United States
Germany
Japan
United Kingdom
China
PIWIL2 (NM_001135721) Human Tagged ORF Clone
CAT＃: RG227303
- TrueORF®
PIWIL2 (tGFP-tagged) - Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 1
ORF Plasmid: DDK
"NM_001135721" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CT80; HILI; mili; PIWIL1L |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227303 representing NM_001135721
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCTTTCCGACCATCGTTCAGGGGCCAGTCTCCTATCCACCCATCCCAGTGCCAGGCTGTACGGA TGCCAGGCTGTTGGCCACAAGCTTCTAAACCTTTGGACCCAGCTCTGGGCAGGGGAGCACCTGCAGGCAG AGGCCATGTATTTGGAAAGCCAGAGGAACCAAGCACACAGAGGGGGCCAGCACAAAGGGAGTCTGTGGGT TTGGTCTCCATGTTCCGAGGCCTGGGCATTGAAACAGTTTCTAAGACCCCTCTGAAACGGGAAATGCTTC CATCAGGTAGAGGCATTTTAGGTCGAGGCTTGTCTGCTAATCTGGTACGCAAGGACAGGGAGGAACTCTC TCCCACTTTTTGGGATCCAAAAGTGTTGGCGGCTGGGGACAGCAAGATGGCAGAGACCTCCGTTGGTTGG AGTAGGACGCTTGGAAGAGGGAGTTCAGATGCGTCTTTATTACCACTGGGAAGAGCAGCAGGTGGTATCA GCAGAGAAGTGGACAAGCCTCCCTGTACCTTCAGCACACCGTCCCGGGGTCCCCCGCAGCTGTCATCACC ACCAGCTCTGCCCCAGTCTCCCCTGCACTCTCCAGATCGCCCTCTGGTCCTGACTGTGGAACACAAGGAA AAAGAGCTTATTGTGAAGCAAGGATCAAAAGGAACACCTCAGTCTTTGGGACTGAACCTCGTCAAAATAC AGTGTCATAATGAAGCAGTTTATCAATATCATGTGACTTTCAGCCCCAATGTGGAGTGCAAAAGCATGAG GTTCGGCATGTTGAAGGACCATCAAGCTGTCACCGGCAACGTCACTGCGTTTGATGGATCTATTCTCTAT CTGCCTGTTAAGCTTCAACAAGTTCTTGAGTTAAAAAGTCAAAGGAAAACAGACAGTGCTGAAATCAGCA TTAAGATTCAGATGACAAAGATCCTGGAGCCCTGCTCTGACCTGTGCATTCCCTTCTACAATGTTGTTTT CCGTCGGGTAATGAAACTTTTAGATATGAAGCTTGTGGGGAGAAACTTTTATGACCCTACAAGTGCTATG GTACTACAGCAACACAGATTGCAGATCTGGCCAGGCTATGCAGCTAGCATCCGAAGGACAGATGGAGGGC TCTTCCTGCTAGCTGATGTCTCCCATAAGGTCATTCGGAATGACTGTGTGCTGGATGTCATGCATGCCAT TTATCAGCAGAATAAAGAACACTTCCAGGATGAGTGTACTAAGCTTCTGGTTGGCAATATTGTTATCACC CGATATAACAATCGTACCTATCGTATTGATGATGTGGATTGGAATAAGACTCCAAAGGATAGCTTCACGA TGTCTGATGGGAAAGAGATCACATTCTTGGAATACTACAGCAAAAATTATGGGATCACAGTTAAGGAAGA GGACCAGCCATTGCTGATTCACAGGCCCAGTGAGAGACAGGATAATCATGGGATGCTGCTAAAAGGGGAA ATCCTGCTGCTGCCTGAGCTTTCTTTTATGACCGGAATCCCAGAGAAGATGAAGAAGGACTTCAGAGCCA TGAAGGATTTGGCTCAGCAAATCAATCTGAGCCCCAAGCAACACCATAGTGCTTTGGAATGCTTGCTGCA AAGAATTGCAAAGAACGAGGCAGCCACCAATGAACTGATGCGTTGGGGGCTCCGTCTGCAAAAGGATGTA CATAAGATTGAAGGACGTGTTCTGCCAATGGAAAGAATTAACTTAAAAAATACTTCGTTTATCACATCTC AGGAACTAAACTGGGTTAAGGAAGTAACCAGAGACCCTTCCATCTTGACTATCCCCATGCATTTCTGGGC ACTTTTTTACCCAAAGAGAGCAATGGACCAGGCTCGAGAACTGGTCAACATGTTGGAGAAGATAGCCGGC CCCATTGGCATGCGTATGAGCCCACCGGCCTGGGTTGAACTAAAGGATGACCGAATAGAGACTTATGTCA GAACCATTCAATCCACGTTAGGAGCTGAGGGGAAGATACAGATGGTTGTTTGCATCATCATGGGCCCACG TGATGATCTCTATGGGGCCATCAAGAAGCTGTGCTGTGTGCAGTCCCCAGTGCCCTCCCAGGTTGTCAAT GTTCGAACCATTGGTCAGCCCACCAGGCTTCGGAGTGTGGCCCAGAAGATTTTACTTCAGATTAACTGTA AATTGGGTGGTGAGCTCTGGGGAGTGGATATTCCTCTGAAACAGTTAATGGTGATCGGGATGGATGTTTA CCATGACCCCAGTAGAGGCATGCGCTCCGTGGTTGGCTTCGTGGCAAGCATCAATCTCACCCTCACAAAA TGGTATTCCCGGGTGGTGTTCCAGATGCCGCATCAGGAGATTGTGGACAGCCTGAAGCTATGCCTCGTGG GCTCCTTAAAAAAGTTTTATGAGGTGAACCACTGTCTACCAGAGAAGATTGTGGTGTACCGTGATGGAGT GTCTGATGGCCAACTGAAGACAGTTGCCAACTATGAGATTCCTCAACTACAGAAGTGTTTTGAAGCTTTT GAGAATTATCAGCCCAAGATGGTGGTGTTTGTAGTTCAGAAGAAAATCAGTACTAATCTATATCTGGCTG CTCCTCAGAACTTTGTAACTCCCACTCCTGGAACTGTGGTAGATCATACAATAACAAGCTGTGAGTGGGT GGATTTCTATCTTCTTGCCCATCATGTACGGCAGGGCTGTGGCATTCCTACGCATTATGTCTGTGTTCTC AACACCGCAAACCTGAGCCCTGATCATATGCAGAGGCTGACTTTCAAACTGTGCCACATGTACTGGAATT GGCCTGGCACCATCAGAGTTCCAGCTCCTTGCAAGTATGCCCACAAGCTAGCTTTCCTGTCAGGACACAT CTTGCATCATGAACCAGCCATCCAGCTGTGCGAGAACCTGTTCTTCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227303 representing NM_001135721
Red=Cloning site Green=Tags(s) MDPFRPSFRGQSPIHPSQCQAVRMPGCWPQASKPLDPALGRGAPAGRGHVFGKPEEPSTQRGPAQRESVG LVSMFRGLGIETVSKTPLKREMLPSGRGILGRGLSANLVRKDREELSPTFWDPKVLAAGDSKMAETSVGW SRTLGRGSSDASLLPLGRAAGGISREVDKPPCTFSTPSRGPPQLSSPPALPQSPLHSPDRPLVLTVEHKE KELIVKQGSKGTPQSLGLNLVKIQCHNEAVYQYHVTFSPNVECKSMRFGMLKDHQAVTGNVTAFDGSILY LPVKLQQVLELKSQRKTDSAEISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAM VLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDCVLDVMHAIYQQNKEHFQDECTKLLVGNIVIT RYNNRTYRIDDVDWNKTPKDSFTMSDGKEITFLEYYSKNYGITVKEEDQPLLIHRPSERQDNHGMLLKGE ILLLPELSFMTGIPEKMKKDFRAMKDLAQQINLSPKQHHSALECLLQRIAKNEAATNELMRWGLRLQKDV HKIEGRVLPMERINLKNTSFITSQELNWVKEVTRDPSILTIPMHFWALFYPKRAMDQARELVNMLEKIAG PIGMRMSPPAWVELKDDRIETYVRTIQSTLGAEGKIQMVVCIIMGPRDDLYGAIKKLCCVQSPVPSQVVN VRTIGQPTRLRSVAQKILLQINCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTK WYSRVVFQMPHQEIVDSLKLCLVGSLKKFYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAF ENYQPKMVVFVVQKKISTNLYLAAPQNFVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYVCVL NTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHHEPAIQLCENLFFL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135721 |
| ORF Size | 2919 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001135721.2 |
| RefSeq Size | 3997 bp |
| RefSeq ORF | 2922 bp |
| Locus ID | 55124 |
| UniProt ID | Q8TC59 |
| Protein Pathways | Dorso-ventral axis formation |
| Gene Summary | PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227303 | PIWIL2 (Myc-DDK-tagged)-Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 1 |
CNY 7,128.00 |
|
| RC227303L1 | Lenti ORF clone of Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC227303L2 | Lenti ORF clone of Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| RC227303L3 | Lenti ORF clone of Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC227303L4 | Lenti ORF clone of Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| SC325382 | PIWIL2 (untagged)-Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 1 |
CNY 7,220.00 |
