGTCCATATGATGTAAAAGATGTCATT GAACAATATTCTGCTGGTCATCTGGACATGTTGTGTAGAATTAAAAGCCTTCAAACACGTGTTGATCAAA TTCTTGGAAAAGGGCAAATCACATCAGATAAGAAGAGCCGAGAGAAAATAACAGCAGAACATGAGACCAC AGACGATCTCAGTATGCTCGGTCGGGTGGTCAAGGTTGAAAAACAGGTACAGTCCATAGAATCCAAGCTG GACTGCCTACTAGACATCTATCAACAGGTCCTTCGGAAAGGCTCTGCCTCAGCCCTCGCTTTGGCTTCAT TCCAGATCCCACCTTTTGAATGTGAACAGACATCTGACTATCAAAGCCCTGTGGATAGCAAAGATCTTTC GGGTTCCGCACAAAACAGTGGCTGCTTATCCAGATCAACTAGTGCCAACATCTCGAGAGGCCTGCAGTTC ATTCTGACGCCAAATGAGTTCAGTGCCCAGACTTTCTACGCGCTTAGCCCTACTATGCACAGTCAAGCAA CACAGGTGCCAATTAGTCAAAGCGATGGCTCAGCAGTGGCAGCCACCAACACCATTGCAAACCAAATAAA TACGGCACCCAAGCCAGCAGCCCCAACAACTTTACAGATCCCACCTCCTCTCCCAGCCATCAAGCATCTG CCCAGGCCAGAAACTCTGCACCCTAACCCTGCAGGCTTACAGGAAAGCATTTCTGACGTCACCACCTGCC TTGTTGCCTCCAAGGAAAATGTTCAGGTTGCACAGTCAAATCTCACCAAGGACCGTTCTATGAGGAAAAG CTTTGACATGGGAGGAGAAACTCTGTTGTCTGTCTGTCCCATGGTGCCGAAGGACTTGGGCAAATCTTTG TCTGTGCAAAACCTGATCAGGTCGACCGAGGAACTGAATATACAACTTTCAGGGAGTGAGTCAAGTGGCT CCAGAGGCAGCCAAGATTTTTACCCCAAATGGAGGGAATCCAAATTGTTTATAACTGATGAAGAGGTGGG TCCCGAAGAGACAGAGACAGACACTTTTGATGCCGCACCGCAGCCTGCCAGGGAAGCTGCCTTTGCATCA GACTCTCTAAGGACTGGAAGGTCACGATCATCTCAGAGCATTTGTAAGGCAGGAGAAAGTACAGATGCCC TCAGCTTGCCTCATGTCAAACTGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228621 representing NM_001160132
Red=Cloning site Green=Tags(s) MPRHHAGGEEGGAAGLWVKSGAAAAAAGGGRLGSGMKDVESGRGRVLLNSAAARGDGLLLLGTRAATLGG GGGGLRESRRGKQGARMSLLGKPLSYTSSQSCRRNVKYRRVQNYLYNVLERPRGWAFIYHAFVFLLVFGC LILSVFSTIPEHTKLASSCLLILEFVMIVVFGLEFIIRIWSAGCCCRYRGWQGRLRFARKPFCVIDTIVL IASIAVVSAKTQGNIFATSALRSLRFLQILRMVRMDRRGGTWKLLGSVVYAHSKELITAWYIGFLVLIFS SFLVYLVEKDANKEFSTYADALWWGTITLTTIGYGDKTPLTWLGRLLSAGFALLGISFFALPAGILGSGF ALKVQEQHRQKHFEKRRNPAANLIQCVWRSYAADEKSVSIATWKPHLKALHTCSPTNKFCSNKQKLFRMY TSRKQSQKLSFKERVRMASPRGQSIKSRQASVGDRRSPSTDITAEGSPTKVQKSWSFNDRTRFRPSLRLK SSQPKPVIDADTALGTDDVYDEKGCQCDVSVEDLTPPLKTVIRAIRIMKFHVAKRKFKETLRPYDVKDVI EQYSAGHLDMLCRIKSLQTRVDQILGKGQITSDKKSREKITAEHETTDDLSMLGRVVKVEKQVQSIESKL DCLLDIYQQVLRKGSASALALASFQIPPFECEQTSDYQSPVDSKDLSGSAQNSGCLSRSTSANISRGLQF ILTPNEFSAQTFYALSPTMHSQATQVPISQSDGSAVAATNTIANQINTAPKPAAPTTLQIPPPLPAIKHL PRPETLHPNPAGLQESISDVTTCLVASKENVQVAQSNLTKDRSMRKSFDMGGETLLSVCPMVPKDLGKSL SVQNLIRSTEELNIQLSGSESSGSRGSQDFYPKWRESKLFITDEEVGPEETETDTFDAAPQPAREAAFAS DSLRTGRSRSSQSICKAGESTDALSLPHVKLK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001160132 |
| ORF Size | 2826 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001160132.2 |
| RefSeq Size | 6625 bp |
| RefSeq ORF | 2829 bp |
| Locus ID | 56479 |
| UniProt ID | Q9NR82 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| Gene Summary | This gene is a member of the KCNQ potassium channel gene family that is differentially expressed in subregions of the brain and in skeletal muscle. The protein encoded by this gene yields currents that activate slowly with depolarization and can form heteromeric channels with the protein encoded by the KCNQ3 gene. Currents expressed from this protein have voltage dependences and inhibitor sensitivities in common with M-currents. They are also inhibited by M1 muscarinic receptor activation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228621 | KCNQ5 (Myc-DDK-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 5 (KCNQ5), transcript variant 3 |
CNY 9,790.00 |
|
| RC228621L3 | Lenti-ORF clone of KCNQ5 (Myc-DDK-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 5 (KCNQ5), transcript variant 3 |
CNY 11,690.00 |
|
| RC228621L4 | Lenti-ORF clone of KCNQ5 (mGFP-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 5 (KCNQ5), transcript variant 3 |
CNY 11,690.00 |
|
| SC327256 | KCNQ5 (untagged)-Human potassium voltage-gated channel KQT-like subfamily member 5 (KCNQ5) transcript variant 3 |
CNY 18,240.00 |
