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KCTD1 (NM_001142730) Human Tagged ORF Clone
CAT＃: RG227186
- TrueORF®
KCTD1 (tGFP-tagged) - Human potassium channel tetramerisation domain containing 1 (KCTD1), transcript variant 3
ORF Plasmid: DDK
"NM_001142730" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C18orf5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227186 representing NM_001142730
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGAATGCCTGGCAGCGGGGACTGTAACACCAGCGCGGGCGGCAGCGCCAGCGCTGCCGCCGCCG CCGCCGAGAACAATGGGGAGCGGGGCGAGGGCGAGCGCGGCGCGGGGGGCCGCGGCCGCCGCCACAGCCG TCCGCACTACTGCAGCGCGGGCGAGGAGGAGGAGGAAGAGGAGGAGGAGGACGAGATCCAGGAGGTGCAG ATAACGGGGGACGAGGAGGAGGAGGAGGACGGAGGTGGGGGGCTGGAGGAGGACGAGGAGGAGGAGGAAG AGGAGGAGATGGGGCTGGACTGGGACGAGCCCCTGGAGCCCGAGGACTCGGCCGGGGAGGAGCTGGAGCC CGAGCCGGTCCATATGATCAATATGGACCAGAGCGCCGCGCTGGAGCCCGAGGCGCCGCCGCGACTGCTG GCGCCCCGGGCCCGCGGTGGGCCGCCCGGGGACGGCTCCGAGCTGGACCCCGACGTGCTGCAGCGCCCCG AGCGGGCCCGGCTGAGCGAGAACACCCGGCTGGCCACCCGCTACGCCGTGCGCATCTTCCGGGAGTACCT GAGCGAGAAGGCGCAGAGCCCGGACTTCGAGACCATGGACAAGGGGGCGCTGTGCCGCGTGCTGCGCTCC TTCTATGCCGAGGCCCGCTCCAAAAGCGGCCAGCTCTACAGCAAGTCGTCGCTCATCAGCATCCGCAGCT CCCTCAACCGCTACCTCAATGAGCCCCCGTACTGCCGCACGCTCGACCTCACCAAGGACCCCGAGCTGCG CAGCGCCAACCTGACGCTGGCCGCGGTCATCCGCAAGCTCGAGGAGCAGGGCGCCGGGCCGGTGGTGCAG AAGCAAGCCATCACGCGCGCCGACCTGCGCAAGCTGTACACCTCCAGCGTCTTCAGCACCAACACGCCCT TCGGGCTGCTCAACAAGGTCTGGTTCGAGACGTGCATGTACTTCTGTACCCGCGGCCGCGAGAACCAGCG TGAGTTGGAGGAGGACTCTTTTGGGCTGGCCATGGACGAGGACGGTCGCAAGTTCGTCTACTTCAAGTCC CTCGGGCCCTACCACAAGTCGCGCTCGTCGTCGTGGAGCAAGAAGCGCGCCGAGAGCAGCGACGAGGAGA ACTTGCCCCGCATGTATGAGACGGGCACCGAGTTCTGCCCCTACGCCAGCTTCGTCAAGTACCTGTCGAA ACGCAACCCTCTCTGCAAGGCGTTCTTCCAGCGGCCCCGGGACCACTGCAGCGAGGGCGATGTGACCTGG TACGAGAACAAAGCCATCGGCAAGAACTTGCTAGGCACTCGGATGCAGATGCTCTCCAAGGCGGCCAAGC TCTCCAAGACCTACACCAACCACTGCATCGGCGCCGTCTCCATCGCCACGCTCAACAGCATCGCGGGCAT TGGCACCAAGCTGGGCTCGCCCGCCCCGCAGGGCTGCTACGCCGAGGCTCTGAACGGGGCGGCACGGCAC CACTCCCACCACCCCCCCACCCATCCCTCCCACCACCACCGCCCCCAGCCGCCCTCGCTGGGGAACACTT ACATCCTCCCCAAAGACAGCCAGGTCGGGCCCGACGTGAAATCCGAGGCTGCGCCCAAGCGCGCCCTGTA CGAGTCTGTGTTCGGGTCGGGGGAAATCTGCGGCCCCACTTCCCCCAAAAGACTTTGTATCCGCCCCTCG GAGCCTGTGGATGCGGTGGTGGTGGTTTCCGTGAAACACGACCCCCTGCCTCTTCTTCCAGAAGCCAATG GGCACAGAAGCACCAATTCTCCCACAATAGTTTCACCTGCTATTGTTTCCCCCACCCAGGACAGTCGGCC CAATATGTCAAGACCTCTGATCACTAGATCCCCTGCATCTCCACTGAACAACCAAGGCATCCCTACTCCA GCACAACTCACAAAATCCAATGCGCCTGTCCACATTGATGTGGGCGGCCACATGTACACCAGCAGCCTGG CCACCCTCACCAAATACCCTGAATCCAGAATCGGAAGACTTTTTGATGGTACAGAGCCCATTGTTTTGGA CAGTCTCAAACAGCACTATTTCATTGACAGAGATGGACAGATGTTCAGATATATCTTGAATTTTCTACGA ACATCCAAACTCCTCATTCCTGATGATTTCAAGGACTACACTTTGTTATATGAAGAGGCAAAATATTTTC AGCTTCAGCCCATGTTGTTGGAGATGGAAAGATGGAAGCAGGACAGAGAAACTGGTCGATTTTCAAGGCC CTGTGAGTGCCTCGTCGTGCGTGTGGCCCCAGACCTCGGAGAAAGGATCACGCTAAGCGGTGACAAATCC TTGATAGAAGAAGTATTTCCAGAGATCGGCGACGTGATGTGTAACTCTGTCAATGCAGGCTGGAATCACG ACTCGACGCACGTCATCAGGTTTCCACTAAATGGCTACTGTCACCTCAACTCAGTCCAGGTCCTCGAGAG GTTGCAGCAAAGAGGATTTGAAATCGTGGGCTCCTGTGGGGGAGGAGTAGACTCGTCCCAGTTCAGCGAA TACGTCCTTCGGCGGGAACTGAGGCGGACGCCCCGTGTACCCTCCGTCATCCGGATAAAGCAAGAGCCTC TGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227186 representing NM_001142730
Red=Cloning site Green=Tags(s) MARMPGSGDCNTSAGGSASAAAAAAENNGERGEGERGAGGRGRRHSRPHYCSAGEEEEEEEEEDEIQEVQ ITGDEEEEEDGGGGLEEDEEEEEEEEMGLDWDEPLEPEDSAGEELEPEPVHMINMDQSAALEPEAPPRLL APRARGGPPGDGSELDPDVLQRPERARLSENTRLATRYAVRIFREYLSEKAQSPDFETMDKGALCRVLRS FYAEARSKSGQLYSKSSLISIRSSLNRYLNEPPYCRTLDLTKDPELRSANLTLAAVIRKLEEQGAGPVVQ KQAITRADLRKLYTSSVFSTNTPFGLLNKVWFETCMYFCTRGRENQRELEEDSFGLAMDEDGRKFVYFKS LGPYHKSRSSSWSKKRAESSDEENLPRMYETGTEFCPYASFVKYLSKRNPLCKAFFQRPRDHCSEGDVTW YENKAIGKNLLGTRMQMLSKAAKLSKTYTNHCIGAVSIATLNSIAGIGTKLGSPAPQGCYAEALNGAARH HSHHPPTHPSHHHRPQPPSLGNTYILPKDSQVGPDVKSEAAPKRALYESVFGSGEICGPTSPKRLCIRPS EPVDAVVVVSVKHDPLPLLPEANGHRSTNSPTIVSPAIVSPTQDSRPNMSRPLITRSPASPLNNQGIPTP AQLTKSNAPVHIDVGGHMYTSSLATLTKYPESRIGRLFDGTEPIVLDSLKQHYFIDRDGQMFRYILNFLR TSKLLIPDDFKDYTLLYEEAKYFQLQPMLLEMERWKQDRETGRFSRPCECLVVRVAPDLGERITLSGDKS LIEEVFPEIGDVMCNSVNAGWNHDSTHVIRFPLNGYCHLNSVQVLERLQQRGFEIVGSCGGGVDSSQFSE YVLRRELRRTPRVPSVIRIKQEPLD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001142730 |
| ORF Size | 2595 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001142730.3 |
| RefSeq Size | 3438 bp |
| RefSeq ORF | 2598 bp |
| Locus ID | 284252 |
| UniProt ID | Q719H9 |
| Protein Families | Ion Channels: Other |
| Gene Summary | This gene encodes a protein containing a BTB (Broad-complex, tramtrack and bric a brac), also known as a POZ (POxvirus and zinc finger) protein-protein interaction domain. The encoded protein negatively regulates the AP-2 family of transcription factors and the Wnt signaling pathway. A mechanism for the modulation of Wnt signaling has been proposed in which the encoded protein enhances ubiquitination and degradation of the beta-catenin protein. Mutations in this gene have been identified in Scalp-ear-nipple (SEN) syndrome. [provided by RefSeq, May 2017] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227186 | KCTD1 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 1 (KCTD1), transcript variant 3 |
CNY 7,130.00 |
|
| RC227186L3 | Lenti-ORF clone of KCTD1 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 1 (KCTD1), transcript variant 3 |
CNY 9,030.00 |
|
| RC227186L4 | Lenti-ORF clone of KCTD1 (mGFP-tagged)-Human potassium channel tetramerisation domain containing 1 (KCTD1), transcript variant 3 |
CNY 9,030.00 |
|
| SC326263 | KCTD1 (untagged)-Human potassium channel tetramerisation domain containing 1 (KCTD1), transcript variant 3 |
CNY 16,720.00 |
